Mucus threads from surface goblet cells clear particles from the airways

Abstract Background The mucociliary clearance system driven by beating cilia protects the airways from inhaled microbes and particles. Large particles are cleared by mucus bundles made in submucosal glands by parallel linear polymers of the MUC5B mucins. However, the structural organization and func...

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Autores principales:	Anna Ermund, Lauren N. Meiss, Brendan Dolan, Florian Jaudas, Lars Ewaldsson, Andrea Bähr, Nikolai Klymiuk, Gunnar C. Hansson
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Mucins Respiratory tract Mucus bundle Goblet cells Trachea Bronchi Diseases of the respiratory system RC705-779
Acceso en línea:	https://doaj.org/article/ddf81e3cc48d4be383cadbc953dd2e50
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spelling	oai:doaj.org-article:ddf81e3cc48d4be383cadbc953dd2e502021-11-28T12:29:17ZMucus threads from surface goblet cells clear particles from the airways10.1186/s12931-021-01898-31465-993Xhttps://doaj.org/article/ddf81e3cc48d4be383cadbc953dd2e502021-11-01T00:00:00Zhttps://doi.org/10.1186/s12931-021-01898-3https://doaj.org/toc/1465-993XAbstract Background The mucociliary clearance system driven by beating cilia protects the airways from inhaled microbes and particles. Large particles are cleared by mucus bundles made in submucosal glands by parallel linear polymers of the MUC5B mucins. However, the structural organization and function of the mucus generated in surface goblet cells are poorly understood. Methods The origin and characteristics of different mucus structures were studied on live tissue explants from newborn wild-type (WT), cystic fibrosis transmembrane conductance regulator (CFTR) deficient (CF) piglets and weaned pig airways using video microscopy, Airyscan imaging and electron microscopy. Bronchoscopy was performed in juvenile pigs in vivo. Results We have identified a distinct mucus formation secreted from the surface goblet cells with a diameter less than two micrometer. This type of mucus was named mucus threads. With time mucus threads gathered into larger mucus assemblies, efficiently collecting particles. The previously observed Alcian blue stained mucus bundles were around 10 times thicker than the threads. Together the mucus bundles, mucus assemblies and mucus threads cleared the pig trachea from particles. Conclusions These results demonstrate that normal airway mucus is more complex and has a more variable structural organization and function than was previously understood. These observations emphasize the importance of studying young objects to understand the function of a non-compromised lung.Anna ErmundLauren N. MeissBrendan DolanFlorian JaudasLars EwaldssonAndrea BährNikolai KlymiukGunnar C. HanssonBMCarticleMucinsRespiratory tractMucus bundleGoblet cellsTracheaBronchiDiseases of the respiratory systemRC705-779ENRespiratory Research, Vol 22, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Mucins Respiratory tract Mucus bundle Goblet cells Trachea Bronchi Diseases of the respiratory system RC705-779
spellingShingle	Mucins Respiratory tract Mucus bundle Goblet cells Trachea Bronchi Diseases of the respiratory system RC705-779 Anna Ermund Lauren N. Meiss Brendan Dolan Florian Jaudas Lars Ewaldsson Andrea Bähr Nikolai Klymiuk Gunnar C. Hansson Mucus threads from surface goblet cells clear particles from the airways
description	Abstract Background The mucociliary clearance system driven by beating cilia protects the airways from inhaled microbes and particles. Large particles are cleared by mucus bundles made in submucosal glands by parallel linear polymers of the MUC5B mucins. However, the structural organization and function of the mucus generated in surface goblet cells are poorly understood. Methods The origin and characteristics of different mucus structures were studied on live tissue explants from newborn wild-type (WT), cystic fibrosis transmembrane conductance regulator (CFTR) deficient (CF) piglets and weaned pig airways using video microscopy, Airyscan imaging and electron microscopy. Bronchoscopy was performed in juvenile pigs in vivo. Results We have identified a distinct mucus formation secreted from the surface goblet cells with a diameter less than two micrometer. This type of mucus was named mucus threads. With time mucus threads gathered into larger mucus assemblies, efficiently collecting particles. The previously observed Alcian blue stained mucus bundles were around 10 times thicker than the threads. Together the mucus bundles, mucus assemblies and mucus threads cleared the pig trachea from particles. Conclusions These results demonstrate that normal airway mucus is more complex and has a more variable structural organization and function than was previously understood. These observations emphasize the importance of studying young objects to understand the function of a non-compromised lung.
format	article
author	Anna Ermund Lauren N. Meiss Brendan Dolan Florian Jaudas Lars Ewaldsson Andrea Bähr Nikolai Klymiuk Gunnar C. Hansson
author_facet	Anna Ermund Lauren N. Meiss Brendan Dolan Florian Jaudas Lars Ewaldsson Andrea Bähr Nikolai Klymiuk Gunnar C. Hansson
author_sort	Anna Ermund
title	Mucus threads from surface goblet cells clear particles from the airways
title_short	Mucus threads from surface goblet cells clear particles from the airways
title_full	Mucus threads from surface goblet cells clear particles from the airways
title_fullStr	Mucus threads from surface goblet cells clear particles from the airways
title_full_unstemmed	Mucus threads from surface goblet cells clear particles from the airways
title_sort	mucus threads from surface goblet cells clear particles from the airways
publisher	BMC
publishDate	2021
url	https://doaj.org/article/ddf81e3cc48d4be383cadbc953dd2e50
work_keys_str_mv	AT annaermund mucusthreadsfromsurfacegobletcellsclearparticlesfromtheairways AT laurennmeiss mucusthreadsfromsurfacegobletcellsclearparticlesfromtheairways AT brendandolan mucusthreadsfromsurfacegobletcellsclearparticlesfromtheairways AT florianjaudas mucusthreadsfromsurfacegobletcellsclearparticlesfromtheairways AT larsewaldsson mucusthreadsfromsurfacegobletcellsclearparticlesfromtheairways AT andreabahr mucusthreadsfromsurfacegobletcellsclearparticlesfromtheairways AT nikolaiklymiuk mucusthreadsfromsurfacegobletcellsclearparticlesfromtheairways AT gunnarchansson mucusthreadsfromsurfacegobletcellsclearparticlesfromtheairways
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Mucus threads from surface goblet cells clear particles from the airways

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