Expression of pluripotency master regulators during two key developmental transitions: EGA and early lineage specification in the bovine embryo.

Pluripotency genes are implicated in mouse embryonic genome activation (EGA) and pluripotent lineage specification. Moreover, their expression levels have been correlated with embryonic term development. In bovine, however, little information is available about dynamics of pluripotency genes during...

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Autores principales:	Daulat Raheem Khan, Delphine Dubé, Laurence Gall, Nathalie Peynot, Sylvie Ruffini, Ludivine Laffont, Daniel Le Bourhis, Séverine Degrelle, Alice Jouneau, Véronique Duranthon
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ddfa5d2a76824285a686a0eab6d50d2d
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spelling	oai:doaj.org-article:ddfa5d2a76824285a686a0eab6d50d2d2021-11-18T07:23:51ZExpression of pluripotency master regulators during two key developmental transitions: EGA and early lineage specification in the bovine embryo.1932-620310.1371/journal.pone.0034110https://doaj.org/article/ddfa5d2a76824285a686a0eab6d50d2d2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22479535/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Pluripotency genes are implicated in mouse embryonic genome activation (EGA) and pluripotent lineage specification. Moreover, their expression levels have been correlated with embryonic term development. In bovine, however, little information is available about dynamics of pluripotency genes during these processes. In this study, we charted quantitative and/or qualitative spatio-temporal expression patterns of transcripts and proteins of pluripotency genes (OCT4, SOX2 and NANOG) and mRNA levels of some of their downstream targets in bovine oocytes and early embryos. Furthermore, to correlate expression patterns of these genes with term developmental potential, we used cloned embryos, having similar in vitro but different full term development rates. Our findings affirm: firstly, the core triad of pluripotency genes is probably not implicated in bovine EGA since their proteins were not detected during pre-EGA phase, despite the transcripts for OCT4 and SOX2 were present. Secondly, an earlier ICM specification of transcripts and proteins of SOX2 and NANOG makes them pertinent candidates of bovine pluripotent lineage specification than OCT4. Thirdly, embryos with low term development potential have higher transcription rates; nevertheless, precarious balance between pluripotency genes is maintained. This balance presages normal in vitro development but, probably higher transcription rate disturbs it at later stage that abrogates term development.Daulat Raheem KhanDelphine DubéLaurence GallNathalie PeynotSylvie RuffiniLudivine LaffontDaniel Le BourhisSéverine DegrelleAlice JouneauVéronique DuranthonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 3, p e34110 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daulat Raheem Khan Delphine Dubé Laurence Gall Nathalie Peynot Sylvie Ruffini Ludivine Laffont Daniel Le Bourhis Séverine Degrelle Alice Jouneau Véronique Duranthon Expression of pluripotency master regulators during two key developmental transitions: EGA and early lineage specification in the bovine embryo.
description	Pluripotency genes are implicated in mouse embryonic genome activation (EGA) and pluripotent lineage specification. Moreover, their expression levels have been correlated with embryonic term development. In bovine, however, little information is available about dynamics of pluripotency genes during these processes. In this study, we charted quantitative and/or qualitative spatio-temporal expression patterns of transcripts and proteins of pluripotency genes (OCT4, SOX2 and NANOG) and mRNA levels of some of their downstream targets in bovine oocytes and early embryos. Furthermore, to correlate expression patterns of these genes with term developmental potential, we used cloned embryos, having similar in vitro but different full term development rates. Our findings affirm: firstly, the core triad of pluripotency genes is probably not implicated in bovine EGA since their proteins were not detected during pre-EGA phase, despite the transcripts for OCT4 and SOX2 were present. Secondly, an earlier ICM specification of transcripts and proteins of SOX2 and NANOG makes them pertinent candidates of bovine pluripotent lineage specification than OCT4. Thirdly, embryos with low term development potential have higher transcription rates; nevertheless, precarious balance between pluripotency genes is maintained. This balance presages normal in vitro development but, probably higher transcription rate disturbs it at later stage that abrogates term development.
format	article
author	Daulat Raheem Khan Delphine Dubé Laurence Gall Nathalie Peynot Sylvie Ruffini Ludivine Laffont Daniel Le Bourhis Séverine Degrelle Alice Jouneau Véronique Duranthon
author_facet	Daulat Raheem Khan Delphine Dubé Laurence Gall Nathalie Peynot Sylvie Ruffini Ludivine Laffont Daniel Le Bourhis Séverine Degrelle Alice Jouneau Véronique Duranthon
author_sort	Daulat Raheem Khan
title	Expression of pluripotency master regulators during two key developmental transitions: EGA and early lineage specification in the bovine embryo.
title_short	Expression of pluripotency master regulators during two key developmental transitions: EGA and early lineage specification in the bovine embryo.
title_full	Expression of pluripotency master regulators during two key developmental transitions: EGA and early lineage specification in the bovine embryo.
title_fullStr	Expression of pluripotency master regulators during two key developmental transitions: EGA and early lineage specification in the bovine embryo.
title_full_unstemmed	Expression of pluripotency master regulators during two key developmental transitions: EGA and early lineage specification in the bovine embryo.
title_sort	expression of pluripotency master regulators during two key developmental transitions: ega and early lineage specification in the bovine embryo.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/ddfa5d2a76824285a686a0eab6d50d2d
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Expression of pluripotency master regulators during two key developmental transitions: EGA and early lineage specification in the bovine embryo.

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