Nocardia brasiliensis induces formation of foamy macrophages and dendritic cells in vitro and in vivo.

Foamy cells have been described in various infectious diseases, for example in actinomycetoma induced by Nocardia brasiliensis. These cells are generally considered to be macrophages, although they present dendritic cell (DC)-specific surface markers. In this study, we determined and confirmed the l...

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Autores principales:	Irene Meester, Adrian Geovanni Rosas-Taraco, Mario Cesar Salinas-Carmona
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/de026d494eb14a9bab0265804346ad7b
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spelling	oai:doaj.org-article:de026d494eb14a9bab0265804346ad7b2021-11-18T08:15:24ZNocardia brasiliensis induces formation of foamy macrophages and dendritic cells in vitro and in vivo.1932-620310.1371/journal.pone.0100064https://doaj.org/article/de026d494eb14a9bab0265804346ad7b2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24936860/?tool=EBIhttps://doaj.org/toc/1932-6203Foamy cells have been described in various infectious diseases, for example in actinomycetoma induced by Nocardia brasiliensis. These cells are generally considered to be macrophages, although they present dendritic cell (DC)-specific surface markers. In this study, we determined and confirmed the lineage of possible precursors of foamy cells in vitro and in vivo using an experimental actinomycetoma model in BALB/c mice. Bone marrow-derived macrophages (BMDM) or DC (BMDC) were infected in vitro with N. brasiliensis or labeled with carboxyfluorescein diacetate succinimidyl ester (CFSE). Both, macrophages and DC, differentiated into foamy cells after in vitro infection. CFSE-labeled BMDM or BMDC were tested for phagocytosis and CD11c/CD11b receptors markers expression before being transferred into the actinomycetoma lesion site of infected mice. In vivo studies showed that BMDM and BMDC were traced at the site where foamy cells are present in the experimental actinomycetoma. Interestingly, many of the transferred BMDM and BMDC were stained with the lipid-droplet fluorophore Nile Red. In conclusion, macrophages and DC cells can be differentiated into foamy cells in vitro and in vivo during N. brasiliensis infection.Irene MeesterAdrian Geovanni Rosas-TaracoMario Cesar Salinas-CarmonaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e100064 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Irene Meester Adrian Geovanni Rosas-Taraco Mario Cesar Salinas-Carmona Nocardia brasiliensis induces formation of foamy macrophages and dendritic cells in vitro and in vivo.
description	Foamy cells have been described in various infectious diseases, for example in actinomycetoma induced by Nocardia brasiliensis. These cells are generally considered to be macrophages, although they present dendritic cell (DC)-specific surface markers. In this study, we determined and confirmed the lineage of possible precursors of foamy cells in vitro and in vivo using an experimental actinomycetoma model in BALB/c mice. Bone marrow-derived macrophages (BMDM) or DC (BMDC) were infected in vitro with N. brasiliensis or labeled with carboxyfluorescein diacetate succinimidyl ester (CFSE). Both, macrophages and DC, differentiated into foamy cells after in vitro infection. CFSE-labeled BMDM or BMDC were tested for phagocytosis and CD11c/CD11b receptors markers expression before being transferred into the actinomycetoma lesion site of infected mice. In vivo studies showed that BMDM and BMDC were traced at the site where foamy cells are present in the experimental actinomycetoma. Interestingly, many of the transferred BMDM and BMDC were stained with the lipid-droplet fluorophore Nile Red. In conclusion, macrophages and DC cells can be differentiated into foamy cells in vitro and in vivo during N. brasiliensis infection.
format	article
author	Irene Meester Adrian Geovanni Rosas-Taraco Mario Cesar Salinas-Carmona
author_facet	Irene Meester Adrian Geovanni Rosas-Taraco Mario Cesar Salinas-Carmona
author_sort	Irene Meester
title	Nocardia brasiliensis induces formation of foamy macrophages and dendritic cells in vitro and in vivo.
title_short	Nocardia brasiliensis induces formation of foamy macrophages and dendritic cells in vitro and in vivo.
title_full	Nocardia brasiliensis induces formation of foamy macrophages and dendritic cells in vitro and in vivo.
title_fullStr	Nocardia brasiliensis induces formation of foamy macrophages and dendritic cells in vitro and in vivo.
title_full_unstemmed	Nocardia brasiliensis induces formation of foamy macrophages and dendritic cells in vitro and in vivo.
title_sort	nocardia brasiliensis induces formation of foamy macrophages and dendritic cells in vitro and in vivo.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/de026d494eb14a9bab0265804346ad7b
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Nocardia brasiliensis induces formation of foamy macrophages and dendritic cells in vitro and in vivo.

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