Experimental Infection of voles with Francisella tularensis indicates their amplification role in tularemia outbreaks.

Tularemia outbreaks in humans have been linked to fluctuations in rodent population density, but the mode of bacterial maintenance in nature is unclear. Here we report on an experiment to investigate the pathogenesis of Francisella tularensis infection in wild rodents, and thereby assess their poten...

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Autores principales:	Heidi Rossow, Kristian M Forbes, Eveliina Tarkka, Paula M Kinnunen, Heidi Hemmilä, Otso Huitu, Simo Nikkari, Heikki Henttonen, Anja Kipar, Olli Vapalahti
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/de03f1f611314be5b8d184071be5f6ae
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spelling	oai:doaj.org-article:de03f1f611314be5b8d184071be5f6ae2021-11-25T05:58:24ZExperimental Infection of voles with Francisella tularensis indicates their amplification role in tularemia outbreaks.1932-620310.1371/journal.pone.0108864https://doaj.org/article/de03f1f611314be5b8d184071be5f6ae2014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0108864https://doaj.org/toc/1932-6203Tularemia outbreaks in humans have been linked to fluctuations in rodent population density, but the mode of bacterial maintenance in nature is unclear. Here we report on an experiment to investigate the pathogenesis of Francisella tularensis infection in wild rodents, and thereby assess their potential to spread the bacterium. We infected 20 field voles (Microtus agrestis) and 12 bank voles (Myodes glareolus) with a strain of F. tularensis ssp. holarctica isolated from a human patient. Upon euthanasia or death, voles were necropsied and specimens collected for histological assessment and identification of bacteria by immunohistology and PCR. Bacterial excretion and a rapid lethal clinical course with pathological changes consistent with bacteremia and tissue necrosis were observed in infected animals. The results support a role for voles as an amplification host of F. tularensis, as excreta and, in particular, carcasses with high bacterial burden could serve as a source for environmental contamination.Heidi RossowKristian M ForbesEveliina TarkkaPaula M KinnunenHeidi HemmiläOtso HuituSimo NikkariHeikki HenttonenAnja KiparOlli VapalahtiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 10, p e108864 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Heidi Rossow Kristian M Forbes Eveliina Tarkka Paula M Kinnunen Heidi Hemmilä Otso Huitu Simo Nikkari Heikki Henttonen Anja Kipar Olli Vapalahti Experimental Infection of voles with Francisella tularensis indicates their amplification role in tularemia outbreaks.
description	Tularemia outbreaks in humans have been linked to fluctuations in rodent population density, but the mode of bacterial maintenance in nature is unclear. Here we report on an experiment to investigate the pathogenesis of Francisella tularensis infection in wild rodents, and thereby assess their potential to spread the bacterium. We infected 20 field voles (Microtus agrestis) and 12 bank voles (Myodes glareolus) with a strain of F. tularensis ssp. holarctica isolated from a human patient. Upon euthanasia or death, voles were necropsied and specimens collected for histological assessment and identification of bacteria by immunohistology and PCR. Bacterial excretion and a rapid lethal clinical course with pathological changes consistent with bacteremia and tissue necrosis were observed in infected animals. The results support a role for voles as an amplification host of F. tularensis, as excreta and, in particular, carcasses with high bacterial burden could serve as a source for environmental contamination.
format	article
author	Heidi Rossow Kristian M Forbes Eveliina Tarkka Paula M Kinnunen Heidi Hemmilä Otso Huitu Simo Nikkari Heikki Henttonen Anja Kipar Olli Vapalahti
author_facet	Heidi Rossow Kristian M Forbes Eveliina Tarkka Paula M Kinnunen Heidi Hemmilä Otso Huitu Simo Nikkari Heikki Henttonen Anja Kipar Olli Vapalahti
author_sort	Heidi Rossow
title	Experimental Infection of voles with Francisella tularensis indicates their amplification role in tularemia outbreaks.
title_short	Experimental Infection of voles with Francisella tularensis indicates their amplification role in tularemia outbreaks.
title_full	Experimental Infection of voles with Francisella tularensis indicates their amplification role in tularemia outbreaks.
title_fullStr	Experimental Infection of voles with Francisella tularensis indicates their amplification role in tularemia outbreaks.
title_full_unstemmed	Experimental Infection of voles with Francisella tularensis indicates their amplification role in tularemia outbreaks.
title_sort	experimental infection of voles with francisella tularensis indicates their amplification role in tularemia outbreaks.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/de03f1f611314be5b8d184071be5f6ae
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Experimental Infection of voles with Francisella tularensis indicates their amplification role in tularemia outbreaks.

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