DETERMINING REFERENCE RANGES FOR TREC AND KREC ASSAYS IN IMMUNE DEFICIENCY SCREENING OF NEWBORNS IN RUSSIAN FEDERATION

In this work, we used a reference population of newborns and sampled dried blood spots on Guthrie cards of 2,739 individual samples to determine the reference intervals for TRECs and KRECs values, in order to diagnose primary immunodeficiency by means of neonatal screening. The median absolute value...

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Autores principales:	M. A. Gordukova, I. A. Korsunsky, Yu. V. Chursinova, M. M. Byakhova, I. P. Oscorbin, A. P. Prodeus, M. L. Filipenko
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2019
Materias:	primary immunodeficiency (pid) t cells b cells screening trec krec newborn reference intervals Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/de1469d591de4702887675e2b93ca48c
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spelling	oai:doaj.org-article:de1469d591de4702887675e2b93ca48c2021-11-18T08:03:48ZDETERMINING REFERENCE RANGES FOR TREC AND KREC ASSAYS IN IMMUNE DEFICIENCY SCREENING OF NEWBORNS IN RUSSIAN FEDERATION1563-06252313-741X10.15789/1563-0625-2019-3-527-538https://doaj.org/article/de1469d591de4702887675e2b93ca48c2019-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1826https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XIn this work, we used a reference population of newborns and sampled dried blood spots on Guthrie cards of 2,739 individual samples to determine the reference intervals for TRECs and KRECs values, in order to diagnose primary immunodeficiency by means of neonatal screening. The median absolute values for TRECs and KRECs were 195 (CI95%: 185-206) and 185 (CI95%: 176-197) copies per μl, respectively; the normalized value for TRECs was 2780 (CI95%: 2690-2840), and for KRECs, 2790 (CI95%: 2700-2900) copies per 2 × 105 copies of the albumin gene or 105 cells. The reference interval was calculated for 99 and 99.9 percentiles of total TRECs and KRECs individual values. Due to asymmetric distribution of data, the outliers were filtered off, using the Tukey’s criterion applied after logarithmic transformation of the data. When analyzing absolute values for TREC/KREC (per μL of blood), no “drop-down” TRECs values were identified; for KRECs, 18 experimental values were excluded from further analysis (from 9.8 to 13.5). The outlying values were not identified among the normalized values of TRECs/KRECs. The obtained reference values for TRECs and KRECs (at the 0.1 percentile level) were, respectively, 458 and 32 per 105 cells, or 23 and 17 per μl of blood samples from neonates.M. A. GordukovaI. A. KorsunskyYu. V. ChursinovaM. M. ByakhovaI. P. OscorbinA. P. ProdeusM. L. FilipenkoSPb RAACIarticleprimary immunodeficiency (pid)t cellsb cellsscreeningtreckrecnewbornreference intervalsImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 21, Iss 3, Pp 527-538 (2019)
institution	DOAJ
collection	DOAJ
language	RU
topic	primary immunodeficiency (pid) t cells b cells screening trec krec newborn reference intervals Immunologic diseases. Allergy RC581-607
spellingShingle	primary immunodeficiency (pid) t cells b cells screening trec krec newborn reference intervals Immunologic diseases. Allergy RC581-607 M. A. Gordukova I. A. Korsunsky Yu. V. Chursinova M. M. Byakhova I. P. Oscorbin A. P. Prodeus M. L. Filipenko DETERMINING REFERENCE RANGES FOR TREC AND KREC ASSAYS IN IMMUNE DEFICIENCY SCREENING OF NEWBORNS IN RUSSIAN FEDERATION
description	In this work, we used a reference population of newborns and sampled dried blood spots on Guthrie cards of 2,739 individual samples to determine the reference intervals for TRECs and KRECs values, in order to diagnose primary immunodeficiency by means of neonatal screening. The median absolute values for TRECs and KRECs were 195 (CI95%: 185-206) and 185 (CI95%: 176-197) copies per μl, respectively; the normalized value for TRECs was 2780 (CI95%: 2690-2840), and for KRECs, 2790 (CI95%: 2700-2900) copies per 2 × 105 copies of the albumin gene or 105 cells. The reference interval was calculated for 99 and 99.9 percentiles of total TRECs and KRECs individual values. Due to asymmetric distribution of data, the outliers were filtered off, using the Tukey’s criterion applied after logarithmic transformation of the data. When analyzing absolute values for TREC/KREC (per μL of blood), no “drop-down” TRECs values were identified; for KRECs, 18 experimental values were excluded from further analysis (from 9.8 to 13.5). The outlying values were not identified among the normalized values of TRECs/KRECs. The obtained reference values for TRECs and KRECs (at the 0.1 percentile level) were, respectively, 458 and 32 per 105 cells, or 23 and 17 per μl of blood samples from neonates.
format	article
author	M. A. Gordukova I. A. Korsunsky Yu. V. Chursinova M. M. Byakhova I. P. Oscorbin A. P. Prodeus M. L. Filipenko
author_facet	M. A. Gordukova I. A. Korsunsky Yu. V. Chursinova M. M. Byakhova I. P. Oscorbin A. P. Prodeus M. L. Filipenko
author_sort	M. A. Gordukova
title	DETERMINING REFERENCE RANGES FOR TREC AND KREC ASSAYS IN IMMUNE DEFICIENCY SCREENING OF NEWBORNS IN RUSSIAN FEDERATION
title_short	DETERMINING REFERENCE RANGES FOR TREC AND KREC ASSAYS IN IMMUNE DEFICIENCY SCREENING OF NEWBORNS IN RUSSIAN FEDERATION
title_full	DETERMINING REFERENCE RANGES FOR TREC AND KREC ASSAYS IN IMMUNE DEFICIENCY SCREENING OF NEWBORNS IN RUSSIAN FEDERATION
title_fullStr	DETERMINING REFERENCE RANGES FOR TREC AND KREC ASSAYS IN IMMUNE DEFICIENCY SCREENING OF NEWBORNS IN RUSSIAN FEDERATION
title_full_unstemmed	DETERMINING REFERENCE RANGES FOR TREC AND KREC ASSAYS IN IMMUNE DEFICIENCY SCREENING OF NEWBORNS IN RUSSIAN FEDERATION
title_sort	determining reference ranges for trec and krec assays in immune deficiency screening of newborns in russian federation
publisher	SPb RAACI
publishDate	2019
url	https://doaj.org/article/de1469d591de4702887675e2b93ca48c
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DETERMINING REFERENCE RANGES FOR TREC AND KREC ASSAYS IN IMMUNE DEFICIENCY SCREENING OF NEWBORNS IN RUSSIAN FEDERATION

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