Sensitive Colorimetric Detection of Interleukin-6 via Lateral Flow Assay Incorporated Silver Amplification Method

Interleukin-6 (IL-6) is a pro/anti-inflammatory cytokine, the quantitative detection of which has been extensively considered for diagnosis of inflammatory associated diseases. However, there has not yet been a reliable, low-cost, and user-friendly platform developed for point-of-care (POC) detectio...

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Autores principales:	Mohammad Rahbar, Yuling Wu, J. Anand Subramony, Guozhen Liu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	point-of-care diagnostics lateral flow immunoassay interleukin-6 silver enhancement reagent paper test strips colorimetric detection Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/de25c03fa2fc4e6a904e4c80afd7cd3b
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spelling	oai:doaj.org-article:de25c03fa2fc4e6a904e4c80afd7cd3b2021-12-01T08:34:57ZSensitive Colorimetric Detection of Interleukin-6 via Lateral Flow Assay Incorporated Silver Amplification Method2296-418510.3389/fbioe.2021.778269https://doaj.org/article/de25c03fa2fc4e6a904e4c80afd7cd3b2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.778269/fullhttps://doaj.org/toc/2296-4185Interleukin-6 (IL-6) is a pro/anti-inflammatory cytokine, the quantitative detection of which has been extensively considered for diagnosis of inflammatory associated diseases. However, there has not yet been a reliable, low-cost, and user-friendly platform developed for point-of-care (POC) detection of IL-6, which will eliminate the conventional costly, time-consuming, and complex assays. In this work, we developed a lateral flow assay for colorimetric detection of IL-6, using anti-IL-6 antibodies conjugated to gold nanoparticles (AuNPs) as the detection probes. Silver amplification technique was incorporated with the newly developed assay in order to enhance the obtained colorimetric signals, allowing sensitive detection of IL-6 in human serum in the desired physiological ranges (i.e., 5–1000 pg/mL). A limit of detection of 5 pg/mL could be achieved for IL-6 detection in serum with the amplification step which was not achievable in the standard assay. The corresponding specificity and reproducibility tests were all preformed to confirm the reliability of this assay for quantitative measurement of IL-6 in a POC manner.Mohammad RahbarYuling WuJ. Anand SubramonyGuozhen LiuGuozhen LiuFrontiers Media S.A.articlepoint-of-care diagnosticslateral flow immunoassayinterleukin-6silver enhancement reagentpaper test stripscolorimetric detectionBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	point-of-care diagnostics lateral flow immunoassay interleukin-6 silver enhancement reagent paper test strips colorimetric detection Biotechnology TP248.13-248.65
spellingShingle	point-of-care diagnostics lateral flow immunoassay interleukin-6 silver enhancement reagent paper test strips colorimetric detection Biotechnology TP248.13-248.65 Mohammad Rahbar Yuling Wu J. Anand Subramony Guozhen Liu Guozhen Liu Sensitive Colorimetric Detection of Interleukin-6 via Lateral Flow Assay Incorporated Silver Amplification Method
description	Interleukin-6 (IL-6) is a pro/anti-inflammatory cytokine, the quantitative detection of which has been extensively considered for diagnosis of inflammatory associated diseases. However, there has not yet been a reliable, low-cost, and user-friendly platform developed for point-of-care (POC) detection of IL-6, which will eliminate the conventional costly, time-consuming, and complex assays. In this work, we developed a lateral flow assay for colorimetric detection of IL-6, using anti-IL-6 antibodies conjugated to gold nanoparticles (AuNPs) as the detection probes. Silver amplification technique was incorporated with the newly developed assay in order to enhance the obtained colorimetric signals, allowing sensitive detection of IL-6 in human serum in the desired physiological ranges (i.e., 5–1000 pg/mL). A limit of detection of 5 pg/mL could be achieved for IL-6 detection in serum with the amplification step which was not achievable in the standard assay. The corresponding specificity and reproducibility tests were all preformed to confirm the reliability of this assay for quantitative measurement of IL-6 in a POC manner.
format	article
author	Mohammad Rahbar Yuling Wu J. Anand Subramony Guozhen Liu Guozhen Liu
author_facet	Mohammad Rahbar Yuling Wu J. Anand Subramony Guozhen Liu Guozhen Liu
author_sort	Mohammad Rahbar
title	Sensitive Colorimetric Detection of Interleukin-6 via Lateral Flow Assay Incorporated Silver Amplification Method
title_short	Sensitive Colorimetric Detection of Interleukin-6 via Lateral Flow Assay Incorporated Silver Amplification Method
title_full	Sensitive Colorimetric Detection of Interleukin-6 via Lateral Flow Assay Incorporated Silver Amplification Method
title_fullStr	Sensitive Colorimetric Detection of Interleukin-6 via Lateral Flow Assay Incorporated Silver Amplification Method
title_full_unstemmed	Sensitive Colorimetric Detection of Interleukin-6 via Lateral Flow Assay Incorporated Silver Amplification Method
title_sort	sensitive colorimetric detection of interleukin-6 via lateral flow assay incorporated silver amplification method
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/de25c03fa2fc4e6a904e4c80afd7cd3b
work_keys_str_mv	AT mohammadrahbar sensitivecolorimetricdetectionofinterleukin6vialateralflowassayincorporatedsilveramplificationmethod AT yulingwu sensitivecolorimetricdetectionofinterleukin6vialateralflowassayincorporatedsilveramplificationmethod AT janandsubramony sensitivecolorimetricdetectionofinterleukin6vialateralflowassayincorporatedsilveramplificationmethod AT guozhenliu sensitivecolorimetricdetectionofinterleukin6vialateralflowassayincorporatedsilveramplificationmethod AT guozhenliu sensitivecolorimetricdetectionofinterleukin6vialateralflowassayincorporatedsilveramplificationmethod
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Sensitive Colorimetric Detection of Interleukin-6 via Lateral Flow Assay Incorporated Silver Amplification Method

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