PIGN spatiotemporally regulates the spindle assembly checkpoint proteins in leukemia transformation and progression

Abstract Phosphatidylinositol glycan anchor biosynthesis class N (PIGN) has been linked to the suppression of chromosomal instability. The spindle assembly checkpoint complex is responsible for proper chromosome segregation during mitosis to prevent chromosomal instability. In this study, the novel...

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Autores principales:	Emmanuel K. Teye, Shasha Lu, Fangyuan Chen, Wenrui Yang, Thomas Abraham, Douglas B. Stairs, Hong-Gang Wang, Gregory S. Yochum, Robert A. Brodsky, Jeffrey J. Pu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/de2cadc6ed4e4474b1a7633bdd7d1cd8
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spelling	oai:doaj.org-article:de2cadc6ed4e4474b1a7633bdd7d1cd82021-12-02T15:14:48ZPIGN spatiotemporally regulates the spindle assembly checkpoint proteins in leukemia transformation and progression10.1038/s41598-021-98218-y2045-2322https://doaj.org/article/de2cadc6ed4e4474b1a7633bdd7d1cd82021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98218-yhttps://doaj.org/toc/2045-2322Abstract Phosphatidylinositol glycan anchor biosynthesis class N (PIGN) has been linked to the suppression of chromosomal instability. The spindle assembly checkpoint complex is responsible for proper chromosome segregation during mitosis to prevent chromosomal instability. In this study, the novel role of PIGN as a regulator of the spindle assembly checkpoint was unveiled in leukemic patient cells and cell lines. Transient downregulation or ablation of PIGN resulted in impaired mitotic checkpoint activation due to the dysregulated expression of spindle assembly checkpoint-related proteins including MAD1, MAD2, BUBR1, and MPS1. Moreover, ectopic overexpression of PIGN restored the expression of MAD2. PIGN regulated the spindle assembly checkpoint by forming a complex with the spindle assembly checkpoint proteins MAD1, MAD2, and the mitotic kinase MPS1. Thus, PIGN could play a vital role in the spindle assembly checkpoint to suppress chromosomal instability associated with leukemic transformation and progression.Emmanuel K. TeyeShasha LuFangyuan ChenWenrui YangThomas AbrahamDouglas B. StairsHong-Gang WangGregory S. YochumRobert A. BrodskyJeffrey J. PuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Emmanuel K. Teye Shasha Lu Fangyuan Chen Wenrui Yang Thomas Abraham Douglas B. Stairs Hong-Gang Wang Gregory S. Yochum Robert A. Brodsky Jeffrey J. Pu PIGN spatiotemporally regulates the spindle assembly checkpoint proteins in leukemia transformation and progression
description	Abstract Phosphatidylinositol glycan anchor biosynthesis class N (PIGN) has been linked to the suppression of chromosomal instability. The spindle assembly checkpoint complex is responsible for proper chromosome segregation during mitosis to prevent chromosomal instability. In this study, the novel role of PIGN as a regulator of the spindle assembly checkpoint was unveiled in leukemic patient cells and cell lines. Transient downregulation or ablation of PIGN resulted in impaired mitotic checkpoint activation due to the dysregulated expression of spindle assembly checkpoint-related proteins including MAD1, MAD2, BUBR1, and MPS1. Moreover, ectopic overexpression of PIGN restored the expression of MAD2. PIGN regulated the spindle assembly checkpoint by forming a complex with the spindle assembly checkpoint proteins MAD1, MAD2, and the mitotic kinase MPS1. Thus, PIGN could play a vital role in the spindle assembly checkpoint to suppress chromosomal instability associated with leukemic transformation and progression.
format	article
author	Emmanuel K. Teye Shasha Lu Fangyuan Chen Wenrui Yang Thomas Abraham Douglas B. Stairs Hong-Gang Wang Gregory S. Yochum Robert A. Brodsky Jeffrey J. Pu
author_facet	Emmanuel K. Teye Shasha Lu Fangyuan Chen Wenrui Yang Thomas Abraham Douglas B. Stairs Hong-Gang Wang Gregory S. Yochum Robert A. Brodsky Jeffrey J. Pu
author_sort	Emmanuel K. Teye
title	PIGN spatiotemporally regulates the spindle assembly checkpoint proteins in leukemia transformation and progression
title_short	PIGN spatiotemporally regulates the spindle assembly checkpoint proteins in leukemia transformation and progression
title_full	PIGN spatiotemporally regulates the spindle assembly checkpoint proteins in leukemia transformation and progression
title_fullStr	PIGN spatiotemporally regulates the spindle assembly checkpoint proteins in leukemia transformation and progression
title_full_unstemmed	PIGN spatiotemporally regulates the spindle assembly checkpoint proteins in leukemia transformation and progression
title_sort	pign spatiotemporally regulates the spindle assembly checkpoint proteins in leukemia transformation and progression
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/de2cadc6ed4e4474b1a7633bdd7d1cd8
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PIGN spatiotemporally regulates the spindle assembly checkpoint proteins in leukemia transformation and progression

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