Effect of Trichoderma harzianum on maize rhizosphere microbiome and biocontrol of Fusarium Stalk rot

Abstract Fusarium stalk rot (FSR) caused by Fusarium graminearum (FG) significantly affects the productivity of maize grain crops. Application of agrochemicals to control the disease is harmful to environment. In this regard, use of biocontrol agent (BCA) is an alternative to agrochemicals. Although...

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Autores principales:	Kandasamy Saravanakumar, Yaqian Li, Chuanjin Yu, Qiang-qiang Wang, Meng Wang, Jianan Sun, Jin-xin Gao, Jie Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/de3570c273b24201bae2f6a85b15976f
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spelling	oai:doaj.org-article:de3570c273b24201bae2f6a85b15976f2021-12-02T12:32:50ZEffect of Trichoderma harzianum on maize rhizosphere microbiome and biocontrol of Fusarium Stalk rot10.1038/s41598-017-01680-w2045-2322https://doaj.org/article/de3570c273b24201bae2f6a85b15976f2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01680-whttps://doaj.org/toc/2045-2322Abstract Fusarium stalk rot (FSR) caused by Fusarium graminearum (FG) significantly affects the productivity of maize grain crops. Application of agrochemicals to control the disease is harmful to environment. In this regard, use of biocontrol agent (BCA) is an alternative to agrochemicals. Although Trichoderma species are known as BCA, the selection of host-pathogen specific Trichoderma is essential for the successful field application. Hence, we screened a total of 100 Trichoderma isolates against FG, selected Trichoderma harzianum (CCTCC-RW0024) for greenhouse experiments and studied its effect on changes of maize rhizosphere microbiome and biocontrol of FSR. The strain CCTCC-RW0024 displayed high antagonistic activity (96.30%), disease reduction (86.66%), biocontrol-related enzyme and gene expression. The root colonization of the strain was confirmed by eGFP tagging and qRT-PCR analysis. Pyrosequencing revealed that exogenous inoculation of the strain in maize rhizosphere increased the plant growth promoting acidobacteria (18.4%), decreased 66% of FG, and also increased the plant growth. In addition, metabolites of this strain could interact with pathogenicity related transcriptional cofactor FgSWi6, thereby contributing to its inhibition. It is concluded that T. harzianum strain CCTCC-RW0024 is a potential BCA against FSR.Kandasamy SaravanakumarYaqian LiChuanjin YuQiang-qiang WangMeng WangJianan SunJin-xin GaoJie ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kandasamy Saravanakumar Yaqian Li Chuanjin Yu Qiang-qiang Wang Meng Wang Jianan Sun Jin-xin Gao Jie Chen Effect of Trichoderma harzianum on maize rhizosphere microbiome and biocontrol of Fusarium Stalk rot
description	Abstract Fusarium stalk rot (FSR) caused by Fusarium graminearum (FG) significantly affects the productivity of maize grain crops. Application of agrochemicals to control the disease is harmful to environment. In this regard, use of biocontrol agent (BCA) is an alternative to agrochemicals. Although Trichoderma species are known as BCA, the selection of host-pathogen specific Trichoderma is essential for the successful field application. Hence, we screened a total of 100 Trichoderma isolates against FG, selected Trichoderma harzianum (CCTCC-RW0024) for greenhouse experiments and studied its effect on changes of maize rhizosphere microbiome and biocontrol of FSR. The strain CCTCC-RW0024 displayed high antagonistic activity (96.30%), disease reduction (86.66%), biocontrol-related enzyme and gene expression. The root colonization of the strain was confirmed by eGFP tagging and qRT-PCR analysis. Pyrosequencing revealed that exogenous inoculation of the strain in maize rhizosphere increased the plant growth promoting acidobacteria (18.4%), decreased 66% of FG, and also increased the plant growth. In addition, metabolites of this strain could interact with pathogenicity related transcriptional cofactor FgSWi6, thereby contributing to its inhibition. It is concluded that T. harzianum strain CCTCC-RW0024 is a potential BCA against FSR.
format	article
author	Kandasamy Saravanakumar Yaqian Li Chuanjin Yu Qiang-qiang Wang Meng Wang Jianan Sun Jin-xin Gao Jie Chen
author_facet	Kandasamy Saravanakumar Yaqian Li Chuanjin Yu Qiang-qiang Wang Meng Wang Jianan Sun Jin-xin Gao Jie Chen
author_sort	Kandasamy Saravanakumar
title	Effect of Trichoderma harzianum on maize rhizosphere microbiome and biocontrol of Fusarium Stalk rot
title_short	Effect of Trichoderma harzianum on maize rhizosphere microbiome and biocontrol of Fusarium Stalk rot
title_full	Effect of Trichoderma harzianum on maize rhizosphere microbiome and biocontrol of Fusarium Stalk rot
title_fullStr	Effect of Trichoderma harzianum on maize rhizosphere microbiome and biocontrol of Fusarium Stalk rot
title_full_unstemmed	Effect of Trichoderma harzianum on maize rhizosphere microbiome and biocontrol of Fusarium Stalk rot
title_sort	effect of trichoderma harzianum on maize rhizosphere microbiome and biocontrol of fusarium stalk rot
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/de3570c273b24201bae2f6a85b15976f
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Effect of Trichoderma harzianum on maize rhizosphere microbiome and biocontrol of Fusarium Stalk rot

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