Genomic diversity of bacteriophages infecting Rhodobacter capsulatus and their relatedness to its gene transfer agent RcGTA.

The diversity of bacteriophages is likely unparalleled in the biome due to the immense variety of hosts and the multitude of viruses that infect them. Recent efforts have led to description at the genomic level of numerous bacteriophages that infect the Actinobacteria, but relatively little is known...

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Autores principales:	Jackson Rapala, Brenda Miller, Maximiliano Garcia, Megan Dolan, Matthew Bockman, Mats Hansson, Daniel A Russell, Rebecca A Garlena, Steven G Cresawn, Alexander B Westbye, J Thomas Beatty, Richard M Alvey, David W Bollivar
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/de3989e8aeec470c8b3157db910ac921
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spelling	oai:doaj.org-article:de3989e8aeec470c8b3157db910ac9212021-12-02T20:12:51ZGenomic diversity of bacteriophages infecting Rhodobacter capsulatus and their relatedness to its gene transfer agent RcGTA.1932-620310.1371/journal.pone.0255262https://doaj.org/article/de3989e8aeec470c8b3157db910ac9212021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255262https://doaj.org/toc/1932-6203The diversity of bacteriophages is likely unparalleled in the biome due to the immense variety of hosts and the multitude of viruses that infect them. Recent efforts have led to description at the genomic level of numerous bacteriophages that infect the Actinobacteria, but relatively little is known about those infecting other prokaryotic phyla, such as the purple non-sulfur photosynthetic α-proteobacterium Rhodobacter capsulatus. This species is a common inhabitant of freshwater ecosystems and has been an important model system for the study of photosynthesis. Additionally, it is notable for its utilization of a unique form of horizontal gene transfer via a bacteriophage-like element known as the gene transfer agent (RcGTA). Only three bacteriophages of R. capsulatus had been sequenced prior to this report. Isolation and characterization at the genomic level of 26 new bacteriophages infecting this host advances the understanding of bacteriophage diversity and the origins of RcGTA. These newly discovered isolates can be grouped along with three that were previously sequenced to form six clusters with four remaining as single representatives. These bacteriophages share genes with RcGTA that seem to be related to host recognition. One isolate was found to cause lysis of a marine bacterium when exposed to high-titer lysate. Although some clusters are more highly represented in the sequenced genomes, it is evident that many more bacteriophage types that infect R. capsulatus are likely to be found in the future.Jackson RapalaBrenda MillerMaximiliano GarciaMegan DolanMatthew BockmanMats HanssonDaniel A RussellRebecca A GarlenaSteven G CresawnAlexander B WestbyeJ Thomas BeattyRichard M AlveyDavid W BollivarPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11, p e0255262 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jackson Rapala Brenda Miller Maximiliano Garcia Megan Dolan Matthew Bockman Mats Hansson Daniel A Russell Rebecca A Garlena Steven G Cresawn Alexander B Westbye J Thomas Beatty Richard M Alvey David W Bollivar Genomic diversity of bacteriophages infecting Rhodobacter capsulatus and their relatedness to its gene transfer agent RcGTA.
description	The diversity of bacteriophages is likely unparalleled in the biome due to the immense variety of hosts and the multitude of viruses that infect them. Recent efforts have led to description at the genomic level of numerous bacteriophages that infect the Actinobacteria, but relatively little is known about those infecting other prokaryotic phyla, such as the purple non-sulfur photosynthetic α-proteobacterium Rhodobacter capsulatus. This species is a common inhabitant of freshwater ecosystems and has been an important model system for the study of photosynthesis. Additionally, it is notable for its utilization of a unique form of horizontal gene transfer via a bacteriophage-like element known as the gene transfer agent (RcGTA). Only three bacteriophages of R. capsulatus had been sequenced prior to this report. Isolation and characterization at the genomic level of 26 new bacteriophages infecting this host advances the understanding of bacteriophage diversity and the origins of RcGTA. These newly discovered isolates can be grouped along with three that were previously sequenced to form six clusters with four remaining as single representatives. These bacteriophages share genes with RcGTA that seem to be related to host recognition. One isolate was found to cause lysis of a marine bacterium when exposed to high-titer lysate. Although some clusters are more highly represented in the sequenced genomes, it is evident that many more bacteriophage types that infect R. capsulatus are likely to be found in the future.
format	article
author	Jackson Rapala Brenda Miller Maximiliano Garcia Megan Dolan Matthew Bockman Mats Hansson Daniel A Russell Rebecca A Garlena Steven G Cresawn Alexander B Westbye J Thomas Beatty Richard M Alvey David W Bollivar
author_facet	Jackson Rapala Brenda Miller Maximiliano Garcia Megan Dolan Matthew Bockman Mats Hansson Daniel A Russell Rebecca A Garlena Steven G Cresawn Alexander B Westbye J Thomas Beatty Richard M Alvey David W Bollivar
author_sort	Jackson Rapala
title	Genomic diversity of bacteriophages infecting Rhodobacter capsulatus and their relatedness to its gene transfer agent RcGTA.
title_short	Genomic diversity of bacteriophages infecting Rhodobacter capsulatus and their relatedness to its gene transfer agent RcGTA.
title_full	Genomic diversity of bacteriophages infecting Rhodobacter capsulatus and their relatedness to its gene transfer agent RcGTA.
title_fullStr	Genomic diversity of bacteriophages infecting Rhodobacter capsulatus and their relatedness to its gene transfer agent RcGTA.
title_full_unstemmed	Genomic diversity of bacteriophages infecting Rhodobacter capsulatus and their relatedness to its gene transfer agent RcGTA.
title_sort	genomic diversity of bacteriophages infecting rhodobacter capsulatus and their relatedness to its gene transfer agent rcgta.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/de3989e8aeec470c8b3157db910ac921
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Genomic diversity of bacteriophages infecting Rhodobacter capsulatus and their relatedness to its gene transfer agent RcGTA.

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