Studies on Genetic Divergence in Pomegranate (Punica granatum L.) Using SRAP Markers
Pomegranate genotypes have been characterized mainly on the basis of morphological traits; but, these traits are affected to a large extent by environmental and cultivation conditions, resulting in their ambiguous discrimination. Molecular markers are more suited for accurate discrimination of genot...
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Society for Promotion of Horticulture - Indian Institute of Horticultural Research
2015
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oai:doaj.org-article:de3c6e1796464b0386e2cf96406203e42021-12-02T11:14:40ZStudies on Genetic Divergence in Pomegranate (Punica granatum L.) Using SRAP Markers0973-354X2582-4899https://doaj.org/article/de3c6e1796464b0386e2cf96406203e42015-12-01T00:00:00Zhttps://jhs.iihr.res.in/index.php/jhs/article/view/116https://doaj.org/toc/0973-354Xhttps://doaj.org/toc/2582-4899Pomegranate genotypes have been characterized mainly on the basis of morphological traits; but, these traits are affected to a large extent by environmental and cultivation conditions, resulting in their ambiguous discrimination. Molecular markers are more suited for accurate discrimination of genotypes and cultivars. Sequence-Related Amplified Polymorphism (SRAP) markers were used in the present study to analyze polymorphism among the important pomegranate genotypes grown in India. The total number of bands generated by 30 SRAP primers for 12 genotypes was 1448, with an average of 48.3 bands per primer. Polymorphism varied from 2.7 to 73.9, with an average of 40.95%. Similarity-value based on Jaccard's Coefficient ranged from 0.63 (between cvs. Naina and Amlidana) to 0.95 (between cvs. Kabul Yellow and Jalore Seedless). UPGMA (un-weighted pair group method with arithmetic mean) analysis was performed and a dendrogram was constructed using Jaccard's similarity matrix. The 12 genotypes used grouped into 5 clusters. SRAP markers were found suitable for determining variability among the pomegranate genotypes studied.C KanupriyaD Manmohan KumarP NischitaM GayathriK V RavishankarP Sampath KumarSociety for Promotion of Horticulture - Indian Institute of Horticultural Researcharticlepomegranatemolecular markerssrapgenetic diversityPlant cultureSB1-1110ENJournal of Horticultural Sciences, Vol 10, Iss 2, Pp 125-129 (2015) |
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pomegranate molecular markers srap genetic diversity Plant culture SB1-1110 |
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pomegranate molecular markers srap genetic diversity Plant culture SB1-1110 C Kanupriya D Manmohan Kumar P Nischita M Gayathri K V Ravishankar P Sampath Kumar Studies on Genetic Divergence in Pomegranate (Punica granatum L.) Using SRAP Markers |
description |
Pomegranate genotypes have been characterized mainly on the basis of morphological traits; but, these traits are affected to a large extent by environmental and cultivation conditions, resulting in their ambiguous discrimination. Molecular markers are more suited for accurate discrimination of genotypes and cultivars. Sequence-Related Amplified Polymorphism (SRAP) markers were used in the present study to analyze polymorphism among the important pomegranate genotypes grown in India. The total number of bands generated by 30 SRAP primers for 12 genotypes was 1448, with an average of 48.3 bands per primer. Polymorphism varied from 2.7 to 73.9, with an average of 40.95%. Similarity-value based on Jaccard's Coefficient ranged from 0.63 (between cvs. Naina and Amlidana) to 0.95 (between cvs. Kabul Yellow and Jalore Seedless). UPGMA (un-weighted pair group method with arithmetic mean) analysis was performed and a dendrogram was constructed using Jaccard's similarity matrix. The 12 genotypes used grouped into 5 clusters. SRAP markers were found suitable for determining variability among the pomegranate genotypes studied. |
format |
article |
author |
C Kanupriya D Manmohan Kumar P Nischita M Gayathri K V Ravishankar P Sampath Kumar |
author_facet |
C Kanupriya D Manmohan Kumar P Nischita M Gayathri K V Ravishankar P Sampath Kumar |
author_sort |
C Kanupriya |
title |
Studies on Genetic Divergence in Pomegranate (Punica granatum L.) Using SRAP Markers |
title_short |
Studies on Genetic Divergence in Pomegranate (Punica granatum L.) Using SRAP Markers |
title_full |
Studies on Genetic Divergence in Pomegranate (Punica granatum L.) Using SRAP Markers |
title_fullStr |
Studies on Genetic Divergence in Pomegranate (Punica granatum L.) Using SRAP Markers |
title_full_unstemmed |
Studies on Genetic Divergence in Pomegranate (Punica granatum L.) Using SRAP Markers |
title_sort |
studies on genetic divergence in pomegranate (punica granatum l.) using srap markers |
publisher |
Society for Promotion of Horticulture - Indian Institute of Horticultural Research |
publishDate |
2015 |
url |
https://doaj.org/article/de3c6e1796464b0386e2cf96406203e4 |
work_keys_str_mv |
AT ckanupriya studiesongeneticdivergenceinpomegranatepunicagranatumlusingsrapmarkers AT dmanmohankumar studiesongeneticdivergenceinpomegranatepunicagranatumlusingsrapmarkers AT pnischita studiesongeneticdivergenceinpomegranatepunicagranatumlusingsrapmarkers AT mgayathri studiesongeneticdivergenceinpomegranatepunicagranatumlusingsrapmarkers AT kvravishankar studiesongeneticdivergenceinpomegranatepunicagranatumlusingsrapmarkers AT psampathkumar studiesongeneticdivergenceinpomegranatepunicagranatumlusingsrapmarkers |
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