SIRT1 Promotes Osteogenic Differentiation in Human Dental Pulp Stem Cells through Counteracting the Activation of STAT3

Human dental pulp stem cells (hDPSCs), which are characterized by self-renewal capacity and the ability of multilineage differentiation, have gained increased attention in regenerative medicine recently. Histone acetylation modulator proteins (HAMPs) are a protein family that mediates the modificati...

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Autores principales:	Dan Zhao, Wen Kang, Yiwen Wang, Jiuyu Ge, Jianfeng Huang, Jie Yang, Weidong Yang, Xuna Tang, Sijing Xie
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	dental pulp stem cells SIRT1 STAT3 histone acetylation modulator proteins osteogenic differentiation Engineering (General). Civil engineering (General) TA1-2040
Acceso en línea:	https://doaj.org/article/de40f411780a4a4780893765f509c150
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spelling	oai:doaj.org-article:de40f411780a4a4780893765f509c1502021-11-25T17:16:23ZSIRT1 Promotes Osteogenic Differentiation in Human Dental Pulp Stem Cells through Counteracting the Activation of STAT310.3390/coatings111113532079-6412https://doaj.org/article/de40f411780a4a4780893765f509c1502021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6412/11/11/1353https://doaj.org/toc/2079-6412Human dental pulp stem cells (hDPSCs), which are characterized by self-renewal capacity and the ability of multilineage differentiation, have gained increased attention in regenerative medicine recently. Histone acetylation modulator proteins (HAMPs) are a protein family that mediates the modification and identification of histone acetylation and participates in various critical cellular processes. Here, we comprehensively surveyed the expression profile of HAMPs during osteoblast differentiation of hDPSCs and found that the HDAC class III pathway was upregulated, whereas the signal transducer and activator of transcription 3 (STAT3) signaling was downregulated during osteogenesis. Further laboratory research demonstrated that Sirtuin-1 (SIRT1), a class III HDAC, was upregulated and STAT3 activation was downregulated during osteogenic differentiation. SIRT1 counteracted the activation of STAT3 to promote osteogenic differentiation of hDPSCs at 7 and 21 days in both Western blot assay and chemical staining, which highlights the promising utility of SIRT1 activators in hDPSCs-based therapies for bone augmentation strategies and provides clinical insights that may lead to the development of osteogenic agents.Dan ZhaoWen KangYiwen WangJiuyu GeJianfeng HuangJie YangWeidong YangXuna TangSijing XieMDPI AGarticledental pulp stem cellsSIRT1STAT3histone acetylation modulator proteinsosteogenic differentiationEngineering (General). Civil engineering (General)TA1-2040ENCoatings, Vol 11, Iss 1353, p 1353 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dental pulp stem cells SIRT1 STAT3 histone acetylation modulator proteins osteogenic differentiation Engineering (General). Civil engineering (General) TA1-2040
spellingShingle	dental pulp stem cells SIRT1 STAT3 histone acetylation modulator proteins osteogenic differentiation Engineering (General). Civil engineering (General) TA1-2040 Dan Zhao Wen Kang Yiwen Wang Jiuyu Ge Jianfeng Huang Jie Yang Weidong Yang Xuna Tang Sijing Xie SIRT1 Promotes Osteogenic Differentiation in Human Dental Pulp Stem Cells through Counteracting the Activation of STAT3
description	Human dental pulp stem cells (hDPSCs), which are characterized by self-renewal capacity and the ability of multilineage differentiation, have gained increased attention in regenerative medicine recently. Histone acetylation modulator proteins (HAMPs) are a protein family that mediates the modification and identification of histone acetylation and participates in various critical cellular processes. Here, we comprehensively surveyed the expression profile of HAMPs during osteoblast differentiation of hDPSCs and found that the HDAC class III pathway was upregulated, whereas the signal transducer and activator of transcription 3 (STAT3) signaling was downregulated during osteogenesis. Further laboratory research demonstrated that Sirtuin-1 (SIRT1), a class III HDAC, was upregulated and STAT3 activation was downregulated during osteogenic differentiation. SIRT1 counteracted the activation of STAT3 to promote osteogenic differentiation of hDPSCs at 7 and 21 days in both Western blot assay and chemical staining, which highlights the promising utility of SIRT1 activators in hDPSCs-based therapies for bone augmentation strategies and provides clinical insights that may lead to the development of osteogenic agents.
format	article
author	Dan Zhao Wen Kang Yiwen Wang Jiuyu Ge Jianfeng Huang Jie Yang Weidong Yang Xuna Tang Sijing Xie
author_facet	Dan Zhao Wen Kang Yiwen Wang Jiuyu Ge Jianfeng Huang Jie Yang Weidong Yang Xuna Tang Sijing Xie
author_sort	Dan Zhao
title	SIRT1 Promotes Osteogenic Differentiation in Human Dental Pulp Stem Cells through Counteracting the Activation of STAT3
title_short	SIRT1 Promotes Osteogenic Differentiation in Human Dental Pulp Stem Cells through Counteracting the Activation of STAT3
title_full	SIRT1 Promotes Osteogenic Differentiation in Human Dental Pulp Stem Cells through Counteracting the Activation of STAT3
title_fullStr	SIRT1 Promotes Osteogenic Differentiation in Human Dental Pulp Stem Cells through Counteracting the Activation of STAT3
title_full_unstemmed	SIRT1 Promotes Osteogenic Differentiation in Human Dental Pulp Stem Cells through Counteracting the Activation of STAT3
title_sort	sirt1 promotes osteogenic differentiation in human dental pulp stem cells through counteracting the activation of stat3
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/de40f411780a4a4780893765f509c150
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SIRT1 Promotes Osteogenic Differentiation in Human Dental Pulp Stem Cells through Counteracting the Activation of STAT3

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