Spatiotemporal sensitivity of mesoderm specification to FGFR signalling in the Drosophila embryo

Abstract Development of the Drosophila embryonic mesoderm is controlled through both internal and external inputs to the mesoderm. One such factor is Heartless (Htl), a Fibroblast Growth Factor Receptor (FGFR) expressed in the mesoderm. Although Htl has been extensively studied, the dynamics of its...

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Autores principales:	V. Yadav, N. Tolwinski, T. E. Saunders
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/de43e1b8393048f295eeee8d523ea031
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spelling	oai:doaj.org-article:de43e1b8393048f295eeee8d523ea0312021-12-02T15:22:56ZSpatiotemporal sensitivity of mesoderm specification to FGFR signalling in the Drosophila embryo10.1038/s41598-021-93512-12045-2322https://doaj.org/article/de43e1b8393048f295eeee8d523ea0312021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93512-1https://doaj.org/toc/2045-2322Abstract Development of the Drosophila embryonic mesoderm is controlled through both internal and external inputs to the mesoderm. One such factor is Heartless (Htl), a Fibroblast Growth Factor Receptor (FGFR) expressed in the mesoderm. Although Htl has been extensively studied, the dynamics of its action are poorly understood after the initial phases of mesoderm formation and spreading. To begin to address this challenge, we have developed an optogenetic version of the FGFR Heartless in Drosophila (Opto-htl). Opto-htl enables us to activate the FGFR pathway in selective spatial (~ 35 μm section from one of the lateral sides of the embryo) and temporal domains (ranging from 40 min to 14 h) during embryogenesis. Importantly, the effects can be tuned by the intensity of light-activation, making this approach significantly more flexible than other genetic approaches. We performed controlled perturbations to the FGFR pathway to define the contribution of Htl signalling to the formation of the developing embryonic heart and somatic muscles. We find a direct correlation between Htl signalling dosage and number of Tinman-positive heart cells specified. Opto-htl activation favours the specification of Tinman positive cardioblasts and eliminates Eve-positive DA1 muscles. This effect is seen to increase progressively with increasing light intensity. Therefore, fine tuning of phenotypic responses to varied Htl signalling dosage can be achieved more conveniently than with other genetic approaches. Overall, Opto-htl is a powerful new tool for dissecting the role of FGFR signalling during development.V. YadavN. TolwinskiT. E. SaundersNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q V. Yadav N. Tolwinski T. E. Saunders Spatiotemporal sensitivity of mesoderm specification to FGFR signalling in the Drosophila embryo
description	Abstract Development of the Drosophila embryonic mesoderm is controlled through both internal and external inputs to the mesoderm. One such factor is Heartless (Htl), a Fibroblast Growth Factor Receptor (FGFR) expressed in the mesoderm. Although Htl has been extensively studied, the dynamics of its action are poorly understood after the initial phases of mesoderm formation and spreading. To begin to address this challenge, we have developed an optogenetic version of the FGFR Heartless in Drosophila (Opto-htl). Opto-htl enables us to activate the FGFR pathway in selective spatial (~ 35 μm section from one of the lateral sides of the embryo) and temporal domains (ranging from 40 min to 14 h) during embryogenesis. Importantly, the effects can be tuned by the intensity of light-activation, making this approach significantly more flexible than other genetic approaches. We performed controlled perturbations to the FGFR pathway to define the contribution of Htl signalling to the formation of the developing embryonic heart and somatic muscles. We find a direct correlation between Htl signalling dosage and number of Tinman-positive heart cells specified. Opto-htl activation favours the specification of Tinman positive cardioblasts and eliminates Eve-positive DA1 muscles. This effect is seen to increase progressively with increasing light intensity. Therefore, fine tuning of phenotypic responses to varied Htl signalling dosage can be achieved more conveniently than with other genetic approaches. Overall, Opto-htl is a powerful new tool for dissecting the role of FGFR signalling during development.
format	article
author	V. Yadav N. Tolwinski T. E. Saunders
author_facet	V. Yadav N. Tolwinski T. E. Saunders
author_sort	V. Yadav
title	Spatiotemporal sensitivity of mesoderm specification to FGFR signalling in the Drosophila embryo
title_short	Spatiotemporal sensitivity of mesoderm specification to FGFR signalling in the Drosophila embryo
title_full	Spatiotemporal sensitivity of mesoderm specification to FGFR signalling in the Drosophila embryo
title_fullStr	Spatiotemporal sensitivity of mesoderm specification to FGFR signalling in the Drosophila embryo
title_full_unstemmed	Spatiotemporal sensitivity of mesoderm specification to FGFR signalling in the Drosophila embryo
title_sort	spatiotemporal sensitivity of mesoderm specification to fgfr signalling in the drosophila embryo
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/de43e1b8393048f295eeee8d523ea031
work_keys_str_mv	AT vyadav spatiotemporalsensitivityofmesodermspecificationtofgfrsignallinginthedrosophilaembryo AT ntolwinski spatiotemporalsensitivityofmesodermspecificationtofgfrsignallinginthedrosophilaembryo AT tesaunders spatiotemporalsensitivityofmesodermspecificationtofgfrsignallinginthedrosophilaembryo
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Spatiotemporal sensitivity of mesoderm specification to FGFR signalling in the Drosophila embryo

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