Impact of refractive index increment on the determination of molecular weight of hyaluronic acid by muti-angle laser light-scattering technique

Impact of refractive index increment on the determination of molecular weight of hyaluronic acid by muti-angle laser light-scattering technique

Abstract Hyaluronic acid (HA) is applied in a number of medical applications and HA of different molecular weight (Mw) are used in different pharmaceutical preparations. In determination of Mw by muti-angle laser light-scattering (MALS), refractive index increment (dn/dc) is an important parameter f...

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Main Authors: Ying Han, Dejie Li, Deqiang Li, Wenwen Chen, Shu’e Mu, Yuqin Chen, Jinling Chai
Format: article
Language:EN
Published: Nature Portfolio 2020
Subjects:
Medicine
R
Science
Q
Online Access:https://doaj.org/article/de449e3d55a14eb2bea63ec0877ac7fc
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spelling oai:doaj.org-article:de449e3d55a14eb2bea63ec0877ac7fc2021-12-02T14:06:58ZImpact of refractive index increment on the determination of molecular weight of hyaluronic acid by muti-angle laser light-scattering technique10.1038/s41598-020-58992-72045-2322https://doaj.org/article/de449e3d55a14eb2bea63ec0877ac7fc2020-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-58992-7https://doaj.org/toc/2045-2322Abstract Hyaluronic acid (HA) is applied in a number of medical applications and HA of different molecular weight (Mw) are used in different pharmaceutical preparations. In determination of Mw by muti-angle laser light-scattering (MALS), refractive index increment (dn/dc) is an important parameter for accuracy. Herein, the influence of dn/dc on the Mw of HA in stroke-physiological saline solution is investigated by MALS in this work. Additionally, the Mw variation of HA in the manufacturing process of preparations is measured. It is shown that each HA sample corresponds to a specific value of dn/dc, which is varied from 1.38 to 1.74 L/g with the Mw increasing from 13.5 to 2840 kDa in solution. It is indicated by the results from both MALS approach and viscometry that appropriate dn/dc should be selected for Mw determination. In steam sterilization process of preparations at 121 °C, the Mw and conformation of HA can be accurately and rapidly determined by MALS. This work provides a precise method to determine the Mw of HA in the medical applications and preparation industries.Ying HanDejie LiDeqiang LiWenwen ChenShu’e MuYuqin ChenJinling ChaiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-8 (2020)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Ying Han
Dejie Li
Deqiang Li
Wenwen Chen
Shu’e Mu
Yuqin Chen
Jinling Chai
Impact of refractive index increment on the determination of molecular weight of hyaluronic acid by muti-angle laser light-scattering technique
description Abstract Hyaluronic acid (HA) is applied in a number of medical applications and HA of different molecular weight (Mw) are used in different pharmaceutical preparations. In determination of Mw by muti-angle laser light-scattering (MALS), refractive index increment (dn/dc) is an important parameter for accuracy. Herein, the influence of dn/dc on the Mw of HA in stroke-physiological saline solution is investigated by MALS in this work. Additionally, the Mw variation of HA in the manufacturing process of preparations is measured. It is shown that each HA sample corresponds to a specific value of dn/dc, which is varied from 1.38 to 1.74 L/g with the Mw increasing from 13.5 to 2840 kDa in solution. It is indicated by the results from both MALS approach and viscometry that appropriate dn/dc should be selected for Mw determination. In steam sterilization process of preparations at 121 °C, the Mw and conformation of HA can be accurately and rapidly determined by MALS. This work provides a precise method to determine the Mw of HA in the medical applications and preparation industries.
format article
author Ying Han
Dejie Li
Deqiang Li
Wenwen Chen
Shu’e Mu
Yuqin Chen
Jinling Chai
author_facet Ying Han
Dejie Li
Deqiang Li
Wenwen Chen
Shu’e Mu
Yuqin Chen
Jinling Chai
author_sort Ying Han
title Impact of refractive index increment on the determination of molecular weight of hyaluronic acid by muti-angle laser light-scattering technique
title_short Impact of refractive index increment on the determination of molecular weight of hyaluronic acid by muti-angle laser light-scattering technique
title_full Impact of refractive index increment on the determination of molecular weight of hyaluronic acid by muti-angle laser light-scattering technique
title_fullStr Impact of refractive index increment on the determination of molecular weight of hyaluronic acid by muti-angle laser light-scattering technique
title_full_unstemmed Impact of refractive index increment on the determination of molecular weight of hyaluronic acid by muti-angle laser light-scattering technique
title_sort impact of refractive index increment on the determination of molecular weight of hyaluronic acid by muti-angle laser light-scattering technique
publisher Nature Portfolio
publishDate 2020
url https://doaj.org/article/de449e3d55a14eb2bea63ec0877ac7fc
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AT dejieli impactofrefractiveindexincrementonthedeterminationofmolecularweightofhyaluronicacidbymutianglelaserlightscatteringtechnique
AT deqiangli impactofrefractiveindexincrementonthedeterminationofmolecularweightofhyaluronicacidbymutianglelaserlightscatteringtechnique
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