Laboratory identification and clinical characteristics of Streptococcus bovis/Streptococcus equinus complex bacteremia: a retrospective, multicenter study in Hiroshima, Japan

Abstract Background Bacteremia due to the Streptococcus bovis/Streptococcus equinus complex (SBSEC) is associated with specific diseases, such as colorectal cancer and infective endocarditis. This study aimed to evaluate the clinical characteristics of SBSEC bacteremia and the accuracy of identifica...

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Autores principales:	Yuki Kaiki, Hiroki Kitagawa, Kayoko Tadera, Hiroyuki Taogoshi, Mitsuyasu Ikeda, Mikihiro Kano, Toshie Harino, Toshihito Nomura, Keitaro Omori, Norifumi Shigemoto, Shinya Takahashi, Hiroki Ohge
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Streptococcus bovis Bloodstream infection MALDI-TOF MS sodA Colorectal cancer Infective endocarditis Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/de44d9abf5c7476885c4ca4ee447c393
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spelling	oai:doaj.org-article:de44d9abf5c7476885c4ca4ee447c3932021-11-28T12:41:44ZLaboratory identification and clinical characteristics of Streptococcus bovis/Streptococcus equinus complex bacteremia: a retrospective, multicenter study in Hiroshima, Japan10.1186/s12879-021-06880-41471-2334https://doaj.org/article/de44d9abf5c7476885c4ca4ee447c3932021-11-01T00:00:00Zhttps://doi.org/10.1186/s12879-021-06880-4https://doaj.org/toc/1471-2334Abstract Background Bacteremia due to the Streptococcus bovis/Streptococcus equinus complex (SBSEC) is associated with specific diseases, such as colorectal cancer and infective endocarditis. This study aimed to evaluate the clinical characteristics of SBSEC bacteremia and the accuracy of identification of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and phenotypic identification systems for SBSEC isolates. Methods We analyzed patients with SBSEC bacteremia retrospectively between 2012 and 2019 at three hospitals in Japan. We re-identified each SBSEC isolate using sequencing superoxide dismutase (sodA) analysis, MALDI-TOF MS using the MALDI Biotyper, and phenotypic identification using the VITEK2. Results During the study period, 39 patients with SBSEC bacteremia were identified. S. gallolyticus subsp. pasteurianus (SGSP, n = 29), S. gallolyticus subsp. gallolyticus (SGSG, n = 5), S. lutetiensis (SL, n = 4), and S. infantarius subsp. infantarius (n = 1) were identified using sodA sequencing analysis. Primary bacteremia (36%) was the most common cause of bacteremia, followed by infective endocarditis (26%) and biliary tract infections (23%). Colorectal cancer was associated significantly with SGSG bacteremia, while the sources of bacteremia were similar in each SBSEC subspecies. The MALDI Biotyper was significantly more accurate in identifying the SBSEC isolates at the subspecies level compared to the VITEK2 (92% vs. 67%, P = 0.010). In contrast, there were no significant differences in the rates of correct identification of the SBSEC isolates at the species level between the MALDI Biotyper and the VITEK2 (100% vs. 87%, P = 0.055). Conclusions Bacteremia with SGSG was associated with colorectal cancer, and the sources of bacteremia were similar in each SBSEC subspecies. The MALDI-TOF MS was significantly more accurate in identifying SBSEC isolates at the subspecies level than the phenotypic identification systems. The accurate identification of SBSEC isolates using the MALDI-TOF MS and phenotypic identification systems was sufficient at the species level, but it was insufficient at the subspecies level. Therefore, it may be reasonable for clinicians to perform echocardiographies and colonoscopies in all patients with SBSEC bacteremia.Yuki KaikiHiroki KitagawaKayoko TaderaHiroyuki TaogoshiMitsuyasu IkedaMikihiro KanoToshie HarinoToshihito NomuraKeitaro OmoriNorifumi ShigemotoShinya TakahashiHiroki OhgeBMCarticleStreptococcus bovisBloodstream infectionMALDI-TOF MSsodAColorectal cancerInfective endocarditisInfectious and parasitic diseasesRC109-216ENBMC Infectious Diseases, Vol 21, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Streptococcus bovis Bloodstream infection MALDI-TOF MS sodA Colorectal cancer Infective endocarditis Infectious and parasitic diseases RC109-216
spellingShingle	Streptococcus bovis Bloodstream infection MALDI-TOF MS sodA Colorectal cancer Infective endocarditis Infectious and parasitic diseases RC109-216 Yuki Kaiki Hiroki Kitagawa Kayoko Tadera Hiroyuki Taogoshi Mitsuyasu Ikeda Mikihiro Kano Toshie Harino Toshihito Nomura Keitaro Omori Norifumi Shigemoto Shinya Takahashi Hiroki Ohge Laboratory identification and clinical characteristics of Streptococcus bovis/Streptococcus equinus complex bacteremia: a retrospective, multicenter study in Hiroshima, Japan
description	Abstract Background Bacteremia due to the Streptococcus bovis/Streptococcus equinus complex (SBSEC) is associated with specific diseases, such as colorectal cancer and infective endocarditis. This study aimed to evaluate the clinical characteristics of SBSEC bacteremia and the accuracy of identification of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and phenotypic identification systems for SBSEC isolates. Methods We analyzed patients with SBSEC bacteremia retrospectively between 2012 and 2019 at three hospitals in Japan. We re-identified each SBSEC isolate using sequencing superoxide dismutase (sodA) analysis, MALDI-TOF MS using the MALDI Biotyper, and phenotypic identification using the VITEK2. Results During the study period, 39 patients with SBSEC bacteremia were identified. S. gallolyticus subsp. pasteurianus (SGSP, n = 29), S. gallolyticus subsp. gallolyticus (SGSG, n = 5), S. lutetiensis (SL, n = 4), and S. infantarius subsp. infantarius (n = 1) were identified using sodA sequencing analysis. Primary bacteremia (36%) was the most common cause of bacteremia, followed by infective endocarditis (26%) and biliary tract infections (23%). Colorectal cancer was associated significantly with SGSG bacteremia, while the sources of bacteremia were similar in each SBSEC subspecies. The MALDI Biotyper was significantly more accurate in identifying the SBSEC isolates at the subspecies level compared to the VITEK2 (92% vs. 67%, P = 0.010). In contrast, there were no significant differences in the rates of correct identification of the SBSEC isolates at the species level between the MALDI Biotyper and the VITEK2 (100% vs. 87%, P = 0.055). Conclusions Bacteremia with SGSG was associated with colorectal cancer, and the sources of bacteremia were similar in each SBSEC subspecies. The MALDI-TOF MS was significantly more accurate in identifying SBSEC isolates at the subspecies level than the phenotypic identification systems. The accurate identification of SBSEC isolates using the MALDI-TOF MS and phenotypic identification systems was sufficient at the species level, but it was insufficient at the subspecies level. Therefore, it may be reasonable for clinicians to perform echocardiographies and colonoscopies in all patients with SBSEC bacteremia.
format	article
author	Yuki Kaiki Hiroki Kitagawa Kayoko Tadera Hiroyuki Taogoshi Mitsuyasu Ikeda Mikihiro Kano Toshie Harino Toshihito Nomura Keitaro Omori Norifumi Shigemoto Shinya Takahashi Hiroki Ohge
author_facet	Yuki Kaiki Hiroki Kitagawa Kayoko Tadera Hiroyuki Taogoshi Mitsuyasu Ikeda Mikihiro Kano Toshie Harino Toshihito Nomura Keitaro Omori Norifumi Shigemoto Shinya Takahashi Hiroki Ohge
author_sort	Yuki Kaiki
title	Laboratory identification and clinical characteristics of Streptococcus bovis/Streptococcus equinus complex bacteremia: a retrospective, multicenter study in Hiroshima, Japan
title_short	Laboratory identification and clinical characteristics of Streptococcus bovis/Streptococcus equinus complex bacteremia: a retrospective, multicenter study in Hiroshima, Japan
title_full	Laboratory identification and clinical characteristics of Streptococcus bovis/Streptococcus equinus complex bacteremia: a retrospective, multicenter study in Hiroshima, Japan
title_fullStr	Laboratory identification and clinical characteristics of Streptococcus bovis/Streptococcus equinus complex bacteremia: a retrospective, multicenter study in Hiroshima, Japan
title_full_unstemmed	Laboratory identification and clinical characteristics of Streptococcus bovis/Streptococcus equinus complex bacteremia: a retrospective, multicenter study in Hiroshima, Japan
title_sort	laboratory identification and clinical characteristics of streptococcus bovis/streptococcus equinus complex bacteremia: a retrospective, multicenter study in hiroshima, japan
publisher	BMC
publishDate	2021
url	https://doaj.org/article/de44d9abf5c7476885c4ca4ee447c393
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Laboratory identification and clinical characteristics of Streptococcus bovis/Streptococcus equinus complex bacteremia: a retrospective, multicenter study in Hiroshima, Japan

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