Curcumin protects rat hippocampal neurons against pseudorabies virus by regulating the BDNF/TrkB pathway

Abstract Pseudorabies virus (PRV) infection can elicit nervous system disorders. Curcumin has been reported to have neuroprotective effects. However, whether curcumin can protect neurons against PRV infection and the underlying mechanisms remain unclear. In the present study, for the first time, the...

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Autores principales:	Bingjie Yang, Guodong Luo, Chen Zhang, Luqiu Feng, Xianmei Luo, Ling Gan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/de4b5c545a6e411cbdbc2a9e4d4f6e16
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spelling	oai:doaj.org-article:de4b5c545a6e411cbdbc2a9e4d4f6e162021-12-02T12:42:19ZCurcumin protects rat hippocampal neurons against pseudorabies virus by regulating the BDNF/TrkB pathway10.1038/s41598-020-78903-02045-2322https://doaj.org/article/de4b5c545a6e411cbdbc2a9e4d4f6e162020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78903-0https://doaj.org/toc/2045-2322Abstract Pseudorabies virus (PRV) infection can elicit nervous system disorders. Curcumin has been reported to have neuroprotective effects. However, whether curcumin can protect neurons against PRV infection and the underlying mechanisms remain unclear. In the present study, for the first time, the protective effects of curcumin against PRV-induced oxidative stress, apoptosis, and mitochondrial dysfunction in rat hippocampal neurons and the brain-derived neurotrophic factor (BDNF)/tropomyosin-related kinase B (TrkB) pathway were investigated. Results indicated that PRV with a titer of 3.06 × 106 TCID50 (50% tissue culture infective dose) induced oxidative damage of hippocampal neurons 2 h post-infection and that 10 μM curcumin improved the viability of PRV-infected hippocampal neurons. Blocking the BDNF/TrkB pathway reversed the neuroprotective effects of curcumin, which were imparted by decreasing the PRV-induced upregulation of nitric oxide synthase expression, repressing the PRV-activated mitochondrial apoptotic pathway, and mitochondrial dysfunction. To conclude, curcumin exhibited a neuroprotective role against PRV infection by upregulating the BDNF/TrkB pathway. This study provides insight into the anti-PRV neuroprotective application of curcumin and the underlying mechanism in the prophylaxis and treatment of neurological disorders caused by PRV infection.Bingjie YangGuodong LuoChen ZhangLuqiu FengXianmei LuoLing GanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bingjie Yang Guodong Luo Chen Zhang Luqiu Feng Xianmei Luo Ling Gan Curcumin protects rat hippocampal neurons against pseudorabies virus by regulating the BDNF/TrkB pathway
description	Abstract Pseudorabies virus (PRV) infection can elicit nervous system disorders. Curcumin has been reported to have neuroprotective effects. However, whether curcumin can protect neurons against PRV infection and the underlying mechanisms remain unclear. In the present study, for the first time, the protective effects of curcumin against PRV-induced oxidative stress, apoptosis, and mitochondrial dysfunction in rat hippocampal neurons and the brain-derived neurotrophic factor (BDNF)/tropomyosin-related kinase B (TrkB) pathway were investigated. Results indicated that PRV with a titer of 3.06 × 106 TCID50 (50% tissue culture infective dose) induced oxidative damage of hippocampal neurons 2 h post-infection and that 10 μM curcumin improved the viability of PRV-infected hippocampal neurons. Blocking the BDNF/TrkB pathway reversed the neuroprotective effects of curcumin, which were imparted by decreasing the PRV-induced upregulation of nitric oxide synthase expression, repressing the PRV-activated mitochondrial apoptotic pathway, and mitochondrial dysfunction. To conclude, curcumin exhibited a neuroprotective role against PRV infection by upregulating the BDNF/TrkB pathway. This study provides insight into the anti-PRV neuroprotective application of curcumin and the underlying mechanism in the prophylaxis and treatment of neurological disorders caused by PRV infection.
format	article
author	Bingjie Yang Guodong Luo Chen Zhang Luqiu Feng Xianmei Luo Ling Gan
author_facet	Bingjie Yang Guodong Luo Chen Zhang Luqiu Feng Xianmei Luo Ling Gan
author_sort	Bingjie Yang
title	Curcumin protects rat hippocampal neurons against pseudorabies virus by regulating the BDNF/TrkB pathway
title_short	Curcumin protects rat hippocampal neurons against pseudorabies virus by regulating the BDNF/TrkB pathway
title_full	Curcumin protects rat hippocampal neurons against pseudorabies virus by regulating the BDNF/TrkB pathway
title_fullStr	Curcumin protects rat hippocampal neurons against pseudorabies virus by regulating the BDNF/TrkB pathway
title_full_unstemmed	Curcumin protects rat hippocampal neurons against pseudorabies virus by regulating the BDNF/TrkB pathway
title_sort	curcumin protects rat hippocampal neurons against pseudorabies virus by regulating the bdnf/trkb pathway
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/de4b5c545a6e411cbdbc2a9e4d4f6e16
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Curcumin protects rat hippocampal neurons against pseudorabies virus by regulating the BDNF/TrkB pathway

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