Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection

CRISPR-Cas12a based detection systems can be sensitive down to the picomolar range. Here the authors modify the 3′- and 5′-ends of the crRNA and show this enhances trans-cleavage for improved sensitivity.

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Autores principales:	Long T. Nguyen, Brianna M. Smith, Piyush K. Jain
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/de5c4909e62841dd937390825877c68b
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spelling	oai:doaj.org-article:de5c4909e62841dd937390825877c68b2021-12-02T17:37:14ZEnhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection10.1038/s41467-020-18615-12041-1723https://doaj.org/article/de5c4909e62841dd937390825877c68b2020-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-18615-1https://doaj.org/toc/2041-1723CRISPR-Cas12a based detection systems can be sensitive down to the picomolar range. Here the authors modify the 3′- and 5′-ends of the crRNA and show this enhances trans-cleavage for improved sensitivity.Long T. NguyenBrianna M. SmithPiyush K. JainNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Long T. Nguyen Brianna M. Smith Piyush K. Jain Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection
description	CRISPR-Cas12a based detection systems can be sensitive down to the picomolar range. Here the authors modify the 3′- and 5′-ends of the crRNA and show this enhances trans-cleavage for improved sensitivity.
format	article
author	Long T. Nguyen Brianna M. Smith Piyush K. Jain
author_facet	Long T. Nguyen Brianna M. Smith Piyush K. Jain
author_sort	Long T. Nguyen
title	Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection
title_short	Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection
title_full	Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection
title_fullStr	Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection
title_full_unstemmed	Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection
title_sort	enhancement of trans-cleavage activity of cas12a with engineered crrna enables amplified nucleic acid detection
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/de5c4909e62841dd937390825877c68b
work_keys_str_mv	AT longtnguyen enhancementoftranscleavageactivityofcas12awithengineeredcrrnaenablesamplifiednucleicaciddetection AT briannamsmith enhancementoftranscleavageactivityofcas12awithengineeredcrrnaenablesamplifiednucleicaciddetection AT piyushkjain enhancementoftranscleavageactivityofcas12awithengineeredcrrnaenablesamplifiednucleicaciddetection
_version_	1718379913115860992

Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection

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