Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection
CRISPR-Cas12a based detection systems can be sensitive down to the picomolar range. Here the authors modify the 3′- and 5′-ends of the crRNA and show this enhances trans-cleavage for improved sensitivity.
Guardado en:
Autores principales: | Long T. Nguyen, Brianna M. Smith, Piyush K. Jain |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2020
|
Materias: | |
Acceso en línea: | https://doaj.org/article/de5c4909e62841dd937390825877c68b |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
Ejemplares similares
-
Author Correction: Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection
por: Long T. Nguyen, et al.
Publicado: (2020) -
Definition of CRISPR Cas12a Trans-Cleavage Units to Facilitate CRISPR Diagnostics
por: Hailong Lv, et al.
Publicado: (2021) -
Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis
por: Chunyu Liao, et al.
Publicado: (2019) -
Extension of the crRNA enhances Cpf1 gene editing in vitro and in vivo
por: Hyo Min Park, et al.
Publicado: (2018) -
Programmable sequential mutagenesis by inducible Cpf1 crRNA array inversion
por: Ryan D. Chow, et al.
Publicado: (2018)