Complete genome sequence analysis of Nocardia brasiliensis HUJEG-1 reveals a saprobic lifestyle and the genes needed for human pathogenesis.

Nocardia brasiliensis is an important etiologic agent of mycetoma. These bacteria live as a saprobe in soil or organic material and enter the tissue via minor trauma. Mycetoma is characterized by tumefaction and the production of fistula and abscesses, with no spontaneous cure. By using mass sequenc...

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Autores principales:	Lucio Vera-Cabrera, Rocio Ortiz-Lopez, Ramiro Elizondo-Gonzalez, Jorge Ocampo-Candiani
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/de62808511df49ec8047746c179a81bf
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spelling	oai:doaj.org-article:de62808511df49ec8047746c179a81bf2021-11-18T07:43:12ZComplete genome sequence analysis of Nocardia brasiliensis HUJEG-1 reveals a saprobic lifestyle and the genes needed for human pathogenesis.1932-620310.1371/journal.pone.0065425https://doaj.org/article/de62808511df49ec8047746c179a81bf2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23755230/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Nocardia brasiliensis is an important etiologic agent of mycetoma. These bacteria live as a saprobe in soil or organic material and enter the tissue via minor trauma. Mycetoma is characterized by tumefaction and the production of fistula and abscesses, with no spontaneous cure. By using mass sequencing, we determined the complete genomic nucleotide sequence of the bacteria. According to our data, the genome is a circular chromosome 9,436,348-bp long with 68% G+C content that encodes 8,414 proteins. We observed orthologs for virulence factors, a higher number of genes involved in lipid biosynthesis and catabolism, and gene clusters for the synthesis of bioactive compounds, such as antibiotics, terpenes, and polyketides. An in silico analysis of the sequence supports the conclusion that the bacteria acquired diverse genes by horizontal transfer from other soil bacteria, even from eukaryotic organisms. The genome composition reflects the evolution of bacteria via the acquisition of a large amount of DNA, which allows it to survive in new ecological niches, including humans.Lucio Vera-CabreraRocio Ortiz-LopezRamiro Elizondo-GonzalezJorge Ocampo-CandianiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e65425 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lucio Vera-Cabrera Rocio Ortiz-Lopez Ramiro Elizondo-Gonzalez Jorge Ocampo-Candiani Complete genome sequence analysis of Nocardia brasiliensis HUJEG-1 reveals a saprobic lifestyle and the genes needed for human pathogenesis.
description	Nocardia brasiliensis is an important etiologic agent of mycetoma. These bacteria live as a saprobe in soil or organic material and enter the tissue via minor trauma. Mycetoma is characterized by tumefaction and the production of fistula and abscesses, with no spontaneous cure. By using mass sequencing, we determined the complete genomic nucleotide sequence of the bacteria. According to our data, the genome is a circular chromosome 9,436,348-bp long with 68% G+C content that encodes 8,414 proteins. We observed orthologs for virulence factors, a higher number of genes involved in lipid biosynthesis and catabolism, and gene clusters for the synthesis of bioactive compounds, such as antibiotics, terpenes, and polyketides. An in silico analysis of the sequence supports the conclusion that the bacteria acquired diverse genes by horizontal transfer from other soil bacteria, even from eukaryotic organisms. The genome composition reflects the evolution of bacteria via the acquisition of a large amount of DNA, which allows it to survive in new ecological niches, including humans.
format	article
author	Lucio Vera-Cabrera Rocio Ortiz-Lopez Ramiro Elizondo-Gonzalez Jorge Ocampo-Candiani
author_facet	Lucio Vera-Cabrera Rocio Ortiz-Lopez Ramiro Elizondo-Gonzalez Jorge Ocampo-Candiani
author_sort	Lucio Vera-Cabrera
title	Complete genome sequence analysis of Nocardia brasiliensis HUJEG-1 reveals a saprobic lifestyle and the genes needed for human pathogenesis.
title_short	Complete genome sequence analysis of Nocardia brasiliensis HUJEG-1 reveals a saprobic lifestyle and the genes needed for human pathogenesis.
title_full	Complete genome sequence analysis of Nocardia brasiliensis HUJEG-1 reveals a saprobic lifestyle and the genes needed for human pathogenesis.
title_fullStr	Complete genome sequence analysis of Nocardia brasiliensis HUJEG-1 reveals a saprobic lifestyle and the genes needed for human pathogenesis.
title_full_unstemmed	Complete genome sequence analysis of Nocardia brasiliensis HUJEG-1 reveals a saprobic lifestyle and the genes needed for human pathogenesis.
title_sort	complete genome sequence analysis of nocardia brasiliensis hujeg-1 reveals a saprobic lifestyle and the genes needed for human pathogenesis.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/de62808511df49ec8047746c179a81bf
work_keys_str_mv	AT lucioveracabrera completegenomesequenceanalysisofnocardiabrasiliensishujeg1revealsasaprobiclifestyleandthegenesneededforhumanpathogenesis AT rocioortizlopez completegenomesequenceanalysisofnocardiabrasiliensishujeg1revealsasaprobiclifestyleandthegenesneededforhumanpathogenesis AT ramiroelizondogonzalez completegenomesequenceanalysisofnocardiabrasiliensishujeg1revealsasaprobiclifestyleandthegenesneededforhumanpathogenesis AT jorgeocampocandiani completegenomesequenceanalysisofnocardiabrasiliensishujeg1revealsasaprobiclifestyleandthegenesneededforhumanpathogenesis
_version_	1718423068520480768

Complete genome sequence analysis of Nocardia brasiliensis HUJEG-1 reveals a saprobic lifestyle and the genes needed for human pathogenesis.

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