An approach to identify SNPs in the gene encoding acetyl-CoA acetyltransferase-2 (ACAT-2) and their proposed role in metabolic processes in pig.

The novel liver protein acetyl-CoA acetyltransferase-2 (ACAT2) is involved in the beta-oxidation and lipid metabolism. Its comprehensive relative expression, in silico non-synonymous single nucleotide polymorphism (nsSNP) analysis, as well as its annotation in terms of metabolic process with another...

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Autores principales:	Simrinder Singh Sodhi, Mrinmoy Ghosh, Ki Duk Song, Neelesh Sharma, Jeong Hyun Kim, Nam Eun Kim, Sung Jin Lee, Chul Woong Kang, Sung Jong Oh, Dong Kee Jeong
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/de63dc40dc9e43d49992536837975ceb
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spelling	oai:doaj.org-article:de63dc40dc9e43d49992536837975ceb2021-11-25T06:07:38ZAn approach to identify SNPs in the gene encoding acetyl-CoA acetyltransferase-2 (ACAT-2) and their proposed role in metabolic processes in pig.1932-620310.1371/journal.pone.0102432https://doaj.org/article/de63dc40dc9e43d49992536837975ceb2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25050817/?tool=EBIhttps://doaj.org/toc/1932-6203The novel liver protein acetyl-CoA acetyltransferase-2 (ACAT2) is involved in the beta-oxidation and lipid metabolism. Its comprehensive relative expression, in silico non-synonymous single nucleotide polymorphism (nsSNP) analysis, as well as its annotation in terms of metabolic process with another protein from the same family, namely, acetyl-CoA acyltransferase-2 (ACAA2) was performed in Sus scrofa. This investigation was conducted to understand the most important nsSNPs of ACAT2 in terms of their effects on metabolic activities and protein conformation. The two most deleterious mutations at residues 122 (I to V) and 281 (R to H) were found in ACAT2. Validation of expression of genes in the laboratory also supported the idea of differential expression of ACAT2 and ACAA2 conceived through the in silico analysis. Analysis of the relative expression of ACAT2 and ACAA2 in the liver tissue of Jeju native pig showed that the former expressed significantly higher (P<0.05). Overall, the computational prediction supported by wet laboratory analysis suggests that ACAT2 might contribute more to metabolic processes than ACAA2 in swine. Further associations of SNPs in ACAT2 with production traits might guide efforts to improve growth performance in Jeju native pigs.Simrinder Singh SodhiMrinmoy GhoshKi Duk SongNeelesh SharmaJeong Hyun KimNam Eun KimSung Jin LeeChul Woong KangSung Jong OhDong Kee JeongPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 7, p e102432 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Simrinder Singh Sodhi Mrinmoy Ghosh Ki Duk Song Neelesh Sharma Jeong Hyun Kim Nam Eun Kim Sung Jin Lee Chul Woong Kang Sung Jong Oh Dong Kee Jeong An approach to identify SNPs in the gene encoding acetyl-CoA acetyltransferase-2 (ACAT-2) and their proposed role in metabolic processes in pig.
description	The novel liver protein acetyl-CoA acetyltransferase-2 (ACAT2) is involved in the beta-oxidation and lipid metabolism. Its comprehensive relative expression, in silico non-synonymous single nucleotide polymorphism (nsSNP) analysis, as well as its annotation in terms of metabolic process with another protein from the same family, namely, acetyl-CoA acyltransferase-2 (ACAA2) was performed in Sus scrofa. This investigation was conducted to understand the most important nsSNPs of ACAT2 in terms of their effects on metabolic activities and protein conformation. The two most deleterious mutations at residues 122 (I to V) and 281 (R to H) were found in ACAT2. Validation of expression of genes in the laboratory also supported the idea of differential expression of ACAT2 and ACAA2 conceived through the in silico analysis. Analysis of the relative expression of ACAT2 and ACAA2 in the liver tissue of Jeju native pig showed that the former expressed significantly higher (P<0.05). Overall, the computational prediction supported by wet laboratory analysis suggests that ACAT2 might contribute more to metabolic processes than ACAA2 in swine. Further associations of SNPs in ACAT2 with production traits might guide efforts to improve growth performance in Jeju native pigs.
format	article
author	Simrinder Singh Sodhi Mrinmoy Ghosh Ki Duk Song Neelesh Sharma Jeong Hyun Kim Nam Eun Kim Sung Jin Lee Chul Woong Kang Sung Jong Oh Dong Kee Jeong
author_facet	Simrinder Singh Sodhi Mrinmoy Ghosh Ki Duk Song Neelesh Sharma Jeong Hyun Kim Nam Eun Kim Sung Jin Lee Chul Woong Kang Sung Jong Oh Dong Kee Jeong
author_sort	Simrinder Singh Sodhi
title	An approach to identify SNPs in the gene encoding acetyl-CoA acetyltransferase-2 (ACAT-2) and their proposed role in metabolic processes in pig.
title_short	An approach to identify SNPs in the gene encoding acetyl-CoA acetyltransferase-2 (ACAT-2) and their proposed role in metabolic processes in pig.
title_full	An approach to identify SNPs in the gene encoding acetyl-CoA acetyltransferase-2 (ACAT-2) and their proposed role in metabolic processes in pig.
title_fullStr	An approach to identify SNPs in the gene encoding acetyl-CoA acetyltransferase-2 (ACAT-2) and their proposed role in metabolic processes in pig.
title_full_unstemmed	An approach to identify SNPs in the gene encoding acetyl-CoA acetyltransferase-2 (ACAT-2) and their proposed role in metabolic processes in pig.
title_sort	approach to identify snps in the gene encoding acetyl-coa acetyltransferase-2 (acat-2) and their proposed role in metabolic processes in pig.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/de63dc40dc9e43d49992536837975ceb
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An approach to identify SNPs in the gene encoding acetyl-CoA acetyltransferase-2 (ACAT-2) and their proposed role in metabolic processes in pig.

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