Ectopic expression of a novel cold-resistance protein 1 from Brassica oleracea promotes tolerance to chilling stress in transgenic tomato

Abstract Cold stress is considered as one of the major environmental factors that adversely affects the plant growth and distribution. Therefore, there arises an immediate need to cultivate effective strategies aimed at developing stress-tolerant crops that would boost the production and minimise th...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Umer Majeed Wani, Sheikh Tahir Majeed, Vaseem Raja, Zubair Ahmad Wani, Nelofer Jan, Khursid Iqbal Andrabi, Riffat John
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/de63f560579749d995cd60c24436755e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:de63f560579749d995cd60c24436755e
record_format	dspace
spelling	oai:doaj.org-article:de63f560579749d995cd60c24436755e2021-12-02T17:08:44ZEctopic expression of a novel cold-resistance protein 1 from Brassica oleracea promotes tolerance to chilling stress in transgenic tomato10.1038/s41598-021-96102-32045-2322https://doaj.org/article/de63f560579749d995cd60c24436755e2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96102-3https://doaj.org/toc/2045-2322Abstract Cold stress is considered as one of the major environmental factors that adversely affects the plant growth and distribution. Therefore, there arises an immediate need to cultivate effective strategies aimed at developing stress-tolerant crops that would boost the production and minimise the risks associated with cold stress. In this study, a novel cold-responsive protein1 (BoCRP1) isolated from Brassica oleracea was ectopically expressed in a cold susceptible tomato genotype Shalimar 1 and its function was investigated in response to chilling stress. BoCRP1 was constitutively expressed in all the tissues of B. oleracea including leaf, root and stem. However, its expression was found to be significantly increased in response to cold stress. Moreover, transgenic tomato plants expressing BoCRP1 exhibited increased tolerance to chilling stress (4 °C) with an overall improved rate of seed germination, increased root length, reduced membrane damage and increased accumulation of osmoprotectants. Furthermore, we observed increased transcript levels of stress responsive genes and enhanced accumulation of reactive oxygen species scavenging enzymes in transgenic plants on exposure to chilling stress. Taken together, these results strongly suggest that BoCRP1 is a promising candidate gene to improve the cold stress tolerance in tomato.Umer Majeed WaniSheikh Tahir MajeedVaseem RajaZubair Ahmad WaniNelofer JanKhursid Iqbal AndrabiRiffat JohnNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Umer Majeed Wani Sheikh Tahir Majeed Vaseem Raja Zubair Ahmad Wani Nelofer Jan Khursid Iqbal Andrabi Riffat John Ectopic expression of a novel cold-resistance protein 1 from Brassica oleracea promotes tolerance to chilling stress in transgenic tomato
description	Abstract Cold stress is considered as one of the major environmental factors that adversely affects the plant growth and distribution. Therefore, there arises an immediate need to cultivate effective strategies aimed at developing stress-tolerant crops that would boost the production and minimise the risks associated with cold stress. In this study, a novel cold-responsive protein1 (BoCRP1) isolated from Brassica oleracea was ectopically expressed in a cold susceptible tomato genotype Shalimar 1 and its function was investigated in response to chilling stress. BoCRP1 was constitutively expressed in all the tissues of B. oleracea including leaf, root and stem. However, its expression was found to be significantly increased in response to cold stress. Moreover, transgenic tomato plants expressing BoCRP1 exhibited increased tolerance to chilling stress (4 °C) with an overall improved rate of seed germination, increased root length, reduced membrane damage and increased accumulation of osmoprotectants. Furthermore, we observed increased transcript levels of stress responsive genes and enhanced accumulation of reactive oxygen species scavenging enzymes in transgenic plants on exposure to chilling stress. Taken together, these results strongly suggest that BoCRP1 is a promising candidate gene to improve the cold stress tolerance in tomato.
format	article
author	Umer Majeed Wani Sheikh Tahir Majeed Vaseem Raja Zubair Ahmad Wani Nelofer Jan Khursid Iqbal Andrabi Riffat John
author_facet	Umer Majeed Wani Sheikh Tahir Majeed Vaseem Raja Zubair Ahmad Wani Nelofer Jan Khursid Iqbal Andrabi Riffat John
author_sort	Umer Majeed Wani
title	Ectopic expression of a novel cold-resistance protein 1 from Brassica oleracea promotes tolerance to chilling stress in transgenic tomato
title_short	Ectopic expression of a novel cold-resistance protein 1 from Brassica oleracea promotes tolerance to chilling stress in transgenic tomato
title_full	Ectopic expression of a novel cold-resistance protein 1 from Brassica oleracea promotes tolerance to chilling stress in transgenic tomato
title_fullStr	Ectopic expression of a novel cold-resistance protein 1 from Brassica oleracea promotes tolerance to chilling stress in transgenic tomato
title_full_unstemmed	Ectopic expression of a novel cold-resistance protein 1 from Brassica oleracea promotes tolerance to chilling stress in transgenic tomato
title_sort	ectopic expression of a novel cold-resistance protein 1 from brassica oleracea promotes tolerance to chilling stress in transgenic tomato
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/de63f560579749d995cd60c24436755e
work_keys_str_mv	AT umermajeedwani ectopicexpressionofanovelcoldresistanceprotein1frombrassicaoleraceapromotestolerancetochillingstressintransgenictomato AT sheikhtahirmajeed ectopicexpressionofanovelcoldresistanceprotein1frombrassicaoleraceapromotestolerancetochillingstressintransgenictomato AT vaseemraja ectopicexpressionofanovelcoldresistanceprotein1frombrassicaoleraceapromotestolerancetochillingstressintransgenictomato AT zubairahmadwani ectopicexpressionofanovelcoldresistanceprotein1frombrassicaoleraceapromotestolerancetochillingstressintransgenictomato AT neloferjan ectopicexpressionofanovelcoldresistanceprotein1frombrassicaoleraceapromotestolerancetochillingstressintransgenictomato AT khursidiqbalandrabi ectopicexpressionofanovelcoldresistanceprotein1frombrassicaoleraceapromotestolerancetochillingstressintransgenictomato AT riffatjohn ectopicexpressionofanovelcoldresistanceprotein1frombrassicaoleraceapromotestolerancetochillingstressintransgenictomato
_version_	1718381528428314624

Ectopic expression of a novel cold-resistance protein 1 from Brassica oleracea promotes tolerance to chilling stress in transgenic tomato

Ejemplares similares