Nanodrug Transmembrane Transport Research Based on Fluorescence Correlation Spectroscopy

Although conventional fluorescence intensity imaging can be used to qualitatively study the drug toxicity of nanodrug carrier systems at the single-cell level, it has limitations for studying nanodrug transport across membranes. Fluorescence correlation spectroscopy (FCS) can provide quantitative in...

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Autores principales:	Xinwei Gao, Yanfeng Liu, Jia Zhang, Luwei Wang, Yong Guo, Yinru Zhu, Zhigang Yang, Wei Yan, Junle Qu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	carbon dots fluorescence correlation spectroscopy doxorubicin transmembrane transport Chemical technology TP1-1185 Chemical engineering TP155-156
Acceso en línea:	https://doaj.org/article/de694840986a4daab2b80181bc9be4d3
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spelling	oai:doaj.org-article:de694840986a4daab2b80181bc9be4d32021-11-25T18:20:07ZNanodrug Transmembrane Transport Research Based on Fluorescence Correlation Spectroscopy10.3390/membranes111108912077-0375https://doaj.org/article/de694840986a4daab2b80181bc9be4d32021-11-01T00:00:00Zhttps://www.mdpi.com/2077-0375/11/11/891https://doaj.org/toc/2077-0375Although conventional fluorescence intensity imaging can be used to qualitatively study the drug toxicity of nanodrug carrier systems at the single-cell level, it has limitations for studying nanodrug transport across membranes. Fluorescence correlation spectroscopy (FCS) can provide quantitative information on nanodrug concentration and diffusion in a small area of the cell membrane; thus, it is an ideal tool for studying drug transport across the membrane. In this paper, the FCS method was used to measure the diffusion coefficients and concentrations of carbon dots (CDs), doxorubicin (DOX) and CDs-DOX composites in living cells (COS7 and U<sub>2</sub>OS) for the first time. The drug concentration and diffusion coefficient in living cells determined by FCS measurements indicated that the CDs-DOX composite distinctively improved the transmembrane efficiency and rate of drug molecules, in accordance with the conclusions drawn from the fluorescence imaging results. Furthermore, the effects of pH values and ATP concentrations on drug transport across the membrane were also studied. Compared with free DOX under acidic conditions, the CDs-DOX complex has higher cellular uptake and better transmembrane efficacy in U<sub>2</sub>OS cells. Additionally, high concentrations of ATP will cause negative changes in cell membrane permeability, which will hinder the transmembrane transport of CDs and DOX and delay the rapid diffusion of CDs-DOX. The results of this study show that the FCS method can be utilized as a powerful tool for studying the expansion and transport of nanodrugs in living cells, and might provide a new drug exploitation strategy for cancer treatment in vivo.Xinwei GaoYanfeng LiuJia ZhangLuwei WangYong GuoYinru ZhuZhigang YangWei YanJunle QuMDPI AGarticlecarbon dotsfluorescence correlation spectroscopydoxorubicintransmembrane transportChemical technologyTP1-1185Chemical engineeringTP155-156ENMembranes, Vol 11, Iss 891, p 891 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	carbon dots fluorescence correlation spectroscopy doxorubicin transmembrane transport Chemical technology TP1-1185 Chemical engineering TP155-156
spellingShingle	carbon dots fluorescence correlation spectroscopy doxorubicin transmembrane transport Chemical technology TP1-1185 Chemical engineering TP155-156 Xinwei Gao Yanfeng Liu Jia Zhang Luwei Wang Yong Guo Yinru Zhu Zhigang Yang Wei Yan Junle Qu Nanodrug Transmembrane Transport Research Based on Fluorescence Correlation Spectroscopy
description	Although conventional fluorescence intensity imaging can be used to qualitatively study the drug toxicity of nanodrug carrier systems at the single-cell level, it has limitations for studying nanodrug transport across membranes. Fluorescence correlation spectroscopy (FCS) can provide quantitative information on nanodrug concentration and diffusion in a small area of the cell membrane; thus, it is an ideal tool for studying drug transport across the membrane. In this paper, the FCS method was used to measure the diffusion coefficients and concentrations of carbon dots (CDs), doxorubicin (DOX) and CDs-DOX composites in living cells (COS7 and U<sub>2</sub>OS) for the first time. The drug concentration and diffusion coefficient in living cells determined by FCS measurements indicated that the CDs-DOX composite distinctively improved the transmembrane efficiency and rate of drug molecules, in accordance with the conclusions drawn from the fluorescence imaging results. Furthermore, the effects of pH values and ATP concentrations on drug transport across the membrane were also studied. Compared with free DOX under acidic conditions, the CDs-DOX complex has higher cellular uptake and better transmembrane efficacy in U<sub>2</sub>OS cells. Additionally, high concentrations of ATP will cause negative changes in cell membrane permeability, which will hinder the transmembrane transport of CDs and DOX and delay the rapid diffusion of CDs-DOX. The results of this study show that the FCS method can be utilized as a powerful tool for studying the expansion and transport of nanodrugs in living cells, and might provide a new drug exploitation strategy for cancer treatment in vivo.
format	article
author	Xinwei Gao Yanfeng Liu Jia Zhang Luwei Wang Yong Guo Yinru Zhu Zhigang Yang Wei Yan Junle Qu
author_facet	Xinwei Gao Yanfeng Liu Jia Zhang Luwei Wang Yong Guo Yinru Zhu Zhigang Yang Wei Yan Junle Qu
author_sort	Xinwei Gao
title	Nanodrug Transmembrane Transport Research Based on Fluorescence Correlation Spectroscopy
title_short	Nanodrug Transmembrane Transport Research Based on Fluorescence Correlation Spectroscopy
title_full	Nanodrug Transmembrane Transport Research Based on Fluorescence Correlation Spectroscopy
title_fullStr	Nanodrug Transmembrane Transport Research Based on Fluorescence Correlation Spectroscopy
title_full_unstemmed	Nanodrug Transmembrane Transport Research Based on Fluorescence Correlation Spectroscopy
title_sort	nanodrug transmembrane transport research based on fluorescence correlation spectroscopy
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/de694840986a4daab2b80181bc9be4d3
work_keys_str_mv	AT xinweigao nanodrugtransmembranetransportresearchbasedonfluorescencecorrelationspectroscopy AT yanfengliu nanodrugtransmembranetransportresearchbasedonfluorescencecorrelationspectroscopy AT jiazhang nanodrugtransmembranetransportresearchbasedonfluorescencecorrelationspectroscopy AT luweiwang nanodrugtransmembranetransportresearchbasedonfluorescencecorrelationspectroscopy AT yongguo nanodrugtransmembranetransportresearchbasedonfluorescencecorrelationspectroscopy AT yinruzhu nanodrugtransmembranetransportresearchbasedonfluorescencecorrelationspectroscopy AT zhigangyang nanodrugtransmembranetransportresearchbasedonfluorescencecorrelationspectroscopy AT weiyan nanodrugtransmembranetransportresearchbasedonfluorescencecorrelationspectroscopy AT junlequ nanodrugtransmembranetransportresearchbasedonfluorescencecorrelationspectroscopy
_version_	1718411351118839808

Nanodrug Transmembrane Transport Research Based on Fluorescence Correlation Spectroscopy

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