A Cas9 with PAM recognition for adenine dinucleotides
Protospacer adjacent motif (PAM) requirements limit the target range of CRISPR endonucleases. Here, the authors graft the 5$$^{\prime}$$ ′ -NAAN-3$$^{\prime}$$ ′ PAM-interacting domain of SmacCas9 onto SpyCas9 to create adenine dinucleotide targeting chimeras.
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Nature Portfolio
2020
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oai:doaj.org-article:de6c445336ca4aacb34b61cb8daeb3582021-12-02T14:58:44ZA Cas9 with PAM recognition for adenine dinucleotides10.1038/s41467-020-16117-82041-1723https://doaj.org/article/de6c445336ca4aacb34b61cb8daeb3582020-05-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-16117-8https://doaj.org/toc/2041-1723Protospacer adjacent motif (PAM) requirements limit the target range of CRISPR endonucleases. Here, the authors graft the 5$$^{\prime}$$ ′ -NAAN-3$$^{\prime}$$ ′ PAM-interacting domain of SmacCas9 onto SpyCas9 to create adenine dinucleotide targeting chimeras.Pranam ChatterjeeJooyoung LeeLisa NipSabrina R. T. KosekiEmma TysingerErik J. SontheimerJoseph M. JacobsonNoah JakimoNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-6 (2020) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Science Q |
| spellingShingle |
Science Q Pranam Chatterjee Jooyoung Lee Lisa Nip Sabrina R. T. Koseki Emma Tysinger Erik J. Sontheimer Joseph M. Jacobson Noah Jakimo A Cas9 with PAM recognition for adenine dinucleotides |
| description |
Protospacer adjacent motif (PAM) requirements limit the target range of CRISPR endonucleases. Here, the authors graft the 5$$^{\prime}$$ ′ -NAAN-3$$^{\prime}$$ ′ PAM-interacting domain of SmacCas9 onto SpyCas9 to create adenine dinucleotide targeting chimeras. |
| format |
article |
| author |
Pranam Chatterjee Jooyoung Lee Lisa Nip Sabrina R. T. Koseki Emma Tysinger Erik J. Sontheimer Joseph M. Jacobson Noah Jakimo |
| author_facet |
Pranam Chatterjee Jooyoung Lee Lisa Nip Sabrina R. T. Koseki Emma Tysinger Erik J. Sontheimer Joseph M. Jacobson Noah Jakimo |
| author_sort |
Pranam Chatterjee |
| title |
A Cas9 with PAM recognition for adenine dinucleotides |
| title_short |
A Cas9 with PAM recognition for adenine dinucleotides |
| title_full |
A Cas9 with PAM recognition for adenine dinucleotides |
| title_fullStr |
A Cas9 with PAM recognition for adenine dinucleotides |
| title_full_unstemmed |
A Cas9 with PAM recognition for adenine dinucleotides |
| title_sort |
cas9 with pam recognition for adenine dinucleotides |
| publisher |
Nature Portfolio |
| publishDate |
2020 |
| url |
https://doaj.org/article/de6c445336ca4aacb34b61cb8daeb358 |
| work_keys_str_mv |
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| _version_ |
1718389295266398208 |