Electrochemical DNA Sensor Based on Acridine Yellow Adsorbed on Glassy Carbon Electrode

Electrochemical DNA sensors offer unique opportunities for the sensitive detection of specific DNA interactions. In this work, a voltametric DNA sensor is proposed on the base of glassy carbon electrode modified with carbon black, adsorbed acridine yellow and DNA for highly sensitive determination o...

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Autores principales:	Tatjana Kulikova, Anna Porfireva, Alexey Rogov, Gennady Evtugyn
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	electrochemical DNA sensor doxorubicin determination carbon black cyclic voltammetry drug determination Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/de6f9314ec8345e6817d5204fc4e6f5b
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spelling	oai:doaj.org-article:de6f9314ec8345e6817d5204fc4e6f5b2021-11-25T18:59:08ZElectrochemical DNA Sensor Based on Acridine Yellow Adsorbed on Glassy Carbon Electrode10.3390/s212277631424-8220https://doaj.org/article/de6f9314ec8345e6817d5204fc4e6f5b2021-11-01T00:00:00Zhttps://www.mdpi.com/1424-8220/21/22/7763https://doaj.org/toc/1424-8220Electrochemical DNA sensors offer unique opportunities for the sensitive detection of specific DNA interactions. In this work, a voltametric DNA sensor is proposed on the base of glassy carbon electrode modified with carbon black, adsorbed acridine yellow and DNA for highly sensitive determination of doxorubicin antitumor drug. The signal recorded by cyclic voltammetry was attributed to irreversible oxidation of the dye. Its value was altered by aggregation of the hydrophobic dye molecules on the carbon black particles. DNA molecules promote disaggregation of the dye and increased the signal. This effect was partially suppressed by doxorubicin compensate for the charge of DNA in the intercalation. Sensitivity of the signal toward DNA and doxorubicin was additionally increased by treatment of the layer with dimethylformamide. In optimal conditions, the linear range of doxorubicin concentrations determined was 0.1 pM–1.0 nM, and the detection limit was 0.07 pM. No influence of sulfonamide medicines and plasma electrolytes on the doxorubicin determination was shown. The DNA sensor was tested on two medications (doxorubicin-TEVA and doxorubicin-LANS) and showed recoveries of 102–105%. The DNA sensor developed can find applications in the determination of drug residues in blood and for the pharmacokinetics studies.Tatjana KulikovaAnna PorfirevaAlexey RogovGennady EvtugynMDPI AGarticleelectrochemical DNA sensordoxorubicin determinationcarbon blackcyclic voltammetrydrug determinationChemical technologyTP1-1185ENSensors, Vol 21, Iss 7763, p 7763 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	electrochemical DNA sensor doxorubicin determination carbon black cyclic voltammetry drug determination Chemical technology TP1-1185
spellingShingle	electrochemical DNA sensor doxorubicin determination carbon black cyclic voltammetry drug determination Chemical technology TP1-1185 Tatjana Kulikova Anna Porfireva Alexey Rogov Gennady Evtugyn Electrochemical DNA Sensor Based on Acridine Yellow Adsorbed on Glassy Carbon Electrode
description	Electrochemical DNA sensors offer unique opportunities for the sensitive detection of specific DNA interactions. In this work, a voltametric DNA sensor is proposed on the base of glassy carbon electrode modified with carbon black, adsorbed acridine yellow and DNA for highly sensitive determination of doxorubicin antitumor drug. The signal recorded by cyclic voltammetry was attributed to irreversible oxidation of the dye. Its value was altered by aggregation of the hydrophobic dye molecules on the carbon black particles. DNA molecules promote disaggregation of the dye and increased the signal. This effect was partially suppressed by doxorubicin compensate for the charge of DNA in the intercalation. Sensitivity of the signal toward DNA and doxorubicin was additionally increased by treatment of the layer with dimethylformamide. In optimal conditions, the linear range of doxorubicin concentrations determined was 0.1 pM–1.0 nM, and the detection limit was 0.07 pM. No influence of sulfonamide medicines and plasma electrolytes on the doxorubicin determination was shown. The DNA sensor was tested on two medications (doxorubicin-TEVA and doxorubicin-LANS) and showed recoveries of 102–105%. The DNA sensor developed can find applications in the determination of drug residues in blood and for the pharmacokinetics studies.
format	article
author	Tatjana Kulikova Anna Porfireva Alexey Rogov Gennady Evtugyn
author_facet	Tatjana Kulikova Anna Porfireva Alexey Rogov Gennady Evtugyn
author_sort	Tatjana Kulikova
title	Electrochemical DNA Sensor Based on Acridine Yellow Adsorbed on Glassy Carbon Electrode
title_short	Electrochemical DNA Sensor Based on Acridine Yellow Adsorbed on Glassy Carbon Electrode
title_full	Electrochemical DNA Sensor Based on Acridine Yellow Adsorbed on Glassy Carbon Electrode
title_fullStr	Electrochemical DNA Sensor Based on Acridine Yellow Adsorbed on Glassy Carbon Electrode
title_full_unstemmed	Electrochemical DNA Sensor Based on Acridine Yellow Adsorbed on Glassy Carbon Electrode
title_sort	electrochemical dna sensor based on acridine yellow adsorbed on glassy carbon electrode
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/de6f9314ec8345e6817d5204fc4e6f5b
work_keys_str_mv	AT tatjanakulikova electrochemicaldnasensorbasedonacridineyellowadsorbedonglassycarbonelectrode AT annaporfireva electrochemicaldnasensorbasedonacridineyellowadsorbedonglassycarbonelectrode AT alexeyrogov electrochemicaldnasensorbasedonacridineyellowadsorbedonglassycarbonelectrode AT gennadyevtugyn electrochemicaldnasensorbasedonacridineyellowadsorbedonglassycarbonelectrode
_version_	1718410495631818752

Electrochemical DNA Sensor Based on Acridine Yellow Adsorbed on Glassy Carbon Electrode

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