Different Tidal Volumes May Jeopardize Pulmonary Redox and Inflammatory Status in Healthy Rats Undergoing Mechanical Ventilation

Mechanical ventilation (MV) is essential for the treatment of critical patients since it may provide a desired gas exchange. However, MV itself can trigger ventilator-associated lung injury in patients. We hypothesized that the mechanisms of lung injury through redox imbalance might also be associat...

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Autores principales:	Leandro da Silva Cândido, Natália Alves de Matos, Thalles de Freitas Castro, Laisy Cristina de Paula, Aline Maria dos Santos, Guilherme de Paula Costa, André Talvani, Silvia Dantas Cangussú, Walter Araujo Zin, Frank Silva Bezerra
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Cytology QH573-671
Acceso en línea:	https://doaj.org/article/de7214c600f247e5bd66578032cf779e
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spelling	oai:doaj.org-article:de7214c600f247e5bd66578032cf779e2021-11-08T02:35:48ZDifferent Tidal Volumes May Jeopardize Pulmonary Redox and Inflammatory Status in Healthy Rats Undergoing Mechanical Ventilation1942-099410.1155/2021/5196896https://doaj.org/article/de7214c600f247e5bd66578032cf779e2021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/5196896https://doaj.org/toc/1942-0994Mechanical ventilation (MV) is essential for the treatment of critical patients since it may provide a desired gas exchange. However, MV itself can trigger ventilator-associated lung injury in patients. We hypothesized that the mechanisms of lung injury through redox imbalance might also be associated with pulmonary inflammatory status, which has not been so far described. We tested it by delivering different tidal volumes to normal lungs undergoing MV. Healthy Wistar rats were divided into spontaneously breathing animals (control group, CG), and rats were submitted to MV (controlled ventilation mode) with tidal volumes of 4 mL/kg (MVG4), 8 mL/kg (MVG8), or 12 mL/kg (MVG12), zero end-expiratory pressure (ZEEP), and normoxia (FiO2=21%) for 1 hour. After ventilation and euthanasia, arterial blood, bronchoalveolar lavage fluid (BALF), and lungs were collected for subsequent analysis. MVG12 presented lower PaCO2 and bicarbonate content in the arterial blood than CG, MVG4, and MVG8. Neutrophil influx in BALF and MPO activity in lung tissue homogenate were significantly higher in MVG12 than in CG. The levels of CCL5, TNF-α, IL-1, and IL-6 in lung tissue homogenate were higher in MVG12 than in CG and MVG4. In the lung parenchyma, the lipid peroxidation was more important in MVG12 than in CG, MVG4, and MVG8, while there was more protein oxidation in MVG12 than in CG and MVG4. The stereological analysis confirmed the histological pulmonary changes in MVG12. The association of controlled mode ventilation and high tidal volume, without PEEP and normoxia, impaired pulmonary histoarchitecture and triggered redox imbalance and lung inflammation in healthy adult rats.Leandro da Silva CândidoNatália Alves de MatosThalles de Freitas CastroLaisy Cristina de PaulaAline Maria dos SantosGuilherme de Paula CostaAndré TalvaniSilvia Dantas CangussúWalter Araujo ZinFrank Silva BezerraHindawi LimitedarticleCytologyQH573-671ENOxidative Medicine and Cellular Longevity, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cytology QH573-671
spellingShingle	Cytology QH573-671 Leandro da Silva Cândido Natália Alves de Matos Thalles de Freitas Castro Laisy Cristina de Paula Aline Maria dos Santos Guilherme de Paula Costa André Talvani Silvia Dantas Cangussú Walter Araujo Zin Frank Silva Bezerra Different Tidal Volumes May Jeopardize Pulmonary Redox and Inflammatory Status in Healthy Rats Undergoing Mechanical Ventilation
description	Mechanical ventilation (MV) is essential for the treatment of critical patients since it may provide a desired gas exchange. However, MV itself can trigger ventilator-associated lung injury in patients. We hypothesized that the mechanisms of lung injury through redox imbalance might also be associated with pulmonary inflammatory status, which has not been so far described. We tested it by delivering different tidal volumes to normal lungs undergoing MV. Healthy Wistar rats were divided into spontaneously breathing animals (control group, CG), and rats were submitted to MV (controlled ventilation mode) with tidal volumes of 4 mL/kg (MVG4), 8 mL/kg (MVG8), or 12 mL/kg (MVG12), zero end-expiratory pressure (ZEEP), and normoxia (FiO2=21%) for 1 hour. After ventilation and euthanasia, arterial blood, bronchoalveolar lavage fluid (BALF), and lungs were collected for subsequent analysis. MVG12 presented lower PaCO2 and bicarbonate content in the arterial blood than CG, MVG4, and MVG8. Neutrophil influx in BALF and MPO activity in lung tissue homogenate were significantly higher in MVG12 than in CG. The levels of CCL5, TNF-α, IL-1, and IL-6 in lung tissue homogenate were higher in MVG12 than in CG and MVG4. In the lung parenchyma, the lipid peroxidation was more important in MVG12 than in CG, MVG4, and MVG8, while there was more protein oxidation in MVG12 than in CG and MVG4. The stereological analysis confirmed the histological pulmonary changes in MVG12. The association of controlled mode ventilation and high tidal volume, without PEEP and normoxia, impaired pulmonary histoarchitecture and triggered redox imbalance and lung inflammation in healthy adult rats.
format	article
author	Leandro da Silva Cândido Natália Alves de Matos Thalles de Freitas Castro Laisy Cristina de Paula Aline Maria dos Santos Guilherme de Paula Costa André Talvani Silvia Dantas Cangussú Walter Araujo Zin Frank Silva Bezerra
author_facet	Leandro da Silva Cândido Natália Alves de Matos Thalles de Freitas Castro Laisy Cristina de Paula Aline Maria dos Santos Guilherme de Paula Costa André Talvani Silvia Dantas Cangussú Walter Araujo Zin Frank Silva Bezerra
author_sort	Leandro da Silva Cândido
title	Different Tidal Volumes May Jeopardize Pulmonary Redox and Inflammatory Status in Healthy Rats Undergoing Mechanical Ventilation
title_short	Different Tidal Volumes May Jeopardize Pulmonary Redox and Inflammatory Status in Healthy Rats Undergoing Mechanical Ventilation
title_full	Different Tidal Volumes May Jeopardize Pulmonary Redox and Inflammatory Status in Healthy Rats Undergoing Mechanical Ventilation
title_fullStr	Different Tidal Volumes May Jeopardize Pulmonary Redox and Inflammatory Status in Healthy Rats Undergoing Mechanical Ventilation
title_full_unstemmed	Different Tidal Volumes May Jeopardize Pulmonary Redox and Inflammatory Status in Healthy Rats Undergoing Mechanical Ventilation
title_sort	different tidal volumes may jeopardize pulmonary redox and inflammatory status in healthy rats undergoing mechanical ventilation
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/de7214c600f247e5bd66578032cf779e
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Different Tidal Volumes May Jeopardize Pulmonary Redox and Inflammatory Status in Healthy Rats Undergoing Mechanical Ventilation

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