Molecular identification of adult and juvenile linyphiid and theridiid spiders in Alpine glacier foreland communities.

In glacier forelands spiders constitute a large proportion of the invertebrate community. Therefore, it is important to be able to determine the species that can be found in these areas. Linyphiid and theridiid spider identification is currently not possible in juvenile specimens using traditional m...

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Autores principales:	Lorna Raso, Daniela Sint, Alexander Rief, Rüdiger Kaufmann, Michael Traugott
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/de77f7f83330449180bfe4ba32046758
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spelling	oai:doaj.org-article:de77f7f83330449180bfe4ba320467582021-11-25T06:07:38ZMolecular identification of adult and juvenile linyphiid and theridiid spiders in Alpine glacier foreland communities.1932-620310.1371/journal.pone.0101755https://doaj.org/article/de77f7f83330449180bfe4ba320467582014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25050841/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203In glacier forelands spiders constitute a large proportion of the invertebrate community. Therefore, it is important to be able to determine the species that can be found in these areas. Linyphiid and theridiid spider identification is currently not possible in juvenile specimens using traditional morphological based methods, however, a large proportion of the population in these areas are usually juveniles. Molecular methods permit identification of species at different life stages, making juvenile identification possible. In this study we tested a molecular tool to identify the 10 most common species of Linyphiidae and Theridiidae found in three glacier foreland communities of the Austrian Alps. Two multiplex PCR systems were developed and over 90% of the 753 field-collected spiders were identified successfully. The species targeted were found to be common in all three valleys during the summer of 2010. A comparison between the molecular and morphological data showed that although there was a slight difference in the results, the overall outcome was the same independently of the identification method used. We believe the quick and reliable identification of the spiders via the multiplex PCR assays developed here will aid the study of these families in Alpine habitats.Lorna RasoDaniela SintAlexander RiefRüdiger KaufmannMichael TraugottPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 7, p e101755 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lorna Raso Daniela Sint Alexander Rief Rüdiger Kaufmann Michael Traugott Molecular identification of adult and juvenile linyphiid and theridiid spiders in Alpine glacier foreland communities.
description	In glacier forelands spiders constitute a large proportion of the invertebrate community. Therefore, it is important to be able to determine the species that can be found in these areas. Linyphiid and theridiid spider identification is currently not possible in juvenile specimens using traditional morphological based methods, however, a large proportion of the population in these areas are usually juveniles. Molecular methods permit identification of species at different life stages, making juvenile identification possible. In this study we tested a molecular tool to identify the 10 most common species of Linyphiidae and Theridiidae found in three glacier foreland communities of the Austrian Alps. Two multiplex PCR systems were developed and over 90% of the 753 field-collected spiders were identified successfully. The species targeted were found to be common in all three valleys during the summer of 2010. A comparison between the molecular and morphological data showed that although there was a slight difference in the results, the overall outcome was the same independently of the identification method used. We believe the quick and reliable identification of the spiders via the multiplex PCR assays developed here will aid the study of these families in Alpine habitats.
format	article
author	Lorna Raso Daniela Sint Alexander Rief Rüdiger Kaufmann Michael Traugott
author_facet	Lorna Raso Daniela Sint Alexander Rief Rüdiger Kaufmann Michael Traugott
author_sort	Lorna Raso
title	Molecular identification of adult and juvenile linyphiid and theridiid spiders in Alpine glacier foreland communities.
title_short	Molecular identification of adult and juvenile linyphiid and theridiid spiders in Alpine glacier foreland communities.
title_full	Molecular identification of adult and juvenile linyphiid and theridiid spiders in Alpine glacier foreland communities.
title_fullStr	Molecular identification of adult and juvenile linyphiid and theridiid spiders in Alpine glacier foreland communities.
title_full_unstemmed	Molecular identification of adult and juvenile linyphiid and theridiid spiders in Alpine glacier foreland communities.
title_sort	molecular identification of adult and juvenile linyphiid and theridiid spiders in alpine glacier foreland communities.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/de77f7f83330449180bfe4ba32046758
work_keys_str_mv	AT lornaraso molecularidentificationofadultandjuvenilelinyphiidandtheridiidspidersinalpineglacierforelandcommunities AT danielasint molecularidentificationofadultandjuvenilelinyphiidandtheridiidspidersinalpineglacierforelandcommunities AT alexanderrief molecularidentificationofadultandjuvenilelinyphiidandtheridiidspidersinalpineglacierforelandcommunities AT rudigerkaufmann molecularidentificationofadultandjuvenilelinyphiidandtheridiidspidersinalpineglacierforelandcommunities AT michaeltraugott molecularidentificationofadultandjuvenilelinyphiidandtheridiidspidersinalpineglacierforelandcommunities
_version_	1718414194761531392

Molecular identification of adult and juvenile linyphiid and theridiid spiders in Alpine glacier foreland communities.

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