Flavonoids of Rosa rugosa Thunb. inhibit tumor proliferation and metastasis in human hepatocellular carcinoma HepG2 cells

Rosa rugosa Thunb. is recognized as both medicine and edible in China. The article investigated the antitumor activity of rose flavonoids. Water-extracted rose flavonoids (RFW) and ethanol-extracted rose flavonoids (RFE) were achieved by extracting with distilled water and 70% ethanol, respectively....

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Autores principales:	Zhiguo Zhang, Meilin Wang, Shu Xing, Chen Zhang
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2022
Materias:	Rose flavonoids Tumor metastasis Apoptosis Human liver cancer Nutrition. Foods and food supply TX341-641
Acceso en línea:	https://doaj.org/article/de7c4bab324f465e800bc93526feebdd
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spelling	oai:doaj.org-article:de7c4bab324f465e800bc93526feebdd2021-11-28T04:32:07ZFlavonoids of Rosa rugosa Thunb. inhibit tumor proliferation and metastasis in human hepatocellular carcinoma HepG2 cells2213-453010.1016/j.fshw.2021.11.016https://doaj.org/article/de7c4bab324f465e800bc93526feebdd2022-03-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2213453021001129https://doaj.org/toc/2213-4530Rosa rugosa Thunb. is recognized as both medicine and edible in China. The article investigated the antitumor activity of rose flavonoids. Water-extracted rose flavonoids (RFW) and ethanol-extracted rose flavonoids (RFE) were achieved by extracting with distilled water and 70% ethanol, respectively. The effects of the two extracts on proliferation inhibition, apoptosis inducement and metastasis prevention of human HepG2 hepatocellular carcinoma cell lines were tested, via optical/fluorescence microscopy, MTT detection, Transwell assay, flow cytometry and Western blot, etc. The results indicated that rose flavonoids at low concentration (10–40 μg/mL) had a better inhibitory effect on migration and invasion of HepG2 cells in a dose-dependent manner, while rose flavonoids at high concentration (80–160 μg/mL) could induce apoptosis of HepG2 cells by up-regulating the expression of pro-apoptotic proteins p53 and Bax, and down-regulating the expression of anti-apoptotic proteins Bcl-2, leading to the functioning of caspase-3 and caspase-9. The effect of RFE at the same concentration was significantly better than that of RFW. Conclusion, this study found that rose flavonoids had a certain inhibitory effect on proliferation and metastasis of human liver cancer cells HepG2, indicating the application of rose flavonoids in preventing and treating of liver cancer.Zhiguo ZhangMeilin WangShu XingChen ZhangKeAi Communications Co., Ltd.articleRose flavonoidsTumor metastasisApoptosisHuman liver cancerNutrition. Foods and food supplyTX341-641ENFood Science and Human Wellness, Vol 11, Iss 2, Pp 374-382 (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Rose flavonoids Tumor metastasis Apoptosis Human liver cancer Nutrition. Foods and food supply TX341-641
spellingShingle	Rose flavonoids Tumor metastasis Apoptosis Human liver cancer Nutrition. Foods and food supply TX341-641 Zhiguo Zhang Meilin Wang Shu Xing Chen Zhang Flavonoids of Rosa rugosa Thunb. inhibit tumor proliferation and metastasis in human hepatocellular carcinoma HepG2 cells
description	Rosa rugosa Thunb. is recognized as both medicine and edible in China. The article investigated the antitumor activity of rose flavonoids. Water-extracted rose flavonoids (RFW) and ethanol-extracted rose flavonoids (RFE) were achieved by extracting with distilled water and 70% ethanol, respectively. The effects of the two extracts on proliferation inhibition, apoptosis inducement and metastasis prevention of human HepG2 hepatocellular carcinoma cell lines were tested, via optical/fluorescence microscopy, MTT detection, Transwell assay, flow cytometry and Western blot, etc. The results indicated that rose flavonoids at low concentration (10–40 μg/mL) had a better inhibitory effect on migration and invasion of HepG2 cells in a dose-dependent manner, while rose flavonoids at high concentration (80–160 μg/mL) could induce apoptosis of HepG2 cells by up-regulating the expression of pro-apoptotic proteins p53 and Bax, and down-regulating the expression of anti-apoptotic proteins Bcl-2, leading to the functioning of caspase-3 and caspase-9. The effect of RFE at the same concentration was significantly better than that of RFW. Conclusion, this study found that rose flavonoids had a certain inhibitory effect on proliferation and metastasis of human liver cancer cells HepG2, indicating the application of rose flavonoids in preventing and treating of liver cancer.
format	article
author	Zhiguo Zhang Meilin Wang Shu Xing Chen Zhang
author_facet	Zhiguo Zhang Meilin Wang Shu Xing Chen Zhang
author_sort	Zhiguo Zhang
title	Flavonoids of Rosa rugosa Thunb. inhibit tumor proliferation and metastasis in human hepatocellular carcinoma HepG2 cells
title_short	Flavonoids of Rosa rugosa Thunb. inhibit tumor proliferation and metastasis in human hepatocellular carcinoma HepG2 cells
title_full	Flavonoids of Rosa rugosa Thunb. inhibit tumor proliferation and metastasis in human hepatocellular carcinoma HepG2 cells
title_fullStr	Flavonoids of Rosa rugosa Thunb. inhibit tumor proliferation and metastasis in human hepatocellular carcinoma HepG2 cells
title_full_unstemmed	Flavonoids of Rosa rugosa Thunb. inhibit tumor proliferation and metastasis in human hepatocellular carcinoma HepG2 cells
title_sort	flavonoids of rosa rugosa thunb. inhibit tumor proliferation and metastasis in human hepatocellular carcinoma hepg2 cells
publisher	KeAi Communications Co., Ltd.
publishDate	2022
url	https://doaj.org/article/de7c4bab324f465e800bc93526feebdd
work_keys_str_mv	AT zhiguozhang flavonoidsofrosarugosathunbinhibittumorproliferationandmetastasisinhumanhepatocellularcarcinomahepg2cells AT meilinwang flavonoidsofrosarugosathunbinhibittumorproliferationandmetastasisinhumanhepatocellularcarcinomahepg2cells AT shuxing flavonoidsofrosarugosathunbinhibittumorproliferationandmetastasisinhumanhepatocellularcarcinomahepg2cells AT chenzhang flavonoidsofrosarugosathunbinhibittumorproliferationandmetastasisinhumanhepatocellularcarcinomahepg2cells
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Flavonoids of Rosa rugosa Thunb. inhibit tumor proliferation and metastasis in human hepatocellular carcinoma HepG2 cells

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