Iron (II) and other heavy-metal tolerance in bacteria isolated from rock varnish in the arid region of Al-Jafer Basin, Jordan

Alnaimat S, Shattal SA, AlthunibatO, Alsbou E, Amasha R. 2017. Iron (II) and other heavy-metal tolerance in bacteria isolated from rock varnish in the arid region of Al-Jafer Basin, Jordan. Biodiversitas 18: 1250-1257. This study deals with enumeration, identification and molecular characterization...

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Autores principales:	SULAIMAN ALNAIMAT, SAQER ABU SHATTAL, OSAMA ALTHUNIBAT, EID ALSBOU, REDA AMASHA
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2017
Materias:	al-jafer basin rock varnish bacteria tolerant of heavy metals 16s rdna Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/de800725a1244f26bd1b07bd5d4c81b1
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spelling	oai:doaj.org-article:de800725a1244f26bd1b07bd5d4c81b12021-11-16T13:56:34ZIron (II) and other heavy-metal tolerance in bacteria isolated from rock varnish in the arid region of Al-Jafer Basin, Jordan1412-033X2085-472210.13057/biodiv/d180350https://doaj.org/article/de800725a1244f26bd1b07bd5d4c81b12017-07-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2050https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Alnaimat S, Shattal SA, AlthunibatO, Alsbou E, Amasha R. 2017. Iron (II) and other heavy-metal tolerance in bacteria isolated from rock varnish in the arid region of Al-Jafer Basin, Jordan. Biodiversitas 18: 1250-1257. This study deals with enumeration, identification and molecular characterization of bacterial species tolerant of iron (II) and other heavy metals that isolated from rock varnish collected from the arid region of Al-Jafer Basin, Jordan. Based on conventional culture-dependent methods, six isolates exhibited a high degree of tolerance to iron (II) and other heavy metals with a minimum inhibitory concentration (550â€“600 mg/L) to iron, (500-550 mg/L) to lead, (350 mg/L) to copper, (400-450 mg/L) to chromium, (150-200 mg/L) to silver, and (150-200 mg/L) to mercury, in solid media. The results showed that all isolates could tolerate 2000 mg/L Fe2+ expressed as maximum tolerance concentration (MTC). The multiple metal resistances of these isolates were also associated with multiple antibiotic resistances. These iron tolerant isolates were shown to be capable of growth at temperatures ranging from 30 to 40Â°C and pH values ranging from 5 to 9 with some differences between the six isolates. On the basis of morphology and of 16S rDNA gene sequencing and phylogeny analysis, the iron-tolerant isolates were identified as Bacillus zhangzhouensis (DVCr1), Staphylococcus warneri (DVCr6), Bacillus cereus (DVCr7), Staphylococcus pasteuri (DVCu2), Brevibacterium frigoritolerans (DVPb) and Bacillus altitudinis (DVCr10). The 16S rDNA sequences from five of the six strains were submitted to GenBank and are available under the accession numbers KX781139, KX781140, KX781141, KX781145 and KX781148. The results highlight the high potential of a non-polluted habitat like rock varnish to provide new microbial communities that could be used for enhanced bioremediation of heavy metals from a contaminated site.SULAIMAN ALNAIMATSAQER ABU SHATTALOSAMA ALTHUNIBATEID ALSBOUREDA AMASHAMBI & UNS Soloarticleal-jafer basinrock varnishbacteria tolerant of heavy metals16s rdnaBiology (General)QH301-705.5ENBiodiversitas, Vol 18, Iss 3, Pp 1250-1257 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	al-jafer basin rock varnish bacteria tolerant of heavy metals 16s rdna Biology (General) QH301-705.5
spellingShingle	al-jafer basin rock varnish bacteria tolerant of heavy metals 16s rdna Biology (General) QH301-705.5 SULAIMAN ALNAIMAT SAQER ABU SHATTAL OSAMA ALTHUNIBAT EID ALSBOU REDA AMASHA Iron (II) and other heavy-metal tolerance in bacteria isolated from rock varnish in the arid region of Al-Jafer Basin, Jordan
description	Alnaimat S, Shattal SA, AlthunibatO, Alsbou E, Amasha R. 2017. Iron (II) and other heavy-metal tolerance in bacteria isolated from rock varnish in the arid region of Al-Jafer Basin, Jordan. Biodiversitas 18: 1250-1257. This study deals with enumeration, identification and molecular characterization of bacterial species tolerant of iron (II) and other heavy metals that isolated from rock varnish collected from the arid region of Al-Jafer Basin, Jordan. Based on conventional culture-dependent methods, six isolates exhibited a high degree of tolerance to iron (II) and other heavy metals with a minimum inhibitory concentration (550â€“600 mg/L) to iron, (500-550 mg/L) to lead, (350 mg/L) to copper, (400-450 mg/L) to chromium, (150-200 mg/L) to silver, and (150-200 mg/L) to mercury, in solid media. The results showed that all isolates could tolerate 2000 mg/L Fe2+ expressed as maximum tolerance concentration (MTC). The multiple metal resistances of these isolates were also associated with multiple antibiotic resistances. These iron tolerant isolates were shown to be capable of growth at temperatures ranging from 30 to 40Â°C and pH values ranging from 5 to 9 with some differences between the six isolates. On the basis of morphology and of 16S rDNA gene sequencing and phylogeny analysis, the iron-tolerant isolates were identified as Bacillus zhangzhouensis (DVCr1), Staphylococcus warneri (DVCr6), Bacillus cereus (DVCr7), Staphylococcus pasteuri (DVCu2), Brevibacterium frigoritolerans (DVPb) and Bacillus altitudinis (DVCr10). The 16S rDNA sequences from five of the six strains were submitted to GenBank and are available under the accession numbers KX781139, KX781140, KX781141, KX781145 and KX781148. The results highlight the high potential of a non-polluted habitat like rock varnish to provide new microbial communities that could be used for enhanced bioremediation of heavy metals from a contaminated site.
format	article
author	SULAIMAN ALNAIMAT SAQER ABU SHATTAL OSAMA ALTHUNIBAT EID ALSBOU REDA AMASHA
author_facet	SULAIMAN ALNAIMAT SAQER ABU SHATTAL OSAMA ALTHUNIBAT EID ALSBOU REDA AMASHA
author_sort	SULAIMAN ALNAIMAT
title	Iron (II) and other heavy-metal tolerance in bacteria isolated from rock varnish in the arid region of Al-Jafer Basin, Jordan
title_short	Iron (II) and other heavy-metal tolerance in bacteria isolated from rock varnish in the arid region of Al-Jafer Basin, Jordan
title_full	Iron (II) and other heavy-metal tolerance in bacteria isolated from rock varnish in the arid region of Al-Jafer Basin, Jordan
title_fullStr	Iron (II) and other heavy-metal tolerance in bacteria isolated from rock varnish in the arid region of Al-Jafer Basin, Jordan
title_full_unstemmed	Iron (II) and other heavy-metal tolerance in bacteria isolated from rock varnish in the arid region of Al-Jafer Basin, Jordan
title_sort	iron (ii) and other heavy-metal tolerance in bacteria isolated from rock varnish in the arid region of al-jafer basin, jordan
publisher	MBI & UNS Solo
publishDate	2017
url	https://doaj.org/article/de800725a1244f26bd1b07bd5d4c81b1
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Iron (II) and other heavy-metal tolerance in bacteria isolated from rock varnish in the arid region of Al-Jafer Basin, Jordan

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