Imaging multicellular specimens with real-time optimized tiling light-sheet selective plane illumination microscopy

Selective plane illumination microscopy (SPIM) is capable of high-resolution, high-speed 3D imaging of single cells, but application to multicellular samples is challenging. Here the authors develop tiling light sheet SPIM to image large multicellular specimens in 3D with subcellular resolution.

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Detalles Bibliográficos
Autores principales: Qinyi Fu, Benjamin L. Martin, David Q. Matus, Liang Gao
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2016
Materias:
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Acceso en línea:https://doaj.org/article/de880dcbf6ca4d62ad998fdf43d78545
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Descripción
Sumario:Selective plane illumination microscopy (SPIM) is capable of high-resolution, high-speed 3D imaging of single cells, but application to multicellular samples is challenging. Here the authors develop tiling light sheet SPIM to image large multicellular specimens in 3D with subcellular resolution.