Imaging multicellular specimens with real-time optimized tiling light-sheet selective plane illumination microscopy

Selective plane illumination microscopy (SPIM) is capable of high-resolution, high-speed 3D imaging of single cells, but application to multicellular samples is challenging. Here the authors develop tiling light sheet SPIM to image large multicellular specimens in 3D with subcellular resolution.

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Autores principales: Qinyi Fu, Benjamin L. Martin, David Q. Matus, Liang Gao
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2016
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Acceso en línea:https://doaj.org/article/de880dcbf6ca4d62ad998fdf43d78545
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spelling oai:doaj.org-article:de880dcbf6ca4d62ad998fdf43d785452021-12-02T14:38:55ZImaging multicellular specimens with real-time optimized tiling light-sheet selective plane illumination microscopy10.1038/ncomms110882041-1723https://doaj.org/article/de880dcbf6ca4d62ad998fdf43d785452016-03-01T00:00:00Zhttps://doi.org/10.1038/ncomms11088https://doaj.org/toc/2041-1723Selective plane illumination microscopy (SPIM) is capable of high-resolution, high-speed 3D imaging of single cells, but application to multicellular samples is challenging. Here the authors develop tiling light sheet SPIM to image large multicellular specimens in 3D with subcellular resolution.Qinyi FuBenjamin L. MartinDavid Q. MatusLiang GaoNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-10 (2016)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Qinyi Fu
Benjamin L. Martin
David Q. Matus
Liang Gao
Imaging multicellular specimens with real-time optimized tiling light-sheet selective plane illumination microscopy
description Selective plane illumination microscopy (SPIM) is capable of high-resolution, high-speed 3D imaging of single cells, but application to multicellular samples is challenging. Here the authors develop tiling light sheet SPIM to image large multicellular specimens in 3D with subcellular resolution.
format article
author Qinyi Fu
Benjamin L. Martin
David Q. Matus
Liang Gao
author_facet Qinyi Fu
Benjamin L. Martin
David Q. Matus
Liang Gao
author_sort Qinyi Fu
title Imaging multicellular specimens with real-time optimized tiling light-sheet selective plane illumination microscopy
title_short Imaging multicellular specimens with real-time optimized tiling light-sheet selective plane illumination microscopy
title_full Imaging multicellular specimens with real-time optimized tiling light-sheet selective plane illumination microscopy
title_fullStr Imaging multicellular specimens with real-time optimized tiling light-sheet selective plane illumination microscopy
title_full_unstemmed Imaging multicellular specimens with real-time optimized tiling light-sheet selective plane illumination microscopy
title_sort imaging multicellular specimens with real-time optimized tiling light-sheet selective plane illumination microscopy
publisher Nature Portfolio
publishDate 2016
url https://doaj.org/article/de880dcbf6ca4d62ad998fdf43d78545
work_keys_str_mv AT qinyifu imagingmulticellularspecimenswithrealtimeoptimizedtilinglightsheetselectiveplaneilluminationmicroscopy
AT benjaminlmartin imagingmulticellularspecimenswithrealtimeoptimizedtilinglightsheetselectiveplaneilluminationmicroscopy
AT davidqmatus imagingmulticellularspecimenswithrealtimeoptimizedtilinglightsheetselectiveplaneilluminationmicroscopy
AT lianggao imagingmulticellularspecimenswithrealtimeoptimizedtilinglightsheetselectiveplaneilluminationmicroscopy
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