Biomass Extract of Ipomea carnea and Its Inhibition against Trichoderma asperellum

This study aims to determine the type of metabolites contained in the extraction of Ipomea carnea canopy biomass and its inhibitory effect in vitro against Trichoderma asperellum Tc-Jjr-02 which has been tested as an active ingredient in bio fungicides and biofertilizers. The initial stage of the re...

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Autores principales:	Sutarman, Miftahurrohmat A., Prihatiningrum AE., Arifin S.
Formato:	article
Lenguaje:	EN FR
Publicado:	EDP Sciences 2021
Materias:	Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/de977a6bc2ba4bd38f7ea6565aab3e77
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spelling	oai:doaj.org-article:de977a6bc2ba4bd38f7ea6565aab3e772021-11-08T15:19:28ZBiomass Extract of Ipomea carnea and Its Inhibition against Trichoderma asperellum2267-124210.1051/e3sconf/202131603011https://doaj.org/article/de977a6bc2ba4bd38f7ea6565aab3e772021-01-01T00:00:00Zhttps://www.e3s-conferences.org/articles/e3sconf/pdf/2021/92/e3sconf_iconard2021_03011.pdfhttps://doaj.org/toc/2267-1242This study aims to determine the type of metabolites contained in the extraction of Ipomea carnea canopy biomass and its inhibitory effect in vitro against Trichoderma asperellum Tc-Jjr-02 which has been tested as an active ingredient in bio fungicides and biofertilizers. The initial stage of the research is the determination of the metabolites contained in the extract with methanol solvent through Gas Chromatography-Mass Spectrophotometry (GCMS) analysis. The next step was an in vitro test of the extract's inhibitory power to the growth of T. asperellum fungal colonies during the incubation period of up to 96 hours. The results of GCMS analysis of extracts with methanol solvents obtained 24 types of metabolites, some of which can inhibit fungal growth. Crude extract of canopy biomass of I. carnea at a concentration of 2% inhibited the growth of all Trichoderma isolates by 20.97±3.36% (Tc-Jjr-02), 38.15±32.59% (Tc-Sba-01), and 45.30±1.86% (Tc-Jro-01), while at a concentration of 20% inhibited isolates Tc-Sba-01 and Tc-Jro-01 were 35.19±4.90% and 42.59±1.85% in vitro at 96 hours after inoculation, respectively. The isolate of T. esperellum Tc-Jjr-02 has the potential to be formulated with I. carnea biomass as compost in the future.SutarmanMiftahurrohmat A.Prihatiningrum AE.Arifin S.EDP SciencesarticleEnvironmental sciencesGE1-350ENFRE3S Web of Conferences, Vol 316, p 03011 (2021)
institution	DOAJ
collection	DOAJ
language	EN FR
topic	Environmental sciences GE1-350
spellingShingle	Environmental sciences GE1-350 Sutarman Miftahurrohmat A. Prihatiningrum AE. Arifin S. Biomass Extract of Ipomea carnea and Its Inhibition against Trichoderma asperellum
description	This study aims to determine the type of metabolites contained in the extraction of Ipomea carnea canopy biomass and its inhibitory effect in vitro against Trichoderma asperellum Tc-Jjr-02 which has been tested as an active ingredient in bio fungicides and biofertilizers. The initial stage of the research is the determination of the metabolites contained in the extract with methanol solvent through Gas Chromatography-Mass Spectrophotometry (GCMS) analysis. The next step was an in vitro test of the extract's inhibitory power to the growth of T. asperellum fungal colonies during the incubation period of up to 96 hours. The results of GCMS analysis of extracts with methanol solvents obtained 24 types of metabolites, some of which can inhibit fungal growth. Crude extract of canopy biomass of I. carnea at a concentration of 2% inhibited the growth of all Trichoderma isolates by 20.97±3.36% (Tc-Jjr-02), 38.15±32.59% (Tc-Sba-01), and 45.30±1.86% (Tc-Jro-01), while at a concentration of 20% inhibited isolates Tc-Sba-01 and Tc-Jro-01 were 35.19±4.90% and 42.59±1.85% in vitro at 96 hours after inoculation, respectively. The isolate of T. esperellum Tc-Jjr-02 has the potential to be formulated with I. carnea biomass as compost in the future.
format	article
author	Sutarman Miftahurrohmat A. Prihatiningrum AE. Arifin S.
author_facet	Sutarman Miftahurrohmat A. Prihatiningrum AE. Arifin S.
author_sort	Sutarman
title	Biomass Extract of Ipomea carnea and Its Inhibition against Trichoderma asperellum
title_short	Biomass Extract of Ipomea carnea and Its Inhibition against Trichoderma asperellum
title_full	Biomass Extract of Ipomea carnea and Its Inhibition against Trichoderma asperellum
title_fullStr	Biomass Extract of Ipomea carnea and Its Inhibition against Trichoderma asperellum
title_full_unstemmed	Biomass Extract of Ipomea carnea and Its Inhibition against Trichoderma asperellum
title_sort	biomass extract of ipomea carnea and its inhibition against trichoderma asperellum
publisher	EDP Sciences
publishDate	2021
url	https://doaj.org/article/de977a6bc2ba4bd38f7ea6565aab3e77
work_keys_str_mv	AT sutarman biomassextractofipomeacarneaanditsinhibitionagainsttrichodermaasperellum AT miftahurrohmata biomassextractofipomeacarneaanditsinhibitionagainsttrichodermaasperellum AT prihatiningrumae biomassextractofipomeacarneaanditsinhibitionagainsttrichodermaasperellum AT arifins biomassextractofipomeacarneaanditsinhibitionagainsttrichodermaasperellum
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Biomass Extract of Ipomea carnea and Its Inhibition against Trichoderma asperellum

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