Hepatoprotective Constituents of Total Dibenzocyclooctadiene Lignans from <i>Schisandra chinensis</i> Based on the Spectrum-Effect Relationship

To scientifically clarify the hepatoprotective constituents of Fructus Schizandrae chinensis, eleven batches samples of total dibenzocyclooctadiene lignans (TDL) from <i>Schisandra chinensis</i> were prepared by using the optimum extraction technique. Characteristic high-performance liqu...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lu-Lu Deng, Xu-Dong Xie, Jiang Li, Dao-Ping Wang, Xiao-Jiang Hao, Gang Chen, Shu-Zhen Mu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Schisandra chinensis</i> total dibenzocyclooctadiene lignans (TDL) high-performance liquid chromatography (HPLC) fingerprint hepatoprotective effects spectrum-effect relationship isolation and purification Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/de97d641938f479abf1b38d758bbfac5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:de97d641938f479abf1b38d758bbfac5
record_format	dspace
spelling	oai:doaj.org-article:de97d641938f479abf1b38d758bbfac52021-11-11T18:32:15ZHepatoprotective Constituents of Total Dibenzocyclooctadiene Lignans from <i>Schisandra chinensis</i> Based on the Spectrum-Effect Relationship10.3390/molecules262165541420-3049https://doaj.org/article/de97d641938f479abf1b38d758bbfac52021-10-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6554https://doaj.org/toc/1420-3049To scientifically clarify the hepatoprotective constituents of Fructus Schizandrae chinensis, eleven batches samples of total dibenzocyclooctadiene lignans (TDL) from <i>Schisandra chinensis</i> were prepared by using the optimum extraction technique. Characteristic high-performance liquid chromatography (HPLC) chromatograms were obtained through HPLC analysis technology, and the hepatoprotective effects of the eleven batches of TDL were evaluated by MTT assay. Based on the chemical and biological activity results, the spectrum-effect relationship between the characteristic HPLC fingerprints and the hepatoprotective effect of TDL was established using Minitab 16.0 data analysis software. On the basis of the spectrum-effect relationship, thirteen compounds (<b>1</b>–<b>13</b>) were obtained from the TDL by chemical natural product chemical separation and purification technology, and their structures were identified on the basis of the spectral data and the literature. Based on these compounds, thirteen common peaks among the thirty-three chromatographic peaks in the above HPLC fingerprints were identified. Our findings showed that some components, including, schisandrin B (<b>2</b>), schisandrin A (<b>3</b>), and schisandrol B (<b>7</b>) had significant roles in promoting hepatoprotective activity. Preliminary verification of the spectrum-effect relationship of TDL from <i>S. chinensis</i> was carried out, and the results confirmed that the activity of a composite of these three key components in optimal ratios was better than that of any individual compound, which potentially confirmed the reliability of the spectrum-effect relationship and the synergistic effects of traditional Chinese medicine.Lu-Lu DengXu-Dong XieJiang LiDao-Ping WangXiao-Jiang HaoGang ChenShu-Zhen MuMDPI AGarticle<i>Schisandra chinensis</i>total dibenzocyclooctadiene lignans (TDL)high-performance liquid chromatography (HPLC) fingerprinthepatoprotective effectsspectrum-effect relationshipisolation and purificationOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6554, p 6554 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Schisandra chinensis</i> total dibenzocyclooctadiene lignans (TDL) high-performance liquid chromatography (HPLC) fingerprint hepatoprotective effects spectrum-effect relationship isolation and purification Organic chemistry QD241-441
spellingShingle	<i>Schisandra chinensis</i> total dibenzocyclooctadiene lignans (TDL) high-performance liquid chromatography (HPLC) fingerprint hepatoprotective effects spectrum-effect relationship isolation and purification Organic chemistry QD241-441 Lu-Lu Deng Xu-Dong Xie Jiang Li Dao-Ping Wang Xiao-Jiang Hao Gang Chen Shu-Zhen Mu Hepatoprotective Constituents of Total Dibenzocyclooctadiene Lignans from <i>Schisandra chinensis</i> Based on the Spectrum-Effect Relationship
description	To scientifically clarify the hepatoprotective constituents of Fructus Schizandrae chinensis, eleven batches samples of total dibenzocyclooctadiene lignans (TDL) from <i>Schisandra chinensis</i> were prepared by using the optimum extraction technique. Characteristic high-performance liquid chromatography (HPLC) chromatograms were obtained through HPLC analysis technology, and the hepatoprotective effects of the eleven batches of TDL were evaluated by MTT assay. Based on the chemical and biological activity results, the spectrum-effect relationship between the characteristic HPLC fingerprints and the hepatoprotective effect of TDL was established using Minitab 16.0 data analysis software. On the basis of the spectrum-effect relationship, thirteen compounds (<b>1</b>–<b>13</b>) were obtained from the TDL by chemical natural product chemical separation and purification technology, and their structures were identified on the basis of the spectral data and the literature. Based on these compounds, thirteen common peaks among the thirty-three chromatographic peaks in the above HPLC fingerprints were identified. Our findings showed that some components, including, schisandrin B (<b>2</b>), schisandrin A (<b>3</b>), and schisandrol B (<b>7</b>) had significant roles in promoting hepatoprotective activity. Preliminary verification of the spectrum-effect relationship of TDL from <i>S. chinensis</i> was carried out, and the results confirmed that the activity of a composite of these three key components in optimal ratios was better than that of any individual compound, which potentially confirmed the reliability of the spectrum-effect relationship and the synergistic effects of traditional Chinese medicine.
format	article
author	Lu-Lu Deng Xu-Dong Xie Jiang Li Dao-Ping Wang Xiao-Jiang Hao Gang Chen Shu-Zhen Mu
author_facet	Lu-Lu Deng Xu-Dong Xie Jiang Li Dao-Ping Wang Xiao-Jiang Hao Gang Chen Shu-Zhen Mu
author_sort	Lu-Lu Deng
title	Hepatoprotective Constituents of Total Dibenzocyclooctadiene Lignans from <i>Schisandra chinensis</i> Based on the Spectrum-Effect Relationship
title_short	Hepatoprotective Constituents of Total Dibenzocyclooctadiene Lignans from <i>Schisandra chinensis</i> Based on the Spectrum-Effect Relationship
title_full	Hepatoprotective Constituents of Total Dibenzocyclooctadiene Lignans from <i>Schisandra chinensis</i> Based on the Spectrum-Effect Relationship
title_fullStr	Hepatoprotective Constituents of Total Dibenzocyclooctadiene Lignans from <i>Schisandra chinensis</i> Based on the Spectrum-Effect Relationship
title_full_unstemmed	Hepatoprotective Constituents of Total Dibenzocyclooctadiene Lignans from <i>Schisandra chinensis</i> Based on the Spectrum-Effect Relationship
title_sort	hepatoprotective constituents of total dibenzocyclooctadiene lignans from <i>schisandra chinensis</i> based on the spectrum-effect relationship
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/de97d641938f479abf1b38d758bbfac5
work_keys_str_mv	AT luludeng hepatoprotectiveconstituentsoftotaldibenzocyclooctadienelignansfromischisandrachinensisibasedonthespectrumeffectrelationship AT xudongxie hepatoprotectiveconstituentsoftotaldibenzocyclooctadienelignansfromischisandrachinensisibasedonthespectrumeffectrelationship AT jiangli hepatoprotectiveconstituentsoftotaldibenzocyclooctadienelignansfromischisandrachinensisibasedonthespectrumeffectrelationship AT daopingwang hepatoprotectiveconstituentsoftotaldibenzocyclooctadienelignansfromischisandrachinensisibasedonthespectrumeffectrelationship AT xiaojianghao hepatoprotectiveconstituentsoftotaldibenzocyclooctadienelignansfromischisandrachinensisibasedonthespectrumeffectrelationship AT gangchen hepatoprotectiveconstituentsoftotaldibenzocyclooctadienelignansfromischisandrachinensisibasedonthespectrumeffectrelationship AT shuzhenmu hepatoprotectiveconstituentsoftotaldibenzocyclooctadienelignansfromischisandrachinensisibasedonthespectrumeffectrelationship
_version_	1718431855058878464

Hepatoprotective Constituents of Total Dibenzocyclooctadiene Lignans from <i>Schisandra chinensis</i> Based on the Spectrum-Effect Relationship

Ejemplares similares