Circulating CitH3 Is a Reliable Diagnostic and Prognostic Biomarker of Septic Patients in Acute Pancreatitis

PurposeAcute pancreatitis (AP) is an inflammatory disease. AP starts with sterile inflammation and is often complicated with critical local or systemic infection or sepsis in severe cases. Septic AP activates peptidyl arginine deiminase (PAD) and citrullinates histone H3 (CitH3), leading to neutroph...

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Autores principales:	Baihong Pan, Yaozhen Li, Yu Liu, Wei Wang, Gengwen Huang, Yang Ouyang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	acute pancreatitis sepsis citrullinated histone H3 peptidyl arginine deiminase diagnosis prognosis Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/dea095e18d1947f5a8853b67ae5cda21
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spelling	oai:doaj.org-article:dea095e18d1947f5a8853b67ae5cda212021-11-17T04:42:29ZCirculating CitH3 Is a Reliable Diagnostic and Prognostic Biomarker of Septic Patients in Acute Pancreatitis1664-322410.3389/fimmu.2021.766391https://doaj.org/article/dea095e18d1947f5a8853b67ae5cda212021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.766391/fullhttps://doaj.org/toc/1664-3224PurposeAcute pancreatitis (AP) is an inflammatory disease. AP starts with sterile inflammation and is often complicated with critical local or systemic infection or sepsis in severe cases. Septic AP activates peptidyl arginine deiminase (PAD) and citrullinates histone H3 (CitH3), leading to neutrophil extracellular trap (NET) formation. Investigating the role of NETs and underlying mechanisms in septic AP may facilitate developing diagnostic and therapeutic approaches. In this study, we sought to identify the expression of CitH3 in septic AP patients and to analyze the correlation of CitH3 concentration with NET components as well as clinical outcomes.MethodsSeventy AP patients with or without sepsis (40 septic cases, 30 nonseptic cases) and 30 healthy volunteers were recruited in this study. Concentration of NET components (CitH3 and double-strain DNA) and key enzymes (PAD2/4) were measured. Clinical and laboratory characteristics of patients were recorded and analyzed.ResultsLevels of CitH3 were elevated significantly in septic AP patients compared with those in nonseptic AP and healthy volunteers. The area under the curve (AUC, 95% confidence interval) for diagnosing septic AP was 0.93 (0.86–1.003), and the cutoff was 43.05 pg/ml. Among septic AP cases (n = 40), the concentration of CitH3 was significantly increased in those who did not survive or were admitted to the intensive care unit, when compared with that in those who survived or did not require intensive care unit. Association analysis revealed that CitH3 concentration was positively correlated with PAD2, PAD4, dsDNA concentration, and Sequential Organ Failure Assessment scores.ConclusionCitH3 concentration increased in septic AP patients and was closely correlated with disease severity and clinical outcomes. CitH3 may potentially be a diagnostic and prognostic biomarker of septic AP.Baihong PanYaozhen LiYu LiuWei WangGengwen HuangYang OuyangFrontiers Media S.A.articleacute pancreatitissepsiscitrullinated histone H3peptidyl arginine deiminasediagnosisprognosisImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	acute pancreatitis sepsis citrullinated histone H3 peptidyl arginine deiminase diagnosis prognosis Immunologic diseases. Allergy RC581-607
spellingShingle	acute pancreatitis sepsis citrullinated histone H3 peptidyl arginine deiminase diagnosis prognosis Immunologic diseases. Allergy RC581-607 Baihong Pan Yaozhen Li Yu Liu Wei Wang Gengwen Huang Yang Ouyang Circulating CitH3 Is a Reliable Diagnostic and Prognostic Biomarker of Septic Patients in Acute Pancreatitis
description	PurposeAcute pancreatitis (AP) is an inflammatory disease. AP starts with sterile inflammation and is often complicated with critical local or systemic infection or sepsis in severe cases. Septic AP activates peptidyl arginine deiminase (PAD) and citrullinates histone H3 (CitH3), leading to neutrophil extracellular trap (NET) formation. Investigating the role of NETs and underlying mechanisms in septic AP may facilitate developing diagnostic and therapeutic approaches. In this study, we sought to identify the expression of CitH3 in septic AP patients and to analyze the correlation of CitH3 concentration with NET components as well as clinical outcomes.MethodsSeventy AP patients with or without sepsis (40 septic cases, 30 nonseptic cases) and 30 healthy volunteers were recruited in this study. Concentration of NET components (CitH3 and double-strain DNA) and key enzymes (PAD2/4) were measured. Clinical and laboratory characteristics of patients were recorded and analyzed.ResultsLevels of CitH3 were elevated significantly in septic AP patients compared with those in nonseptic AP and healthy volunteers. The area under the curve (AUC, 95% confidence interval) for diagnosing septic AP was 0.93 (0.86–1.003), and the cutoff was 43.05 pg/ml. Among septic AP cases (n = 40), the concentration of CitH3 was significantly increased in those who did not survive or were admitted to the intensive care unit, when compared with that in those who survived or did not require intensive care unit. Association analysis revealed that CitH3 concentration was positively correlated with PAD2, PAD4, dsDNA concentration, and Sequential Organ Failure Assessment scores.ConclusionCitH3 concentration increased in septic AP patients and was closely correlated with disease severity and clinical outcomes. CitH3 may potentially be a diagnostic and prognostic biomarker of septic AP.
format	article
author	Baihong Pan Yaozhen Li Yu Liu Wei Wang Gengwen Huang Yang Ouyang
author_facet	Baihong Pan Yaozhen Li Yu Liu Wei Wang Gengwen Huang Yang Ouyang
author_sort	Baihong Pan
title	Circulating CitH3 Is a Reliable Diagnostic and Prognostic Biomarker of Septic Patients in Acute Pancreatitis
title_short	Circulating CitH3 Is a Reliable Diagnostic and Prognostic Biomarker of Septic Patients in Acute Pancreatitis
title_full	Circulating CitH3 Is a Reliable Diagnostic and Prognostic Biomarker of Septic Patients in Acute Pancreatitis
title_fullStr	Circulating CitH3 Is a Reliable Diagnostic and Prognostic Biomarker of Septic Patients in Acute Pancreatitis
title_full_unstemmed	Circulating CitH3 Is a Reliable Diagnostic and Prognostic Biomarker of Septic Patients in Acute Pancreatitis
title_sort	circulating cith3 is a reliable diagnostic and prognostic biomarker of septic patients in acute pancreatitis
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/dea095e18d1947f5a8853b67ae5cda21
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Circulating CitH3 Is a Reliable Diagnostic and Prognostic Biomarker of Septic Patients in Acute Pancreatitis

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