Molecular characteristics of two phenotypically identical species of Asteraceae based on the intergenic spacer trnT(UGU)-trnL(UAA)

Abstract. Susanto AH, Dwiati M, Pratiwi S. 2020. Molecular characteristics of two phenotypically identical species of Asteraceae based on the intergenic spacer trnT(UGU)-trnL(UAA). Biodiversitas 21: 5164-5169. Ogiera (Eleutheranthera ruderalis) and nodeweed (Synedrella nodiflora) are two different w...

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Autores principales:	AGUS HERY SUSANTO, Murni Dwiati, Salabila Pratiwi
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	eleutheranthera ruderalis, genetic differences, igs trnt(ugu)-trnl(uaa), synedrella nodiflora Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/dea19ba110384ad1beb5915a28d4157d
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spelling	oai:doaj.org-article:dea19ba110384ad1beb5915a28d4157d2021-11-22T00:44:11ZMolecular characteristics of two phenotypically identical species of Asteraceae based on the intergenic spacer trnT(UGU)-trnL(UAA)1412-033X2085-472210.13057/biodiv/d211122https://doaj.org/article/dea19ba110384ad1beb5915a28d4157d2020-10-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6258https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Susanto AH, Dwiati M, Pratiwi S. 2020. Molecular characteristics of two phenotypically identical species of Asteraceae based on the intergenic spacer trnT(UGU)-trnL(UAA). Biodiversitas 21: 5164-5169. Ogiera (Eleutheranthera ruderalis) and nodeweed (Synedrella nodiflora) are two different weed species belonging to the family Asteraceae commonly found in tropical regions. At a glance, both species show highly identical morphology, thus leading to difficulty in distinguishing between them. Therefore, molecular data based on particular markers are required. Here, we use an intergenic spacer (IGS) in the cpDNA, i.e., trnT(UGU)-trnL(UAA), as the molecular marker to reveal the difference between the two species. A pair of PCR universal primers, i.e., B48557 as the forward primer and A49291 as the reverse primer, were employed to amplify the marker. Sequence alignment was performed by the use of ClustalW implemented in Bioedit version 7.0.4.1. The results revealed that some differences with respect to both indel and base substitution were observed. Overall, this led to longer IGS trnT(UGU)-trnL(UAA) sequences of E. ruderalis than those of S. nodiflora. Although no direct relationship between the genetic and phenotypic dissimilarities was proven, coincidence seemed likely to exist. This provides molecular evidence that the two phenotypically similar species are genetically different from each other.AGUS HERY SUSANTOMurni DwiatiSalabila PratiwiMBI & UNS Soloarticleeleutheranthera ruderalis, genetic differences, igs trnt(ugu)-trnl(uaa), synedrella nodifloraBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	eleutheranthera ruderalis, genetic differences, igs trnt(ugu)-trnl(uaa), synedrella nodiflora Biology (General) QH301-705.5
spellingShingle	eleutheranthera ruderalis, genetic differences, igs trnt(ugu)-trnl(uaa), synedrella nodiflora Biology (General) QH301-705.5 AGUS HERY SUSANTO Murni Dwiati Salabila Pratiwi Molecular characteristics of two phenotypically identical species of Asteraceae based on the intergenic spacer trnT(UGU)-trnL(UAA)
description	Abstract. Susanto AH, Dwiati M, Pratiwi S. 2020. Molecular characteristics of two phenotypically identical species of Asteraceae based on the intergenic spacer trnT(UGU)-trnL(UAA). Biodiversitas 21: 5164-5169. Ogiera (Eleutheranthera ruderalis) and nodeweed (Synedrella nodiflora) are two different weed species belonging to the family Asteraceae commonly found in tropical regions. At a glance, both species show highly identical morphology, thus leading to difficulty in distinguishing between them. Therefore, molecular data based on particular markers are required. Here, we use an intergenic spacer (IGS) in the cpDNA, i.e., trnT(UGU)-trnL(UAA), as the molecular marker to reveal the difference between the two species. A pair of PCR universal primers, i.e., B48557 as the forward primer and A49291 as the reverse primer, were employed to amplify the marker. Sequence alignment was performed by the use of ClustalW implemented in Bioedit version 7.0.4.1. The results revealed that some differences with respect to both indel and base substitution were observed. Overall, this led to longer IGS trnT(UGU)-trnL(UAA) sequences of E. ruderalis than those of S. nodiflora. Although no direct relationship between the genetic and phenotypic dissimilarities was proven, coincidence seemed likely to exist. This provides molecular evidence that the two phenotypically similar species are genetically different from each other.
format	article
author	AGUS HERY SUSANTO Murni Dwiati Salabila Pratiwi
author_facet	AGUS HERY SUSANTO Murni Dwiati Salabila Pratiwi
author_sort	AGUS HERY SUSANTO
title	Molecular characteristics of two phenotypically identical species of Asteraceae based on the intergenic spacer trnT(UGU)-trnL(UAA)
title_short	Molecular characteristics of two phenotypically identical species of Asteraceae based on the intergenic spacer trnT(UGU)-trnL(UAA)
title_full	Molecular characteristics of two phenotypically identical species of Asteraceae based on the intergenic spacer trnT(UGU)-trnL(UAA)
title_fullStr	Molecular characteristics of two phenotypically identical species of Asteraceae based on the intergenic spacer trnT(UGU)-trnL(UAA)
title_full_unstemmed	Molecular characteristics of two phenotypically identical species of Asteraceae based on the intergenic spacer trnT(UGU)-trnL(UAA)
title_sort	molecular characteristics of two phenotypically identical species of asteraceae based on the intergenic spacer trnt(ugu)-trnl(uaa)
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/dea19ba110384ad1beb5915a28d4157d
work_keys_str_mv	AT agusherysusanto molecularcharacteristicsoftwophenotypicallyidenticalspeciesofasteraceaebasedontheintergenicspacertrntugutrnluaa AT murnidwiati molecularcharacteristicsoftwophenotypicallyidenticalspeciesofasteraceaebasedontheintergenicspacertrntugutrnluaa AT salabilapratiwi molecularcharacteristicsoftwophenotypicallyidenticalspeciesofasteraceaebasedontheintergenicspacertrntugutrnluaa
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Molecular characteristics of two phenotypically identical species of Asteraceae based on the intergenic spacer trnT(UGU)-trnL(UAA)

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