Surface ligand controls silver ion release of nanosilver and its antibacterial activity against Escherichia coli

Yan-Min Long,1,2 Li-Gang Hu,1,3 Xue-Ting Yan,1,3 Xing-Chen Zhao,1,3 Qun-Fang Zhou,1,3 Yong Cai,2,4 Gui-Bin Jiang1,3 1State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Beijing, China; 2Institute of Environment and Health, Jianghan Univ...

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Autores principales:	Long Y, Hu L, Yan X, Zhao X, Zhou Q, Cai Y, Jiang G
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	silver nanoparticle surface chemistry silver ion release trojan horse mechanism respiratory chain oxidative stress Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/dea67b91fef64db98f28ddd5bc62cc89
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spelling	oai:doaj.org-article:dea67b91fef64db98f28ddd5bc62cc892021-12-02T07:22:59ZSurface ligand controls silver ion release of nanosilver and its antibacterial activity against Escherichia coli1178-2013https://doaj.org/article/dea67b91fef64db98f28ddd5bc62cc892017-04-01T00:00:00Zhttps://www.dovepress.com/surface-ligand-controls-silver-ion-release-of-nanosilver-and-its-antib-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yan-Min Long,1,2 Li-Gang Hu,1,3 Xue-Ting Yan,1,3 Xing-Chen Zhao,1,3 Qun-Fang Zhou,1,3 Yong Cai,2,4 Gui-Bin Jiang1,3 1State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Beijing, China; 2Institute of Environment and Health, Jianghan University, Wuhan, Hubei, China; 3College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, China; 4Department of Chemistry and Biochemistry, Southeast Environmental Research Center, Florida International University, Miami, FL, USA Abstract: Understanding the mechanism of nanosilver-dependent antibacterial activity against microorganisms helps optimize the design and usage of the related nanomaterials. In this study, we prepared four kinds of 10 nm-sized silver nanoparticles (AgNPs) with dictated surface chemistry by capping different ligands, including citrate, mercaptopropionic acid, mercaptohexanoic acid, and mercaptopropionic sulfonic acid. Their surface-dependent chemistry and antibacterial activities were investigated. Owing to the weak bond to surface Ag, short carbon chain, and low silver ion attraction, citrate-coated AgNPs caused the highest silver ion release and the strongest antibacterial activity against Escherichia coli, when compared to the other tested AgNPs. The study on the underlying antibacterial mechanisms indicated that cellular membrane uptake of Ag, NAD+/NADH ratio increase, and intracellular reactive oxygen species (ROS) generation were significantly induced in both AgNP and silver ion exposure groups. The released silver ions from AgNPs inside cells through a Trojan-horse-type mechanism were suggested to interact with respiratory chain proteins on the membrane, interrupt intracellular O2 reduction, and induce ROS production. The further oxidative damages of lipid peroxidation and membrane breakdown caused the lethal effect on E. coli. Altogether, this study demonstrated that AgNPs exerted antibacterial activity through the release of silver ions and the subsequent induction of intracellular ROS generation by interacting with the cell membrane. The findings are helpful in guiding the controllable synthesis through the regulation of surface coating for medical care purpose. Keywords: silver nanoparticles, surface chemistry, silver ion release, Trojan-horse-type mechanism, respiratory chain, oxidative stressLong YHu LYan XZhao XZhou QCai YJiang GDove Medical Pressarticlesilver nanoparticlesurface chemistrysilver ion releasetrojan horse mechanismrespiratory chainoxidative stressMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 3193-3206 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	silver nanoparticle surface chemistry silver ion release trojan horse mechanism respiratory chain oxidative stress Medicine (General) R5-920
spellingShingle	silver nanoparticle surface chemistry silver ion release trojan horse mechanism respiratory chain oxidative stress Medicine (General) R5-920 Long Y Hu L Yan X Zhao X Zhou Q Cai Y Jiang G Surface ligand controls silver ion release of nanosilver and its antibacterial activity against Escherichia coli
description	Yan-Min Long,1,2 Li-Gang Hu,1,3 Xue-Ting Yan,1,3 Xing-Chen Zhao,1,3 Qun-Fang Zhou,1,3 Yong Cai,2,4 Gui-Bin Jiang1,3 1State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Beijing, China; 2Institute of Environment and Health, Jianghan University, Wuhan, Hubei, China; 3College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, China; 4Department of Chemistry and Biochemistry, Southeast Environmental Research Center, Florida International University, Miami, FL, USA Abstract: Understanding the mechanism of nanosilver-dependent antibacterial activity against microorganisms helps optimize the design and usage of the related nanomaterials. In this study, we prepared four kinds of 10 nm-sized silver nanoparticles (AgNPs) with dictated surface chemistry by capping different ligands, including citrate, mercaptopropionic acid, mercaptohexanoic acid, and mercaptopropionic sulfonic acid. Their surface-dependent chemistry and antibacterial activities were investigated. Owing to the weak bond to surface Ag, short carbon chain, and low silver ion attraction, citrate-coated AgNPs caused the highest silver ion release and the strongest antibacterial activity against Escherichia coli, when compared to the other tested AgNPs. The study on the underlying antibacterial mechanisms indicated that cellular membrane uptake of Ag, NAD+/NADH ratio increase, and intracellular reactive oxygen species (ROS) generation were significantly induced in both AgNP and silver ion exposure groups. The released silver ions from AgNPs inside cells through a Trojan-horse-type mechanism were suggested to interact with respiratory chain proteins on the membrane, interrupt intracellular O2 reduction, and induce ROS production. The further oxidative damages of lipid peroxidation and membrane breakdown caused the lethal effect on E. coli. Altogether, this study demonstrated that AgNPs exerted antibacterial activity through the release of silver ions and the subsequent induction of intracellular ROS generation by interacting with the cell membrane. The findings are helpful in guiding the controllable synthesis through the regulation of surface coating for medical care purpose. Keywords: silver nanoparticles, surface chemistry, silver ion release, Trojan-horse-type mechanism, respiratory chain, oxidative stress
format	article
author	Long Y Hu L Yan X Zhao X Zhou Q Cai Y Jiang G
author_facet	Long Y Hu L Yan X Zhao X Zhou Q Cai Y Jiang G
author_sort	Long Y
title	Surface ligand controls silver ion release of nanosilver and its antibacterial activity against Escherichia coli
title_short	Surface ligand controls silver ion release of nanosilver and its antibacterial activity against Escherichia coli
title_full	Surface ligand controls silver ion release of nanosilver and its antibacterial activity against Escherichia coli
title_fullStr	Surface ligand controls silver ion release of nanosilver and its antibacterial activity against Escherichia coli
title_full_unstemmed	Surface ligand controls silver ion release of nanosilver and its antibacterial activity against Escherichia coli
title_sort	surface ligand controls silver ion release of nanosilver and its antibacterial activity against escherichia coli
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/dea67b91fef64db98f28ddd5bc62cc89
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Surface ligand controls silver ion release of nanosilver and its antibacterial activity against Escherichia coli

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