Screening of anti-microbial, anti-biofilm activity, and cytotoxicity analysis of a designed polyherbal formulation against shigellosis

Background: Shigellosis is an infectious intestinal disease common in rural communities. In developing countries, shigellosis is caused predominantly by Shigella flexneri and has been determined as a major cause of morbidity and mortality. Objective: The study investigates the anti-biofilm, anti-mic...

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Autores principales:	Devendra Singh, Deepmala Sharma, Vishnu Agarwal
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Shigellosis Shigella flexneri Formulation Antibiofilm Anti-quorum sensing Miscellaneous systems and treatments RZ409.7-999
Acceso en línea:	https://doaj.org/article/dea74a23706d4699bf7623e953a4f2f9
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spelling	oai:doaj.org-article:dea74a23706d4699bf7623e953a4f2f92021-12-02T04:59:19ZScreening of anti-microbial, anti-biofilm activity, and cytotoxicity analysis of a designed polyherbal formulation against shigellosis0975-947610.1016/j.jaim.2021.06.007https://doaj.org/article/dea74a23706d4699bf7623e953a4f2f92021-10-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0975947621001157https://doaj.org/toc/0975-9476Background: Shigellosis is an infectious intestinal disease common in rural communities. In developing countries, shigellosis is caused predominantly by Shigella flexneri and has been determined as a major cause of morbidity and mortality. Objective: The study investigates the anti-biofilm, anti-microbial, and anti-shigellosis activity of a designed formulation. Materials and methods: The potential of the formulation against S. flexneri (MTCC 1457) was investigated using a well-diffusion assay. Further, the effect of the designed formulation on bacterial growth and biofilm formation was analyzed by the spectrophotometry method. Anti-quorum sensing activity was analyzed using Chromobacterium violaceum CV026, a bacterial strain. Finally, the cytotoxicity of the formulation was examined by using cell line and brine shrimp lethality assay. Results: The MIC value of the aqueous extract of the formulation was 2.4 mg ml−1 and an inhibitory zone of 23 mm was observed. On the other side, the formulation significantly inhibited the bacterial growth, biofilm formation (23.78%), violacein inhibition (27.68%) at 0.6 mg ml−1 concentration (did not significantly affect the growth curves) and was found non-toxic in cell assay and brine shrimp lethality assay. Conclusion: According to the result obtained, the designed formulation was found effective and non-toxic, so it can be used to treat shigellosis and Shigella-related infections.Devendra SinghDeepmala SharmaVishnu AgarwalElsevierarticleShigellosisShigella flexneriFormulationAntibiofilmAnti-quorum sensingMiscellaneous systems and treatmentsRZ409.7-999ENJournal of Ayurveda and Integrative Medicine, Vol 12, Iss 4, Pp 601-606 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Shigellosis Shigella flexneri Formulation Antibiofilm Anti-quorum sensing Miscellaneous systems and treatments RZ409.7-999
spellingShingle	Shigellosis Shigella flexneri Formulation Antibiofilm Anti-quorum sensing Miscellaneous systems and treatments RZ409.7-999 Devendra Singh Deepmala Sharma Vishnu Agarwal Screening of anti-microbial, anti-biofilm activity, and cytotoxicity analysis of a designed polyherbal formulation against shigellosis
description	Background: Shigellosis is an infectious intestinal disease common in rural communities. In developing countries, shigellosis is caused predominantly by Shigella flexneri and has been determined as a major cause of morbidity and mortality. Objective: The study investigates the anti-biofilm, anti-microbial, and anti-shigellosis activity of a designed formulation. Materials and methods: The potential of the formulation against S. flexneri (MTCC 1457) was investigated using a well-diffusion assay. Further, the effect of the designed formulation on bacterial growth and biofilm formation was analyzed by the spectrophotometry method. Anti-quorum sensing activity was analyzed using Chromobacterium violaceum CV026, a bacterial strain. Finally, the cytotoxicity of the formulation was examined by using cell line and brine shrimp lethality assay. Results: The MIC value of the aqueous extract of the formulation was 2.4 mg ml−1 and an inhibitory zone of 23 mm was observed. On the other side, the formulation significantly inhibited the bacterial growth, biofilm formation (23.78%), violacein inhibition (27.68%) at 0.6 mg ml−1 concentration (did not significantly affect the growth curves) and was found non-toxic in cell assay and brine shrimp lethality assay. Conclusion: According to the result obtained, the designed formulation was found effective and non-toxic, so it can be used to treat shigellosis and Shigella-related infections.
format	article
author	Devendra Singh Deepmala Sharma Vishnu Agarwal
author_facet	Devendra Singh Deepmala Sharma Vishnu Agarwal
author_sort	Devendra Singh
title	Screening of anti-microbial, anti-biofilm activity, and cytotoxicity analysis of a designed polyherbal formulation against shigellosis
title_short	Screening of anti-microbial, anti-biofilm activity, and cytotoxicity analysis of a designed polyherbal formulation against shigellosis
title_full	Screening of anti-microbial, anti-biofilm activity, and cytotoxicity analysis of a designed polyherbal formulation against shigellosis
title_fullStr	Screening of anti-microbial, anti-biofilm activity, and cytotoxicity analysis of a designed polyherbal formulation against shigellosis
title_full_unstemmed	Screening of anti-microbial, anti-biofilm activity, and cytotoxicity analysis of a designed polyherbal formulation against shigellosis
title_sort	screening of anti-microbial, anti-biofilm activity, and cytotoxicity analysis of a designed polyherbal formulation against shigellosis
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/dea74a23706d4699bf7623e953a4f2f9
work_keys_str_mv	AT devendrasingh screeningofantimicrobialantibiofilmactivityandcytotoxicityanalysisofadesignedpolyherbalformulationagainstshigellosis AT deepmalasharma screeningofantimicrobialantibiofilmactivityandcytotoxicityanalysisofadesignedpolyherbalformulationagainstshigellosis AT vishnuagarwal screeningofantimicrobialantibiofilmactivityandcytotoxicityanalysisofadesignedpolyherbalformulationagainstshigellosis
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Screening of anti-microbial, anti-biofilm activity, and cytotoxicity analysis of a designed polyherbal formulation against shigellosis

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