Functional roles of multiple Ton complex genes in a Sphingobium degrader of lignin-derived aromatic compounds

Abstract TonB-dependent transporters (TBDTs) mediate outer membrane transport of nutrients using the energy derived from proton motive force transmitted from the TonB–ExbB–ExbD complex localized in the inner membrane. Recently, we discovered ddvT encoding a TBDT responsible for the uptake of a 5,5-t...

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Autores principales:	Masaya Fujita, Shodai Yano, Koki Shibata, Mizuki Kondo, Shojiro Hishiyama, Naofumi Kamimura, Eiji Masai
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dea7b7d7dbb74d5e829bfee7e0877de5
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spelling	oai:doaj.org-article:dea7b7d7dbb74d5e829bfee7e0877de52021-11-21T12:16:15ZFunctional roles of multiple Ton complex genes in a Sphingobium degrader of lignin-derived aromatic compounds10.1038/s41598-021-01756-82045-2322https://doaj.org/article/dea7b7d7dbb74d5e829bfee7e0877de52021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01756-8https://doaj.org/toc/2045-2322Abstract TonB-dependent transporters (TBDTs) mediate outer membrane transport of nutrients using the energy derived from proton motive force transmitted from the TonB–ExbB–ExbD complex localized in the inner membrane. Recently, we discovered ddvT encoding a TBDT responsible for the uptake of a 5,5-type lignin-derived dimer in Sphingobium sp. strain SYK-6. Furthermore, overexpression of ddvT in an SYK-6-derivative strain enhanced its uptake capacity, improving the rate of platform chemical production. Thus, understanding the uptake system of lignin-derived aromatics is fundamental for microbial conversion-based lignin valorization. Here we examined whether multiple tonB-, exbB-, and exbD-like genes in SYK-6 contribute to the outer membrane transport of lignin-derived aromatics. The disruption of tonB2–6 and exbB3 did not reduce the capacity of SYK-6 to convert or grow on lignin-derived aromatics. In contrast, the introduction of the tonB1–exbB1–exbD1–exbD2 operon genes into SYK-6, which could not be disrupted, promoted the conversion of β-O-4-, β-5-, β-1-, β-β-, and 5,5-type dimers and monomers, such as ferulate, vanillate, syringate, and protocatechuate. These results suggest that TonB-dependent uptake involving the tonB1 operon genes is responsible for the outer membrane transport of the above aromatics. Additionally, exbB2/tolQ and exbD3/tolR were suggested to constitute the Tol-Pal system that maintains the outer membrane integrity.Masaya FujitaShodai YanoKoki ShibataMizuki KondoShojiro HishiyamaNaofumi KamimuraEiji MasaiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Masaya Fujita Shodai Yano Koki Shibata Mizuki Kondo Shojiro Hishiyama Naofumi Kamimura Eiji Masai Functional roles of multiple Ton complex genes in a Sphingobium degrader of lignin-derived aromatic compounds
description	Abstract TonB-dependent transporters (TBDTs) mediate outer membrane transport of nutrients using the energy derived from proton motive force transmitted from the TonB–ExbB–ExbD complex localized in the inner membrane. Recently, we discovered ddvT encoding a TBDT responsible for the uptake of a 5,5-type lignin-derived dimer in Sphingobium sp. strain SYK-6. Furthermore, overexpression of ddvT in an SYK-6-derivative strain enhanced its uptake capacity, improving the rate of platform chemical production. Thus, understanding the uptake system of lignin-derived aromatics is fundamental for microbial conversion-based lignin valorization. Here we examined whether multiple tonB-, exbB-, and exbD-like genes in SYK-6 contribute to the outer membrane transport of lignin-derived aromatics. The disruption of tonB2–6 and exbB3 did not reduce the capacity of SYK-6 to convert or grow on lignin-derived aromatics. In contrast, the introduction of the tonB1–exbB1–exbD1–exbD2 operon genes into SYK-6, which could not be disrupted, promoted the conversion of β-O-4-, β-5-, β-1-, β-β-, and 5,5-type dimers and monomers, such as ferulate, vanillate, syringate, and protocatechuate. These results suggest that TonB-dependent uptake involving the tonB1 operon genes is responsible for the outer membrane transport of the above aromatics. Additionally, exbB2/tolQ and exbD3/tolR were suggested to constitute the Tol-Pal system that maintains the outer membrane integrity.
format	article
author	Masaya Fujita Shodai Yano Koki Shibata Mizuki Kondo Shojiro Hishiyama Naofumi Kamimura Eiji Masai
author_facet	Masaya Fujita Shodai Yano Koki Shibata Mizuki Kondo Shojiro Hishiyama Naofumi Kamimura Eiji Masai
author_sort	Masaya Fujita
title	Functional roles of multiple Ton complex genes in a Sphingobium degrader of lignin-derived aromatic compounds
title_short	Functional roles of multiple Ton complex genes in a Sphingobium degrader of lignin-derived aromatic compounds
title_full	Functional roles of multiple Ton complex genes in a Sphingobium degrader of lignin-derived aromatic compounds
title_fullStr	Functional roles of multiple Ton complex genes in a Sphingobium degrader of lignin-derived aromatic compounds
title_full_unstemmed	Functional roles of multiple Ton complex genes in a Sphingobium degrader of lignin-derived aromatic compounds
title_sort	functional roles of multiple ton complex genes in a sphingobium degrader of lignin-derived aromatic compounds
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/dea7b7d7dbb74d5e829bfee7e0877de5
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Functional roles of multiple Ton complex genes in a Sphingobium degrader of lignin-derived aromatic compounds

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