Laccase Activity in Fungus Cryphonectria parasitica Is Affected by Growth Conditions and Fungal–Viral Genotypic Interactions

Laccase activity reduction in the chestnut blight fungus Cryphonectria parasitica usually accompanies the hypovirulence caused by the infection of fungus with Cryphonectria hypovirus 1 (CHV1). However, the different methods utilized for assessing this phenomenon...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lucija Nuskern, Mirta Tkalec, Bruno Srezović, Marin Ježić, Martina Gačar, Mirna Ćurković-Perica
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	chestnut blight <i>Cryphonectria hypovirus 1</i> virus effect Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/dea840ab6b5649a0b09e5d27cc2c4023
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:dea840ab6b5649a0b09e5d27cc2c4023
record_format	dspace
spelling	oai:doaj.org-article:dea840ab6b5649a0b09e5d27cc2c40232021-11-25T18:06:11ZLaccase Activity in Fungus <i>Cryphonectria parasitica</i> Is Affected by Growth Conditions and Fungal–Viral Genotypic Interactions10.3390/jof71109582309-608Xhttps://doaj.org/article/dea840ab6b5649a0b09e5d27cc2c40232021-11-01T00:00:00Zhttps://www.mdpi.com/2309-608X/7/11/958https://doaj.org/toc/2309-608XLaccase activity reduction in the chestnut blight fungus <i>Cryphonectria parasitica</i> usually accompanies the hypovirulence caused by the infection of fungus with <i>Cryphonectria hypovirus 1</i> (CHV1). However, the different methods utilized for assessing this phenomenon has produced varied and often conflicting results. Furthermore, the majority of experimental setups included only one prototypic system, further confounding the results. Considering the diversity of fungal isolates, viral strains, and variability of their effects on the phytopathogenic process observed in nature, our goal was to ascertain if laccase activity variability is affected by (1) different <i>C. parasitica</i> isolates infected with several CHV1 strains, and (2) growth conditions. We have demonstrated that some CHV1 strains, contrary to previous assumptions, increase the activity of <i>C. parasitica</i> laccases. The specific fungal isolates used in the experiments and culture conditions also affected the results. Furthermore, we showed that two commonly used laccase substrates, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) and 2,4-dimethoxyphenol, cannot be used interchangeably in <i>C. parasitica</i> laccase activity measurements. Our results illustrate the importance of conducting this type of study in experimental systems and culture conditions that resemble natural conditions as much as possible to be able to infer the most relevant conclusions applicable to natural populations.Lucija NuskernMirta TkalecBruno SrezovićMarin JežićMartina GačarMirna Ćurković-PericaMDPI AGarticlechestnut blight<i>Cryphonectria hypovirus 1</i>virus effectBiology (General)QH301-705.5ENJournal of Fungi, Vol 7, Iss 958, p 958 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chestnut blight <i>Cryphonectria hypovirus 1</i> virus effect Biology (General) QH301-705.5
spellingShingle	chestnut blight <i>Cryphonectria hypovirus 1</i> virus effect Biology (General) QH301-705.5 Lucija Nuskern Mirta Tkalec Bruno Srezović Marin Ježić Martina Gačar Mirna Ćurković-Perica Laccase Activity in Fungus <i>Cryphonectria parasitica</i> Is Affected by Growth Conditions and Fungal–Viral Genotypic Interactions
description	Laccase activity reduction in the chestnut blight fungus <i>Cryphonectria parasitica</i> usually accompanies the hypovirulence caused by the infection of fungus with <i>Cryphonectria hypovirus 1</i> (CHV1). However, the different methods utilized for assessing this phenomenon has produced varied and often conflicting results. Furthermore, the majority of experimental setups included only one prototypic system, further confounding the results. Considering the diversity of fungal isolates, viral strains, and variability of their effects on the phytopathogenic process observed in nature, our goal was to ascertain if laccase activity variability is affected by (1) different <i>C. parasitica</i> isolates infected with several CHV1 strains, and (2) growth conditions. We have demonstrated that some CHV1 strains, contrary to previous assumptions, increase the activity of <i>C. parasitica</i> laccases. The specific fungal isolates used in the experiments and culture conditions also affected the results. Furthermore, we showed that two commonly used laccase substrates, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) and 2,4-dimethoxyphenol, cannot be used interchangeably in <i>C. parasitica</i> laccase activity measurements. Our results illustrate the importance of conducting this type of study in experimental systems and culture conditions that resemble natural conditions as much as possible to be able to infer the most relevant conclusions applicable to natural populations.
format	article
author	Lucija Nuskern Mirta Tkalec Bruno Srezović Marin Ježić Martina Gačar Mirna Ćurković-Perica
author_facet	Lucija Nuskern Mirta Tkalec Bruno Srezović Marin Ježić Martina Gačar Mirna Ćurković-Perica
author_sort	Lucija Nuskern
title	Laccase Activity in Fungus <i>Cryphonectria parasitica</i> Is Affected by Growth Conditions and Fungal–Viral Genotypic Interactions
title_short	Laccase Activity in Fungus <i>Cryphonectria parasitica</i> Is Affected by Growth Conditions and Fungal–Viral Genotypic Interactions
title_full	Laccase Activity in Fungus <i>Cryphonectria parasitica</i> Is Affected by Growth Conditions and Fungal–Viral Genotypic Interactions
title_fullStr	Laccase Activity in Fungus <i>Cryphonectria parasitica</i> Is Affected by Growth Conditions and Fungal–Viral Genotypic Interactions
title_full_unstemmed	Laccase Activity in Fungus <i>Cryphonectria parasitica</i> Is Affected by Growth Conditions and Fungal–Viral Genotypic Interactions
title_sort	laccase activity in fungus <i>cryphonectria parasitica</i> is affected by growth conditions and fungal–viral genotypic interactions
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/dea840ab6b5649a0b09e5d27cc2c4023
work_keys_str_mv	AT lucijanuskern laccaseactivityinfungusicryphonectriaparasiticaiisaffectedbygrowthconditionsandfungalviralgenotypicinteractions AT mirtatkalec laccaseactivityinfungusicryphonectriaparasiticaiisaffectedbygrowthconditionsandfungalviralgenotypicinteractions AT brunosrezovic laccaseactivityinfungusicryphonectriaparasiticaiisaffectedbygrowthconditionsandfungalviralgenotypicinteractions AT marinjezic laccaseactivityinfungusicryphonectriaparasiticaiisaffectedbygrowthconditionsandfungalviralgenotypicinteractions AT martinagacar laccaseactivityinfungusicryphonectriaparasiticaiisaffectedbygrowthconditionsandfungalviralgenotypicinteractions AT mirnacurkovicperica laccaseactivityinfungusicryphonectriaparasiticaiisaffectedbygrowthconditionsandfungalviralgenotypicinteractions
_version_	1718411650651914240

Laccase Activity in Fungus <i>Cryphonectria parasitica</i> Is Affected by Growth Conditions and Fungal–Viral Genotypic Interactions

Ejemplares similares