Nicotine induced ototoxicity in rat cochlear organotypic cultures

Epidemiological evidence has shown that smoking is associated with an increased risk of hearing loss. However, the underlying mechanisms regarding the impact of nicotine on the cochlea remain unclear. This study aimed to investigate the cytotoxic effects of nicotine on cochlear cells using cultured...

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Autores principales:	Zhao Yi, Liang Yue, Pan Chunchen, Tang Xiaomin, Sun Yuxuan, Xu Chenyu, Sun Jiaqiang, Sun Jingwu
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	nicotine cochlea hair cells ototoxicity organotypic culture Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/deaa79bff1fc490dabd514771b3d9652
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spelling	oai:doaj.org-article:deaa79bff1fc490dabd514771b3d96522021-12-05T14:11:05ZNicotine induced ototoxicity in rat cochlear organotypic cultures2081-693610.1515/tnsci-2020-0191https://doaj.org/article/deaa79bff1fc490dabd514771b3d96522021-10-01T00:00:00Zhttps://doi.org/10.1515/tnsci-2020-0191https://doaj.org/toc/2081-6936Epidemiological evidence has shown that smoking is associated with an increased risk of hearing loss. However, the underlying mechanisms regarding the impact of nicotine on the cochlea remain unclear. This study aimed to investigate the cytotoxic effects of nicotine on cochlear cells using cultured cochlear basilar membranes. Cochlear basilar membranes were isolated from newborn rats, cultured, and treated with 1–100 ng/mL nicotine for 48 h. Cuticular plates and stereocilia bundle staining were used to evaluate hair cell (HC) loss. Spiral ganglion neuron and acoustic nerve fiber staining were assessed to evaluate cochlear neural injury. Scanning electron microscopy and transmission electron microscopy imaging were employed to examine cochlear ultrastructural changes. Our results showed that compared to spiral ganglia and nerve fibers, HCs are more susceptible to nicotine-induced toxicity. HC loss was more severe in the basal turn than in the middle and apical turns, while nerve fibers and spiral ganglion cells were morphologically maintained. Ultrastructural changes revealed disordered and damaged stereocilia, swelling and decreased mitochondrial density, swelling, and degranulation of the endoplasmic reticulum. Our results suggest that nicotine causes HCs’ degeneration and loss and may have implications for smoking-related hearing loss.Zhao YiLiang YuePan ChunchenTang XiaominSun YuxuanXu ChenyuSun JiaqiangSun JingwuDe Gruyterarticlenicotinecochleahair cellsototoxicityorganotypic cultureNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENTranslational Neuroscience, Vol 12, Iss 1, Pp 407-414 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	nicotine cochlea hair cells ototoxicity organotypic culture Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	nicotine cochlea hair cells ototoxicity organotypic culture Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Zhao Yi Liang Yue Pan Chunchen Tang Xiaomin Sun Yuxuan Xu Chenyu Sun Jiaqiang Sun Jingwu Nicotine induced ototoxicity in rat cochlear organotypic cultures
description	Epidemiological evidence has shown that smoking is associated with an increased risk of hearing loss. However, the underlying mechanisms regarding the impact of nicotine on the cochlea remain unclear. This study aimed to investigate the cytotoxic effects of nicotine on cochlear cells using cultured cochlear basilar membranes. Cochlear basilar membranes were isolated from newborn rats, cultured, and treated with 1–100 ng/mL nicotine for 48 h. Cuticular plates and stereocilia bundle staining were used to evaluate hair cell (HC) loss. Spiral ganglion neuron and acoustic nerve fiber staining were assessed to evaluate cochlear neural injury. Scanning electron microscopy and transmission electron microscopy imaging were employed to examine cochlear ultrastructural changes. Our results showed that compared to spiral ganglia and nerve fibers, HCs are more susceptible to nicotine-induced toxicity. HC loss was more severe in the basal turn than in the middle and apical turns, while nerve fibers and spiral ganglion cells were morphologically maintained. Ultrastructural changes revealed disordered and damaged stereocilia, swelling and decreased mitochondrial density, swelling, and degranulation of the endoplasmic reticulum. Our results suggest that nicotine causes HCs’ degeneration and loss and may have implications for smoking-related hearing loss.
format	article
author	Zhao Yi Liang Yue Pan Chunchen Tang Xiaomin Sun Yuxuan Xu Chenyu Sun Jiaqiang Sun Jingwu
author_facet	Zhao Yi Liang Yue Pan Chunchen Tang Xiaomin Sun Yuxuan Xu Chenyu Sun Jiaqiang Sun Jingwu
author_sort	Zhao Yi
title	Nicotine induced ototoxicity in rat cochlear organotypic cultures
title_short	Nicotine induced ototoxicity in rat cochlear organotypic cultures
title_full	Nicotine induced ototoxicity in rat cochlear organotypic cultures
title_fullStr	Nicotine induced ototoxicity in rat cochlear organotypic cultures
title_full_unstemmed	Nicotine induced ototoxicity in rat cochlear organotypic cultures
title_sort	nicotine induced ototoxicity in rat cochlear organotypic cultures
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/deaa79bff1fc490dabd514771b3d9652
work_keys_str_mv	AT zhaoyi nicotineinducedototoxicityinratcochlearorganotypiccultures AT liangyue nicotineinducedototoxicityinratcochlearorganotypiccultures AT panchunchen nicotineinducedototoxicityinratcochlearorganotypiccultures AT tangxiaomin nicotineinducedototoxicityinratcochlearorganotypiccultures AT sunyuxuan nicotineinducedototoxicityinratcochlearorganotypiccultures AT xuchenyu nicotineinducedototoxicityinratcochlearorganotypiccultures AT sunjiaqiang nicotineinducedototoxicityinratcochlearorganotypiccultures AT sunjingwu nicotineinducedototoxicityinratcochlearorganotypiccultures
_version_	1718371448536432640

Nicotine induced ototoxicity in rat cochlear organotypic cultures

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