Resveratrol Hinders Postovulatory Aging by Modulating Oxidative Stress in Porcine Oocytes

Postovulatory aging of the mammalian oocytes causes deterioration of oocytes through several factors including oxidative stress. Keeping that in mind, we aimed to investigate the potential of a well-known antioxidant, resveratrol (RV), to evaluate the adverse effects of postovulatory aging in porcin...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Benazir Abbasi, Yan Dong, Rong Rui
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	resveratrol postovulatory aging oocyte quality oxidative stress reactive oxygen species Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/deac7d5ff94446879e8d31f6b686b6c9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:deac7d5ff94446879e8d31f6b686b6c9
record_format	dspace
spelling	oai:doaj.org-article:deac7d5ff94446879e8d31f6b686b6c92021-11-11T18:22:43ZResveratrol Hinders Postovulatory Aging by Modulating Oxidative Stress in Porcine Oocytes10.3390/molecules262163461420-3049https://doaj.org/article/deac7d5ff94446879e8d31f6b686b6c92021-10-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6346https://doaj.org/toc/1420-3049Postovulatory aging of the mammalian oocytes causes deterioration of oocytes through several factors including oxidative stress. Keeping that in mind, we aimed to investigate the potential of a well-known antioxidant, resveratrol (RV), to evaluate the adverse effects of postovulatory aging in porcine oocytes. After in vitro maturation (IVM), a group of (25–30) oocytes (in three replicates) were exposed to 0, 1, 2, and 4 μmol/L of RV, respectively. The results revealed that the first polar body (PB1) extrusion rate of the oocytes significantly increased when the RV concentration reached up to 2 μmol/L (<i>p</i> < 0.05). Considering optimum RV concentration of 2 μmol/L, the potential of RV was evaluated in oocytes aged for 24 and 48 h. We used fluorescence microscopy to detect the relative level of reactive oxygen species (ROS), while GHS contents were measured through the enzymatic method. Our results revealed that aged groups (24 h and 48 h) treated with RV (2 μmol/L) showed higher (<i>p</i> < 0.05) ROS fluorescence intensity than the control group, but lower (<i>p</i> < 0.05) than untreated aged groups. The GSH content in untreated aged groups (24 h and 48 h) was lower (<i>p</i> < 0.05) than RV-treated groups, but both groups showed higher levels than the control. Similarly, the relative expression of the genes involved in antioxidant activity (<i>CAT</i>, <i>GPXGSH-Px</i>, and <i>SOD1)</i> in RV-treated groups was lower (<i>p</i> < 0.05) as compared to the control group but higher than that of untreated aged groups. Moreover, the relative mRNA expression of caspase-3 and Bax in RV-treated groups was higher (<i>p</i> < 0.05) than the control group but lower than untreated groups. Furthermore, the expression of <i>Bcl-2</i> in the RV-treated group was significantly lower than control but higher than untreated aged groups. Taken together, our findings revealed that the RV can increase the expression of antioxidant genes by decreasing the level of ROS, and its potent antiapoptotic effects resisted against the decline in mitochondrial membrane potential in aged oocytes.Benazir AbbasiYan DongRong RuiMDPI AGarticleresveratrolpostovulatory agingoocyte qualityoxidative stressreactive oxygen speciesOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6346, p 6346 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	resveratrol postovulatory aging oocyte quality oxidative stress reactive oxygen species Organic chemistry QD241-441
spellingShingle	resveratrol postovulatory aging oocyte quality oxidative stress reactive oxygen species Organic chemistry QD241-441 Benazir Abbasi Yan Dong Rong Rui Resveratrol Hinders Postovulatory Aging by Modulating Oxidative Stress in Porcine Oocytes
description	Postovulatory aging of the mammalian oocytes causes deterioration of oocytes through several factors including oxidative stress. Keeping that in mind, we aimed to investigate the potential of a well-known antioxidant, resveratrol (RV), to evaluate the adverse effects of postovulatory aging in porcine oocytes. After in vitro maturation (IVM), a group of (25–30) oocytes (in three replicates) were exposed to 0, 1, 2, and 4 μmol/L of RV, respectively. The results revealed that the first polar body (PB1) extrusion rate of the oocytes significantly increased when the RV concentration reached up to 2 μmol/L (<i>p</i> < 0.05). Considering optimum RV concentration of 2 μmol/L, the potential of RV was evaluated in oocytes aged for 24 and 48 h. We used fluorescence microscopy to detect the relative level of reactive oxygen species (ROS), while GHS contents were measured through the enzymatic method. Our results revealed that aged groups (24 h and 48 h) treated with RV (2 μmol/L) showed higher (<i>p</i> < 0.05) ROS fluorescence intensity than the control group, but lower (<i>p</i> < 0.05) than untreated aged groups. The GSH content in untreated aged groups (24 h and 48 h) was lower (<i>p</i> < 0.05) than RV-treated groups, but both groups showed higher levels than the control. Similarly, the relative expression of the genes involved in antioxidant activity (<i>CAT</i>, <i>GPXGSH-Px</i>, and <i>SOD1)</i> in RV-treated groups was lower (<i>p</i> < 0.05) as compared to the control group but higher than that of untreated aged groups. Moreover, the relative mRNA expression of caspase-3 and Bax in RV-treated groups was higher (<i>p</i> < 0.05) than the control group but lower than untreated groups. Furthermore, the expression of <i>Bcl-2</i> in the RV-treated group was significantly lower than control but higher than untreated aged groups. Taken together, our findings revealed that the RV can increase the expression of antioxidant genes by decreasing the level of ROS, and its potent antiapoptotic effects resisted against the decline in mitochondrial membrane potential in aged oocytes.
format	article
author	Benazir Abbasi Yan Dong Rong Rui
author_facet	Benazir Abbasi Yan Dong Rong Rui
author_sort	Benazir Abbasi
title	Resveratrol Hinders Postovulatory Aging by Modulating Oxidative Stress in Porcine Oocytes
title_short	Resveratrol Hinders Postovulatory Aging by Modulating Oxidative Stress in Porcine Oocytes
title_full	Resveratrol Hinders Postovulatory Aging by Modulating Oxidative Stress in Porcine Oocytes
title_fullStr	Resveratrol Hinders Postovulatory Aging by Modulating Oxidative Stress in Porcine Oocytes
title_full_unstemmed	Resveratrol Hinders Postovulatory Aging by Modulating Oxidative Stress in Porcine Oocytes
title_sort	resveratrol hinders postovulatory aging by modulating oxidative stress in porcine oocytes
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/deac7d5ff94446879e8d31f6b686b6c9
work_keys_str_mv	AT benazirabbasi resveratrolhinderspostovulatoryagingbymodulatingoxidativestressinporcineoocytes AT yandong resveratrolhinderspostovulatoryagingbymodulatingoxidativestressinporcineoocytes AT rongrui resveratrolhinderspostovulatoryagingbymodulatingoxidativestressinporcineoocytes
_version_	1718431855248670720

Resveratrol Hinders Postovulatory Aging by Modulating Oxidative Stress in Porcine Oocytes

Ejemplares similares