Peculiarities of the phenotype of T-lymphocytes in the dynamics of the postoperating period in patients with peritonite depending on the outcome of disease

The aim of the study was to investigate the phenotype of blood T-lymphocytes in the dynamics of the postoperative period in patients with widespread purulent peritonitis (WPP) depending on the outcome of the disease. A total of 36 patients aged 30–65 with acute surgical diseases and abdominal injuri...

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Autores principales:	A. A. Savchenko, A. G. Borisov, D. V. Cherdancev, O. V. Pervova, I. V. Kudryavtsev, V. D. Belenjuk
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2019
Materias:	peritonitis outcome of the disease t-lymphocytes t-regulatory cells cytotoxic t-lymphocytes preoperative period postoperative treatment Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/deacd31129b8490383c2b6af67409517
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spelling	oai:doaj.org-article:deacd31129b8490383c2b6af674095172021-11-22T07:09:52ZPeculiarities of the phenotype of T-lymphocytes in the dynamics of the postoperating period in patients with peritonite depending on the outcome of disease2220-76192313-739810.15789/2220-7619-2019-1-115-127https://doaj.org/article/deacd31129b8490383c2b6af674095172019-05-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/627https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398The aim of the study was to investigate the phenotype of blood T-lymphocytes in the dynamics of the postoperative period in patients with widespread purulent peritonitis (WPP) depending on the outcome of the disease. A total of 36 patients aged 30–65 with acute surgical diseases and abdominal injuries complicated by WPP years were examined. Blood sampling was performed before the operation (preoperative period), and also on the 7th, 14th and 21st day of the postoperative period. 40 relatively healthy people of the same age range were examined as a control. Study of the phenotype of blood T-lymphocytes was carried out by the method of 5-color f low cytometry using direct immunof luorescence of whole peripheral blood. Mean levels of fluorescence were used to evaluate the expression levels of surface receptors. It was established that in the preoperative period in patients with WPP regardless of the outcome of the disease against the background of a decrease in the absolute amount of total lymphocytes in the blood the content of T cells increases. The T-lymphocytes of patients with WPP are significantly more intense expressing the CD28 and CD62L receptors than the cells of healthy people. In the preoperative period and within two weeks of postoperative treatment with a favorable outcome of the WPP an increased amount of CD62L+T-lymphocytes is detected in comparison with the indices of patients with an unfavorable outcome of the disease. Other features of the phenotypic composition of T-lymphocytes in patients with a favorable outcome of WPP is an increase in T-regulatory activity which manifests itself both in the preoperative period and within two weeks of postoperative treatment. With a favorable outcome of WPP an increase in the number of activated cytotoxic T-lymphocytes is observed on the 14th day of treatment which with an unfavorable outcome is observed only on the 21st day of treatment. It is assumed that T-cell suppression and activation of cytotoxic T cells are the factors determining a decrease in the intensity of inflammatory processes in WPP and thereby increasing the level of the favorable outcome of the disease. In case of an unfavorable outcome of the disease, postoperative therapy has a weaker or delayed effect on the dynamics of the studied parameters than with a favorable outcome. With a favorable outcome of WPP an increase in the CD3+CD57+cell count is observed already from the second week of treatment and is more pronounced whereas in the case of an adverse outcome of WPP an increase in the level of these cells is observed only at the third week of treatment. However, the level of expression of CD57 on T-lymphocytes is more pronounced throughout the course of the examination with an unfavorable outcome of WPP.A. A. SavchenkoA. G. BorisovD. V. CherdancevO. V. PervovaI. V. KudryavtsevV. D. BelenjukSankt-Peterburg : NIIÈM imeni Pasteraarticleperitonitisoutcome of the diseaset-lymphocytest-regulatory cellscytotoxic t-lymphocytespreoperative periodpostoperative treatmentInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 9, Iss 1, Pp 115-127 (2019)
institution	DOAJ
collection	DOAJ
language	RU
topic	peritonitis outcome of the disease t-lymphocytes t-regulatory cells cytotoxic t-lymphocytes preoperative period postoperative treatment Infectious and parasitic diseases RC109-216
spellingShingle	peritonitis outcome of the disease t-lymphocytes t-regulatory cells cytotoxic t-lymphocytes preoperative period postoperative treatment Infectious and parasitic diseases RC109-216 A. A. Savchenko A. G. Borisov D. V. Cherdancev O. V. Pervova I. V. Kudryavtsev V. D. Belenjuk Peculiarities of the phenotype of T-lymphocytes in the dynamics of the postoperating period in patients with peritonite depending on the outcome of disease
description	The aim of the study was to investigate the phenotype of blood T-lymphocytes in the dynamics of the postoperative period in patients with widespread purulent peritonitis (WPP) depending on the outcome of the disease. A total of 36 patients aged 30–65 with acute surgical diseases and abdominal injuries complicated by WPP years were examined. Blood sampling was performed before the operation (preoperative period), and also on the 7th, 14th and 21st day of the postoperative period. 40 relatively healthy people of the same age range were examined as a control. Study of the phenotype of blood T-lymphocytes was carried out by the method of 5-color f low cytometry using direct immunof luorescence of whole peripheral blood. Mean levels of fluorescence were used to evaluate the expression levels of surface receptors. It was established that in the preoperative period in patients with WPP regardless of the outcome of the disease against the background of a decrease in the absolute amount of total lymphocytes in the blood the content of T cells increases. The T-lymphocytes of patients with WPP are significantly more intense expressing the CD28 and CD62L receptors than the cells of healthy people. In the preoperative period and within two weeks of postoperative treatment with a favorable outcome of the WPP an increased amount of CD62L+T-lymphocytes is detected in comparison with the indices of patients with an unfavorable outcome of the disease. Other features of the phenotypic composition of T-lymphocytes in patients with a favorable outcome of WPP is an increase in T-regulatory activity which manifests itself both in the preoperative period and within two weeks of postoperative treatment. With a favorable outcome of WPP an increase in the number of activated cytotoxic T-lymphocytes is observed on the 14th day of treatment which with an unfavorable outcome is observed only on the 21st day of treatment. It is assumed that T-cell suppression and activation of cytotoxic T cells are the factors determining a decrease in the intensity of inflammatory processes in WPP and thereby increasing the level of the favorable outcome of the disease. In case of an unfavorable outcome of the disease, postoperative therapy has a weaker or delayed effect on the dynamics of the studied parameters than with a favorable outcome. With a favorable outcome of WPP an increase in the CD3+CD57+cell count is observed already from the second week of treatment and is more pronounced whereas in the case of an adverse outcome of WPP an increase in the level of these cells is observed only at the third week of treatment. However, the level of expression of CD57 on T-lymphocytes is more pronounced throughout the course of the examination with an unfavorable outcome of WPP.
format	article
author	A. A. Savchenko A. G. Borisov D. V. Cherdancev O. V. Pervova I. V. Kudryavtsev V. D. Belenjuk
author_facet	A. A. Savchenko A. G. Borisov D. V. Cherdancev O. V. Pervova I. V. Kudryavtsev V. D. Belenjuk
author_sort	A. A. Savchenko
title	Peculiarities of the phenotype of T-lymphocytes in the dynamics of the postoperating period in patients with peritonite depending on the outcome of disease
title_short	Peculiarities of the phenotype of T-lymphocytes in the dynamics of the postoperating period in patients with peritonite depending on the outcome of disease
title_full	Peculiarities of the phenotype of T-lymphocytes in the dynamics of the postoperating period in patients with peritonite depending on the outcome of disease
title_fullStr	Peculiarities of the phenotype of T-lymphocytes in the dynamics of the postoperating period in patients with peritonite depending on the outcome of disease
title_full_unstemmed	Peculiarities of the phenotype of T-lymphocytes in the dynamics of the postoperating period in patients with peritonite depending on the outcome of disease
title_sort	peculiarities of the phenotype of t-lymphocytes in the dynamics of the postoperating period in patients with peritonite depending on the outcome of disease
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2019
url	https://doaj.org/article/deacd31129b8490383c2b6af67409517
work_keys_str_mv	AT aasavchenko peculiaritiesofthephenotypeoftlymphocytesinthedynamicsofthepostoperatingperiodinpatientswithperitonitedependingontheoutcomeofdisease AT agborisov peculiaritiesofthephenotypeoftlymphocytesinthedynamicsofthepostoperatingperiodinpatientswithperitonitedependingontheoutcomeofdisease AT dvcherdancev peculiaritiesofthephenotypeoftlymphocytesinthedynamicsofthepostoperatingperiodinpatientswithperitonitedependingontheoutcomeofdisease AT ovpervova peculiaritiesofthephenotypeoftlymphocytesinthedynamicsofthepostoperatingperiodinpatientswithperitonitedependingontheoutcomeofdisease AT ivkudryavtsev peculiaritiesofthephenotypeoftlymphocytesinthedynamicsofthepostoperatingperiodinpatientswithperitonitedependingontheoutcomeofdisease AT vdbelenjuk peculiaritiesofthephenotypeoftlymphocytesinthedynamicsofthepostoperatingperiodinpatientswithperitonitedependingontheoutcomeofdisease
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Peculiarities of the phenotype of T-lymphocytes in the dynamics of the postoperating period in patients with peritonite depending on the outcome of disease

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