Comparison of Povidone-Iodine and Gentamicin Soak as Scleral Buckle Infection Prophylaxis

Xihui Lin, Brian Le, Patrick Lee, Gary W Abrams, Mark Juzych, Ashok Kumar Department of Ophthalmology, Kresge Eye Institute, Detroit, MI, 48201, USACorrespondence: Xihui LinDepartment of Ophthalmology, Kresge Eye Institute, 4714 St. Antoine Street, Detroit, MI, 48201, USAEmail xihui.lin@wayne.eduObj...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lin X, Le B, Lee P, Abrams GW, Juzych M, Kumar A
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	gentamicin povidone-iodine sterilization Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/deb57b092f2d448ba0fd20bcc89ba375
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:deb57b092f2d448ba0fd20bcc89ba375
record_format	dspace
spelling	oai:doaj.org-article:deb57b092f2d448ba0fd20bcc89ba3752021-12-02T15:55:04ZComparison of Povidone-Iodine and Gentamicin Soak as Scleral Buckle Infection Prophylaxis1177-5483https://doaj.org/article/deb57b092f2d448ba0fd20bcc89ba3752021-05-01T00:00:00Zhttps://www.dovepress.com/comparison-of-povidone-iodine-and-gentamicin-soak-as-scleral-buckle-in-peer-reviewed-fulltext-article-OPTHhttps://doaj.org/toc/1177-5483Xihui Lin, Brian Le, Patrick Lee, Gary W Abrams, Mark Juzych, Ashok Kumar Department of Ophthalmology, Kresge Eye Institute, Detroit, MI, 48201, USACorrespondence: Xihui LinDepartment of Ophthalmology, Kresge Eye Institute, 4714 St. Antoine Street, Detroit, MI, 48201, USAEmail xihui.lin@wayne.eduObjective: To determine the best method of antimicrobial prophylaxis against implanted material-associated infections in the setting of scleral buckle surgery.Design: Experimental study.Participants: Scleral buckle elements were soaked in either gram-positive or polymicrobial broth, while control buckle elements were soaked in PBS only.Methods: Solid silicone and sponge scleral buckle elements were inoculated with common pathogens of the ocular surface, and then soaked in either 1% or 5% povidone-iodine, 1 mg/mL gentamicin solution, or sterile saline for 1, 5, 10, or 15 minutes. Bacteria were then isolated from the buckle elements and cultured for 24 hours.Results: In all gram-positive bacterial conditions, gentamicin solution decreased the bacterial load from 451,666.67 colony-forming units (CFU)/mL to 171,611.11 CFU/mL (p=0.0004). The fractional bacterial survival after soaking in gentamicin was higher for the silicone sponge than band (0.357 vs 0.079, p=0.038). Both 1% and 5% povidone-iodine were able to completely eradicate all gram-positive bacteria of both buckle elements. Only 5% povidone-iodine was able to completely sterilize all microbes on the buckle after soaking in a polymicrobial solution consisting of gram-positive, gram-negative bacteria, and fungi.Conclusion: Povidone-iodine solution was significantly more effective at bacterial eradication compared to gentamicin solution. For all scleral buckle procedures, we recommend soaking the buckle element in 2– 3% povidone-iodine solution before placement and rinsing the ocular surface with the same solution after placement.Keywords: gentamicin, povidone-iodine, sterilizationLin XLe BLee PAbrams GWJuzych MKumar ADove Medical Pressarticlegentamicinpovidone-iodinesterilizationOphthalmologyRE1-994ENClinical Ophthalmology, Vol Volume 15, Pp 2203-2209 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	gentamicin povidone-iodine sterilization Ophthalmology RE1-994
spellingShingle	gentamicin povidone-iodine sterilization Ophthalmology RE1-994 Lin X Le B Lee P Abrams GW Juzych M Kumar A Comparison of Povidone-Iodine and Gentamicin Soak as Scleral Buckle Infection Prophylaxis
description	Xihui Lin, Brian Le, Patrick Lee, Gary W Abrams, Mark Juzych, Ashok Kumar Department of Ophthalmology, Kresge Eye Institute, Detroit, MI, 48201, USACorrespondence: Xihui LinDepartment of Ophthalmology, Kresge Eye Institute, 4714 St. Antoine Street, Detroit, MI, 48201, USAEmail xihui.lin@wayne.eduObjective: To determine the best method of antimicrobial prophylaxis against implanted material-associated infections in the setting of scleral buckle surgery.Design: Experimental study.Participants: Scleral buckle elements were soaked in either gram-positive or polymicrobial broth, while control buckle elements were soaked in PBS only.Methods: Solid silicone and sponge scleral buckle elements were inoculated with common pathogens of the ocular surface, and then soaked in either 1% or 5% povidone-iodine, 1 mg/mL gentamicin solution, or sterile saline for 1, 5, 10, or 15 minutes. Bacteria were then isolated from the buckle elements and cultured for 24 hours.Results: In all gram-positive bacterial conditions, gentamicin solution decreased the bacterial load from 451,666.67 colony-forming units (CFU)/mL to 171,611.11 CFU/mL (p=0.0004). The fractional bacterial survival after soaking in gentamicin was higher for the silicone sponge than band (0.357 vs 0.079, p=0.038). Both 1% and 5% povidone-iodine were able to completely eradicate all gram-positive bacteria of both buckle elements. Only 5% povidone-iodine was able to completely sterilize all microbes on the buckle after soaking in a polymicrobial solution consisting of gram-positive, gram-negative bacteria, and fungi.Conclusion: Povidone-iodine solution was significantly more effective at bacterial eradication compared to gentamicin solution. For all scleral buckle procedures, we recommend soaking the buckle element in 2– 3% povidone-iodine solution before placement and rinsing the ocular surface with the same solution after placement.Keywords: gentamicin, povidone-iodine, sterilization
format	article
author	Lin X Le B Lee P Abrams GW Juzych M Kumar A
author_facet	Lin X Le B Lee P Abrams GW Juzych M Kumar A
author_sort	Lin X
title	Comparison of Povidone-Iodine and Gentamicin Soak as Scleral Buckle Infection Prophylaxis
title_short	Comparison of Povidone-Iodine and Gentamicin Soak as Scleral Buckle Infection Prophylaxis
title_full	Comparison of Povidone-Iodine and Gentamicin Soak as Scleral Buckle Infection Prophylaxis
title_fullStr	Comparison of Povidone-Iodine and Gentamicin Soak as Scleral Buckle Infection Prophylaxis
title_full_unstemmed	Comparison of Povidone-Iodine and Gentamicin Soak as Scleral Buckle Infection Prophylaxis
title_sort	comparison of povidone-iodine and gentamicin soak as scleral buckle infection prophylaxis
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/deb57b092f2d448ba0fd20bcc89ba375
work_keys_str_mv	AT linx comparisonofpovidoneiodineandgentamicinsoakasscleralbuckleinfectionprophylaxis AT leb comparisonofpovidoneiodineandgentamicinsoakasscleralbuckleinfectionprophylaxis AT leep comparisonofpovidoneiodineandgentamicinsoakasscleralbuckleinfectionprophylaxis AT abramsgw comparisonofpovidoneiodineandgentamicinsoakasscleralbuckleinfectionprophylaxis AT juzychm comparisonofpovidoneiodineandgentamicinsoakasscleralbuckleinfectionprophylaxis AT kumara comparisonofpovidoneiodineandgentamicinsoakasscleralbuckleinfectionprophylaxis
_version_	1718385481134112768

Comparison of Povidone-Iodine and Gentamicin Soak as Scleral Buckle Infection Prophylaxis

Ejemplares similares