In Vitro Evaluation of Acellular Collagen Matrices Derived from Porcine Pericardium: Influence of the Sterilization Method on Its Biological Properties

The aim of this study were characterize acellular collagen matrices derived from porcine pericardium (PP) and to evaluate their properties after sterilization by ethylene oxide and gamma ray. PP matrices were subjected to alkaline hydrolysis (AH), and samples were characterized for biological stabil...

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Autores principales:	Rone Aparecido De Grandis, Larissa Natiele Miotto, Luis Eduardo Genaro, Larissa Migliatti Polli, Ana Maria de Guzzi Plepis, Fabiana Tessari Rodrigues, Virginia da Conceição Amaro Martins, Leonardo Pereira Franchi, Raquel Mantuaneli Scarel-Caminaga, Ticiana Sidorenko de Oliveira Capote
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	porcine pericardium tissue regeneration collagen matrices Technology T Electrical engineering. Electronics. Nuclear engineering TK1-9971 Engineering (General). Civil engineering (General) TA1-2040 Microscopy QH201-278.5 Descriptive and experimental mechanics QC120-168.85
Acceso en línea:	https://doaj.org/article/debad3878d864678949b607b5a93c0a1
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spelling	oai:doaj.org-article:debad3878d864678949b607b5a93c0a12021-11-11T17:50:27ZIn Vitro Evaluation of Acellular Collagen Matrices Derived from Porcine Pericardium: Influence of the Sterilization Method on Its Biological Properties10.3390/ma142162551996-1944https://doaj.org/article/debad3878d864678949b607b5a93c0a12021-10-01T00:00:00Zhttps://www.mdpi.com/1996-1944/14/21/6255https://doaj.org/toc/1996-1944The aim of this study were characterize acellular collagen matrices derived from porcine pericardium (PP) and to evaluate their properties after sterilization by ethylene oxide and gamma ray. PP matrices were subjected to alkaline hydrolysis (AH), and samples were characterized for biological stability, membrane thickness measurements, differential scanning calorimetry (DSC) and scanning electron microscopy (SEM). Subsequently, the matrices were frozen, lyophilized and sterilized by ethylene oxide or gamma radiation. For in vitro assays, CHO-K1 cell culture was used and evaluated for cytotoxicity, clonogenic survival assay, genotoxicity and mutagenicity. Analysis of variance (ANOVA) was used, followed by Dunnett’s post-test, with a significance level of 5%. After AH, there was no significant change in matrix thickness. The relative biodegradability of the material after implantation was observed. Morphology and dimensions had small changes after AH. As for cell viability, none of the tested matrices showed a statistically significant difference (<i>p</i> > 0.05; Dunnett) regardless of the sterilization method. Furthermore, it was found that PP matrices did not interfere with the proliferation capacity of CHO-K1 cells (<i>p</i> > 0.05; Dunnett). As for genotoxicity, when sterilized with ethylene oxide (NP, P12 and P24), it showed genotoxic potential, but it was not genotoxic when sterilized by gamma radiation. No mutagenic effects were observed in either group. PP-derived collagen matrices hydrolyzed at different times were not cytotoxic. It is concluded that the best method of sterilization is through gamma radiation, since no significant changes were observed in the properties of the PP matrices.Rone Aparecido De GrandisLarissa Natiele MiottoLuis Eduardo GenaroLarissa Migliatti PolliAna Maria de Guzzi PlepisFabiana Tessari RodriguesVirginia da Conceição Amaro MartinsLeonardo Pereira FranchiRaquel Mantuaneli Scarel-CaminagaTiciana Sidorenko de Oliveira CapoteMDPI AGarticleporcine pericardiumtissue regenerationcollagen matricesTechnologyTElectrical engineering. Electronics. Nuclear engineeringTK1-9971Engineering (General). Civil engineering (General)TA1-2040MicroscopyQH201-278.5Descriptive and experimental mechanicsQC120-168.85ENMaterials, Vol 14, Iss 6255, p 6255 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	porcine pericardium tissue regeneration collagen matrices Technology T Electrical engineering. Electronics. Nuclear engineering TK1-9971 Engineering (General). Civil engineering (General) TA1-2040 Microscopy QH201-278.5 Descriptive and experimental mechanics QC120-168.85
spellingShingle	porcine pericardium tissue regeneration collagen matrices Technology T Electrical engineering. Electronics. Nuclear engineering TK1-9971 Engineering (General). Civil engineering (General) TA1-2040 Microscopy QH201-278.5 Descriptive and experimental mechanics QC120-168.85 Rone Aparecido De Grandis Larissa Natiele Miotto Luis Eduardo Genaro Larissa Migliatti Polli Ana Maria de Guzzi Plepis Fabiana Tessari Rodrigues Virginia da Conceição Amaro Martins Leonardo Pereira Franchi Raquel Mantuaneli Scarel-Caminaga Ticiana Sidorenko de Oliveira Capote In Vitro Evaluation of Acellular Collagen Matrices Derived from Porcine Pericardium: Influence of the Sterilization Method on Its Biological Properties
description	The aim of this study were characterize acellular collagen matrices derived from porcine pericardium (PP) and to evaluate their properties after sterilization by ethylene oxide and gamma ray. PP matrices were subjected to alkaline hydrolysis (AH), and samples were characterized for biological stability, membrane thickness measurements, differential scanning calorimetry (DSC) and scanning electron microscopy (SEM). Subsequently, the matrices were frozen, lyophilized and sterilized by ethylene oxide or gamma radiation. For in vitro assays, CHO-K1 cell culture was used and evaluated for cytotoxicity, clonogenic survival assay, genotoxicity and mutagenicity. Analysis of variance (ANOVA) was used, followed by Dunnett’s post-test, with a significance level of 5%. After AH, there was no significant change in matrix thickness. The relative biodegradability of the material after implantation was observed. Morphology and dimensions had small changes after AH. As for cell viability, none of the tested matrices showed a statistically significant difference (<i>p</i> > 0.05; Dunnett) regardless of the sterilization method. Furthermore, it was found that PP matrices did not interfere with the proliferation capacity of CHO-K1 cells (<i>p</i> > 0.05; Dunnett). As for genotoxicity, when sterilized with ethylene oxide (NP, P12 and P24), it showed genotoxic potential, but it was not genotoxic when sterilized by gamma radiation. No mutagenic effects were observed in either group. PP-derived collagen matrices hydrolyzed at different times were not cytotoxic. It is concluded that the best method of sterilization is through gamma radiation, since no significant changes were observed in the properties of the PP matrices.
format	article
author	Rone Aparecido De Grandis Larissa Natiele Miotto Luis Eduardo Genaro Larissa Migliatti Polli Ana Maria de Guzzi Plepis Fabiana Tessari Rodrigues Virginia da Conceição Amaro Martins Leonardo Pereira Franchi Raquel Mantuaneli Scarel-Caminaga Ticiana Sidorenko de Oliveira Capote
author_facet	Rone Aparecido De Grandis Larissa Natiele Miotto Luis Eduardo Genaro Larissa Migliatti Polli Ana Maria de Guzzi Plepis Fabiana Tessari Rodrigues Virginia da Conceição Amaro Martins Leonardo Pereira Franchi Raquel Mantuaneli Scarel-Caminaga Ticiana Sidorenko de Oliveira Capote
author_sort	Rone Aparecido De Grandis
title	In Vitro Evaluation of Acellular Collagen Matrices Derived from Porcine Pericardium: Influence of the Sterilization Method on Its Biological Properties
title_short	In Vitro Evaluation of Acellular Collagen Matrices Derived from Porcine Pericardium: Influence of the Sterilization Method on Its Biological Properties
title_full	In Vitro Evaluation of Acellular Collagen Matrices Derived from Porcine Pericardium: Influence of the Sterilization Method on Its Biological Properties
title_fullStr	In Vitro Evaluation of Acellular Collagen Matrices Derived from Porcine Pericardium: Influence of the Sterilization Method on Its Biological Properties
title_full_unstemmed	In Vitro Evaluation of Acellular Collagen Matrices Derived from Porcine Pericardium: Influence of the Sterilization Method on Its Biological Properties
title_sort	in vitro evaluation of acellular collagen matrices derived from porcine pericardium: influence of the sterilization method on its biological properties
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/debad3878d864678949b607b5a93c0a1
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In Vitro Evaluation of Acellular Collagen Matrices Derived from Porcine Pericardium: Influence of the Sterilization Method on Its Biological Properties

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