Lipid yield from the diatom Porosira glacialis is determined by solvent choice and number of extractions, independent of cell disruption
Abstract Cell wall disruption is necessary to maximize lipid extraction yields in conventional species of mass-cultivated microalgae. This study investigated the effect of sonication, solvent choice and number of extractions on the lipid yield, lipid class composition and fatty acid composition of t...
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Nature Portfolio
2020
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oai:doaj.org-article:debf25e1e24442c5b18af67a82f665ee2021-12-02T12:42:28ZLipid yield from the diatom Porosira glacialis is determined by solvent choice and number of extractions, independent of cell disruption10.1038/s41598-020-79269-z2045-2322https://doaj.org/article/debf25e1e24442c5b18af67a82f665ee2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79269-zhttps://doaj.org/toc/2045-2322Abstract Cell wall disruption is necessary to maximize lipid extraction yields in conventional species of mass-cultivated microalgae. This study investigated the effect of sonication, solvent choice and number of extractions on the lipid yield, lipid class composition and fatty acid composition of the diatom Porosira glacialis. For comparison, the diatom Odontella aurita and green alga Chlorella vulgaris were included in the study. Sonication effectively disrupted P. glacialis cells, but did not increase the total lipid yield compared to physical stirring (mixing). In all three microalgae, the content of membrane-associated glyco- and phosopholipids in the extracted lipids was strongly dependent on the solvent polarity. A second extraction resulted in higher yields from the microalgae only when polar solvents were used. In conclusion, choice of solvent and number of extractions were the main factors that determined lipid yield and lipid class composition in P. glacialis.Jon Brage SvenningLars DalheimTerje VasskogLucie MatriconBirthe VangRagnar Ludvig OlsenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-10 (2020) |
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Medicine R Science Q Jon Brage Svenning Lars Dalheim Terje Vasskog Lucie Matricon Birthe Vang Ragnar Ludvig Olsen Lipid yield from the diatom Porosira glacialis is determined by solvent choice and number of extractions, independent of cell disruption |
| description |
Abstract Cell wall disruption is necessary to maximize lipid extraction yields in conventional species of mass-cultivated microalgae. This study investigated the effect of sonication, solvent choice and number of extractions on the lipid yield, lipid class composition and fatty acid composition of the diatom Porosira glacialis. For comparison, the diatom Odontella aurita and green alga Chlorella vulgaris were included in the study. Sonication effectively disrupted P. glacialis cells, but did not increase the total lipid yield compared to physical stirring (mixing). In all three microalgae, the content of membrane-associated glyco- and phosopholipids in the extracted lipids was strongly dependent on the solvent polarity. A second extraction resulted in higher yields from the microalgae only when polar solvents were used. In conclusion, choice of solvent and number of extractions were the main factors that determined lipid yield and lipid class composition in P. glacialis. |
| format |
article |
| author |
Jon Brage Svenning Lars Dalheim Terje Vasskog Lucie Matricon Birthe Vang Ragnar Ludvig Olsen |
| author_facet |
Jon Brage Svenning Lars Dalheim Terje Vasskog Lucie Matricon Birthe Vang Ragnar Ludvig Olsen |
| author_sort |
Jon Brage Svenning |
| title |
Lipid yield from the diatom Porosira glacialis is determined by solvent choice and number of extractions, independent of cell disruption |
| title_short |
Lipid yield from the diatom Porosira glacialis is determined by solvent choice and number of extractions, independent of cell disruption |
| title_full |
Lipid yield from the diatom Porosira glacialis is determined by solvent choice and number of extractions, independent of cell disruption |
| title_fullStr |
Lipid yield from the diatom Porosira glacialis is determined by solvent choice and number of extractions, independent of cell disruption |
| title_full_unstemmed |
Lipid yield from the diatom Porosira glacialis is determined by solvent choice and number of extractions, independent of cell disruption |
| title_sort |
lipid yield from the diatom porosira glacialis is determined by solvent choice and number of extractions, independent of cell disruption |
| publisher |
Nature Portfolio |
| publishDate |
2020 |
| url |
https://doaj.org/article/debf25e1e24442c5b18af67a82f665ee |
| work_keys_str_mv |
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