Evaluation of health benefits of sea lamprey (Petromyzon marinus) isolates using in vitro antiinflammatory and antioxidant assays.

Sea lamprey (Petromyzon marinus), a parasitic fish which survives on blood of other fishes, is consumed as a delicacy in many countries. Our earlier studies on sea lamprey compounds that showed potential to deter adult sea lampreys yielded several sterols, glycerides, free fatty acids, amino acids,...

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Autores principales:	Amila A Dissanayake, C Michael Wagner, Muraleedharan G Nair
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dec8c05165184bd59160af8fe90c8900
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spelling	oai:doaj.org-article:dec8c05165184bd59160af8fe90c89002021-12-02T20:04:27ZEvaluation of health benefits of sea lamprey (Petromyzon marinus) isolates using in vitro antiinflammatory and antioxidant assays.1932-620310.1371/journal.pone.0259587https://doaj.org/article/dec8c05165184bd59160af8fe90c89002021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0259587https://doaj.org/toc/1932-6203Sea lamprey (Petromyzon marinus), a parasitic fish which survives on blood of other fishes, is consumed as a delicacy in many countries. Our earlier studies on sea lamprey compounds that showed potential to deter adult sea lampreys yielded several sterols, glycerides, free fatty acids, amino acids, organic acids and nitrogenous compounds. Therefore, this study was to assess the health-benefits of these compounds including additional isolates from HPLC fractions that kept aside due to lack of activity in sea lamprey deterrent assays. In vitro cyclooxygenase enzymes (COX-1 and -2) and lipid peroxidation (LPO) inhibitory assays, respectively, were used to determine antiinflammatory and antioxidant activities. Among the tested sterols, cholesteryl eicosapentaenoate and cholesteryl arachidonate exhibited IC50 values of 14.6 and 17.7 μg/mL for COX-1 and 17.3 and 20.8 μg/mL for COX-2, respectively. Cholesteryl palmitate and cholesteryl oleate showed moderate COX-1 and COX-2 enzyme inhibition at 25 μg/mL. Amino acids arginine, tyrosine, glutamic acid, tryptophan and asparagine also showed moderate COX-1 and COX-2 inhibition at the same concentration. Among the twelve new isolates from fractions that we did not investigate earlier, a novel uracil derivative petromyzonacil showed COX-1 and COX-2 inhibition at 25 μg/mL by 35 and 15%, respectively. Cholesterol esters tested at 25 μg/mL exhibited LPO inhibition between 38 and 82 percent. Amino acids cysteine, methionine, aspartic acid, threonine, tryptophan, histidine, glutamic acid, phenylalanine and tyrosine at 25 μg/mL showed LPO inhibition between 37 and 58% and petromyzonacil by 32%. These assay results indicate that consumption of sea lamprey offer health-benefits in addition to nutritional benefits.Amila A DissanayakeC Michael WagnerMuraleedharan G NairPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11, p e0259587 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Amila A Dissanayake C Michael Wagner Muraleedharan G Nair Evaluation of health benefits of sea lamprey (Petromyzon marinus) isolates using in vitro antiinflammatory and antioxidant assays.
description	Sea lamprey (Petromyzon marinus), a parasitic fish which survives on blood of other fishes, is consumed as a delicacy in many countries. Our earlier studies on sea lamprey compounds that showed potential to deter adult sea lampreys yielded several sterols, glycerides, free fatty acids, amino acids, organic acids and nitrogenous compounds. Therefore, this study was to assess the health-benefits of these compounds including additional isolates from HPLC fractions that kept aside due to lack of activity in sea lamprey deterrent assays. In vitro cyclooxygenase enzymes (COX-1 and -2) and lipid peroxidation (LPO) inhibitory assays, respectively, were used to determine antiinflammatory and antioxidant activities. Among the tested sterols, cholesteryl eicosapentaenoate and cholesteryl arachidonate exhibited IC50 values of 14.6 and 17.7 μg/mL for COX-1 and 17.3 and 20.8 μg/mL for COX-2, respectively. Cholesteryl palmitate and cholesteryl oleate showed moderate COX-1 and COX-2 enzyme inhibition at 25 μg/mL. Amino acids arginine, tyrosine, glutamic acid, tryptophan and asparagine also showed moderate COX-1 and COX-2 inhibition at the same concentration. Among the twelve new isolates from fractions that we did not investigate earlier, a novel uracil derivative petromyzonacil showed COX-1 and COX-2 inhibition at 25 μg/mL by 35 and 15%, respectively. Cholesterol esters tested at 25 μg/mL exhibited LPO inhibition between 38 and 82 percent. Amino acids cysteine, methionine, aspartic acid, threonine, tryptophan, histidine, glutamic acid, phenylalanine and tyrosine at 25 μg/mL showed LPO inhibition between 37 and 58% and petromyzonacil by 32%. These assay results indicate that consumption of sea lamprey offer health-benefits in addition to nutritional benefits.
format	article
author	Amila A Dissanayake C Michael Wagner Muraleedharan G Nair
author_facet	Amila A Dissanayake C Michael Wagner Muraleedharan G Nair
author_sort	Amila A Dissanayake
title	Evaluation of health benefits of sea lamprey (Petromyzon marinus) isolates using in vitro antiinflammatory and antioxidant assays.
title_short	Evaluation of health benefits of sea lamprey (Petromyzon marinus) isolates using in vitro antiinflammatory and antioxidant assays.
title_full	Evaluation of health benefits of sea lamprey (Petromyzon marinus) isolates using in vitro antiinflammatory and antioxidant assays.
title_fullStr	Evaluation of health benefits of sea lamprey (Petromyzon marinus) isolates using in vitro antiinflammatory and antioxidant assays.
title_full_unstemmed	Evaluation of health benefits of sea lamprey (Petromyzon marinus) isolates using in vitro antiinflammatory and antioxidant assays.
title_sort	evaluation of health benefits of sea lamprey (petromyzon marinus) isolates using in vitro antiinflammatory and antioxidant assays.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/dec8c05165184bd59160af8fe90c8900
work_keys_str_mv	AT amilaadissanayake evaluationofhealthbenefitsofsealampreypetromyzonmarinusisolatesusinginvitroantiinflammatoryandantioxidantassays AT cmichaelwagner evaluationofhealthbenefitsofsealampreypetromyzonmarinusisolatesusinginvitroantiinflammatoryandantioxidantassays AT muraleedharangnair evaluationofhealthbenefitsofsealampreypetromyzonmarinusisolatesusinginvitroantiinflammatoryandantioxidantassays
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Evaluation of health benefits of sea lamprey (Petromyzon marinus) isolates using in vitro antiinflammatory and antioxidant assays.

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