The timing of IFNβ production affects early innate responses to Listeria monocytogenes and determines the overall outcome of lethal infection.

Dendritic cells (DCs) and natural killer (NK) cells are essential components of the innate immunity and play a crucial role in the first phase of host defense against infections and tumors. Listeria monocytogenes (Lm) is an intracellular pathogen that colonizes the cytosol of eukaryotic cells. Recen...

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Autores principales:	Francesca Pontiroli, Olivier Dussurget, Ivan Zanoni, Matteo Urbano, Ottavio Beretta, Francesca Granucci, Paola Ricciardi-Castagnoli, Pascale Cossart, Maria Foti
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ded0381a402d4de991896ede3fa97fde
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spelling	oai:doaj.org-article:ded0381a402d4de991896ede3fa97fde2021-11-18T07:08:21ZThe timing of IFNβ production affects early innate responses to Listeria monocytogenes and determines the overall outcome of lethal infection.1932-620310.1371/journal.pone.0043455https://doaj.org/article/ded0381a402d4de991896ede3fa97fde2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22912878/?tool=EBIhttps://doaj.org/toc/1932-6203Dendritic cells (DCs) and natural killer (NK) cells are essential components of the innate immunity and play a crucial role in the first phase of host defense against infections and tumors. Listeria monocytogenes (Lm) is an intracellular pathogen that colonizes the cytosol of eukaryotic cells. Recent findings have shown Lm specifically in splenic CD8a(+) DCs shortly after intravenous infection. We examined gene expression profiles of mouse DCs exposed to Lm to elucidate the molecular mechanisms underlying DCs interaction with Lm. Using a functional genomics approach, we found that Lm infection induced a cluster of late response genes including type I IFNs and interferon responsive genes (IRGs) in DCs. Type I INFs were produced at the maximal level only at 24 h post infection indicating that the regulation of IFNs in the context of Lm infection is delayed compared to the rapid response observed with viral pathogens. We showed that during Lm infection, IFNγ production and cytotoxic activity were severely impaired in NK cells compared to E. coli infection. These defects were restored by providing an exogenous source of IFNβ during the initial phase of bacterial challenge. Moreover, when treated with IFNβ during early infection, NK cells were able to reduce bacterial titer in the spleen and significantly improve survival of infected mice. These findings show that the timing of IFNβ production is fundamental to the efficient control of the bacterium during the early innate phase of Lm infection.Francesca PontiroliOlivier DussurgetIvan ZanoniMatteo UrbanoOttavio BerettaFrancesca GranucciPaola Ricciardi-CastagnoliPascale CossartMaria FotiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e43455 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Francesca Pontiroli Olivier Dussurget Ivan Zanoni Matteo Urbano Ottavio Beretta Francesca Granucci Paola Ricciardi-Castagnoli Pascale Cossart Maria Foti The timing of IFNβ production affects early innate responses to Listeria monocytogenes and determines the overall outcome of lethal infection.
description	Dendritic cells (DCs) and natural killer (NK) cells are essential components of the innate immunity and play a crucial role in the first phase of host defense against infections and tumors. Listeria monocytogenes (Lm) is an intracellular pathogen that colonizes the cytosol of eukaryotic cells. Recent findings have shown Lm specifically in splenic CD8a(+) DCs shortly after intravenous infection. We examined gene expression profiles of mouse DCs exposed to Lm to elucidate the molecular mechanisms underlying DCs interaction with Lm. Using a functional genomics approach, we found that Lm infection induced a cluster of late response genes including type I IFNs and interferon responsive genes (IRGs) in DCs. Type I INFs were produced at the maximal level only at 24 h post infection indicating that the regulation of IFNs in the context of Lm infection is delayed compared to the rapid response observed with viral pathogens. We showed that during Lm infection, IFNγ production and cytotoxic activity were severely impaired in NK cells compared to E. coli infection. These defects were restored by providing an exogenous source of IFNβ during the initial phase of bacterial challenge. Moreover, when treated with IFNβ during early infection, NK cells were able to reduce bacterial titer in the spleen and significantly improve survival of infected mice. These findings show that the timing of IFNβ production is fundamental to the efficient control of the bacterium during the early innate phase of Lm infection.
format	article
author	Francesca Pontiroli Olivier Dussurget Ivan Zanoni Matteo Urbano Ottavio Beretta Francesca Granucci Paola Ricciardi-Castagnoli Pascale Cossart Maria Foti
author_facet	Francesca Pontiroli Olivier Dussurget Ivan Zanoni Matteo Urbano Ottavio Beretta Francesca Granucci Paola Ricciardi-Castagnoli Pascale Cossart Maria Foti
author_sort	Francesca Pontiroli
title	The timing of IFNβ production affects early innate responses to Listeria monocytogenes and determines the overall outcome of lethal infection.
title_short	The timing of IFNβ production affects early innate responses to Listeria monocytogenes and determines the overall outcome of lethal infection.
title_full	The timing of IFNβ production affects early innate responses to Listeria monocytogenes and determines the overall outcome of lethal infection.
title_fullStr	The timing of IFNβ production affects early innate responses to Listeria monocytogenes and determines the overall outcome of lethal infection.
title_full_unstemmed	The timing of IFNβ production affects early innate responses to Listeria monocytogenes and determines the overall outcome of lethal infection.
title_sort	timing of ifnβ production affects early innate responses to listeria monocytogenes and determines the overall outcome of lethal infection.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/ded0381a402d4de991896ede3fa97fde
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The timing of IFNβ production affects early innate responses to Listeria monocytogenes and determines the overall outcome of lethal infection.

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