X-ray phase nanotomography resolves the 3D human bone ultrastructure.

Bone strength and failure are increasingly thought to be due to ultrastructural properties, such as the morphology of the lacuno-canalicular network, the collagen fiber orientation and the mineralization on the nanoscale. However, these properties have not been studied in 3D so far. Here we report t...

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Autores principales:	Max Langer, Alexandra Pacureanu, Heikki Suhonen, Quentin Grimal, Peter Cloetens, Françoise Peyrin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ded0c2ab3a894686b05a13f4e80a0967
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id	oai:doaj.org-article:ded0c2ab3a894686b05a13f4e80a0967
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spelling	oai:doaj.org-article:ded0c2ab3a894686b05a13f4e80a09672021-11-18T07:07:17ZX-ray phase nanotomography resolves the 3D human bone ultrastructure.1932-620310.1371/journal.pone.0035691https://doaj.org/article/ded0c2ab3a894686b05a13f4e80a09672012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22952569/?tool=EBIhttps://doaj.org/toc/1932-6203Bone strength and failure are increasingly thought to be due to ultrastructural properties, such as the morphology of the lacuno-canalicular network, the collagen fiber orientation and the mineralization on the nanoscale. However, these properties have not been studied in 3D so far. Here we report the investigation of the human bone ultrastructure with X-ray phase nanotomography, which now provides the required sensitivity, spatial resolution and field of view. The 3D organization of the lacuno-canalicular network is studied in detail over several cells in osteonal and interstitial tissue. Nanoscale density variations are revealed and show that the cement line separating these tissues is hypermineralized. Finally, we show that the collagen fibers are organized as a twisted plywood structure in 3D.Max LangerAlexandra PacureanuHeikki SuhonenQuentin GrimalPeter CloetensFrançoise PeyrinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e35691 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Max Langer Alexandra Pacureanu Heikki Suhonen Quentin Grimal Peter Cloetens Françoise Peyrin X-ray phase nanotomography resolves the 3D human bone ultrastructure.
description	Bone strength and failure are increasingly thought to be due to ultrastructural properties, such as the morphology of the lacuno-canalicular network, the collagen fiber orientation and the mineralization on the nanoscale. However, these properties have not been studied in 3D so far. Here we report the investigation of the human bone ultrastructure with X-ray phase nanotomography, which now provides the required sensitivity, spatial resolution and field of view. The 3D organization of the lacuno-canalicular network is studied in detail over several cells in osteonal and interstitial tissue. Nanoscale density variations are revealed and show that the cement line separating these tissues is hypermineralized. Finally, we show that the collagen fibers are organized as a twisted plywood structure in 3D.
format	article
author	Max Langer Alexandra Pacureanu Heikki Suhonen Quentin Grimal Peter Cloetens Françoise Peyrin
author_facet	Max Langer Alexandra Pacureanu Heikki Suhonen Quentin Grimal Peter Cloetens Françoise Peyrin
author_sort	Max Langer
title	X-ray phase nanotomography resolves the 3D human bone ultrastructure.
title_short	X-ray phase nanotomography resolves the 3D human bone ultrastructure.
title_full	X-ray phase nanotomography resolves the 3D human bone ultrastructure.
title_fullStr	X-ray phase nanotomography resolves the 3D human bone ultrastructure.
title_full_unstemmed	X-ray phase nanotomography resolves the 3D human bone ultrastructure.
title_sort	x-ray phase nanotomography resolves the 3d human bone ultrastructure.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/ded0c2ab3a894686b05a13f4e80a0967
work_keys_str_mv	AT maxlanger xrayphasenanotomographyresolvesthe3dhumanboneultrastructure AT alexandrapacureanu xrayphasenanotomographyresolvesthe3dhumanboneultrastructure AT heikkisuhonen xrayphasenanotomographyresolvesthe3dhumanboneultrastructure AT quentingrimal xrayphasenanotomographyresolvesthe3dhumanboneultrastructure AT petercloetens xrayphasenanotomographyresolvesthe3dhumanboneultrastructure AT francoisepeyrin xrayphasenanotomographyresolvesthe3dhumanboneultrastructure
_version_	1718423948682592256

X-ray phase nanotomography resolves the 3D human bone ultrastructure.

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