Development of Hydrolysis Probe-Based qPCR Assays for <i>Panax ginseng</i> and <i>Panax quinquefolius</i> for Detection of Adulteration in Ginseng Herbal Products
Authentication of <i>Panax ginseng</i> and <i>Panax quinquefolius</i> products is important to be able to mitigate instances of adulteration and substitution that exist within the international supply chain of ginseng. To address this issue, species-specific hydrolysis probe...
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| Autores principales: | , , , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/ded4dc2c73f74c80a2b6047a7307ae47 |
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| Sumario: | Authentication of <i>Panax ginseng</i> and <i>Panax quinquefolius</i> products is important to be able to mitigate instances of adulteration and substitution that exist within the international supply chain of ginseng. To address this issue, species-specific hydrolysis probe qPCR assays were developed and validated for both <i>P. ginseng</i> and <i>P. quinquefolius</i> herbal dietary supplements. Performance of the probe-based assays was evaluated using analytical validation criteria, which included evaluation of: (1) specificity, in selectively identifying the target species; (2) sensitivity, in detecting the lowest amount of the target material; and (3) repeatability and reproducibility of the method in detecting the target species in raw materials on a real-time PCR platform (reliability). The species-specific probes were developed and successfully passed the validation criteria with 100% specificity, 80–120% efficiency and 100% reliability. The methods developed in this study are fit for purpose, rapid, and easy to implement in quality assurance programs; authentication of ginseng herbal supplements is possible, even with extracts where DNA is fragmented and of low quality and quantity. |
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