Development of Hydrolysis Probe-Based qPCR Assays for Panax ginseng and Panax quinquefolius for Detection of Adulteration in Ginseng Herbal Products

Authentication of Panax ginseng and Panax quinquefolius products is important to be able to mitigate instances of adulteration and substitution that exist within the international supply chain of ginseng. To address this issue, species-specific hydrolysis probe...

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Autores principales:	Prasad Kesanakurti, Subramanyam Ragupathy, Adam C. Faller, Dhivya Shanmughanandhan, Francesco Buongiorno, Isabella Della Noce, Zhengfei Lu, Yanjun Zhang, Steven G. Newmaster
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	panax ginseng panax quinquefolius ginseng herbal medicine method validation Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/ded4dc2c73f74c80a2b6047a7307ae47
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spelling	oai:doaj.org-article:ded4dc2c73f74c80a2b6047a7307ae472021-11-25T17:34:22ZDevelopment of Hydrolysis Probe-Based qPCR Assays for <i>Panax ginseng</i> and <i>Panax quinquefolius</i> for Detection of Adulteration in Ginseng Herbal Products10.3390/foods101127052304-8158https://doaj.org/article/ded4dc2c73f74c80a2b6047a7307ae472021-11-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2705https://doaj.org/toc/2304-8158Authentication of <i>Panax ginseng</i> and <i>Panax quinquefolius</i> products is important to be able to mitigate instances of adulteration and substitution that exist within the international supply chain of ginseng. To address this issue, species-specific hydrolysis probe qPCR assays were developed and validated for both <i>P. ginseng</i> and <i>P. quinquefolius</i> herbal dietary supplements. Performance of the probe-based assays was evaluated using analytical validation criteria, which included evaluation of: (1) specificity, in selectively identifying the target species; (2) sensitivity, in detecting the lowest amount of the target material; and (3) repeatability and reproducibility of the method in detecting the target species in raw materials on a real-time PCR platform (reliability). The species-specific probes were developed and successfully passed the validation criteria with 100% specificity, 80–120% efficiency and 100% reliability. The methods developed in this study are fit for purpose, rapid, and easy to implement in quality assurance programs; authentication of ginseng herbal supplements is possible, even with extracts where DNA is fragmented and of low quality and quantity.Prasad KesanakurtiSubramanyam RagupathyAdam C. FallerDhivya ShanmughanandhanFrancesco BuongiornoIsabella Della NoceZhengfei LuYanjun ZhangSteven G. NewmasterMDPI AGarticlepanax ginsengpanax quinquefoliusginsengherbal medicinemethod validationChemical technologyTP1-1185ENFoods, Vol 10, Iss 2705, p 2705 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	panax ginseng panax quinquefolius ginseng herbal medicine method validation Chemical technology TP1-1185
spellingShingle	panax ginseng panax quinquefolius ginseng herbal medicine method validation Chemical technology TP1-1185 Prasad Kesanakurti Subramanyam Ragupathy Adam C. Faller Dhivya Shanmughanandhan Francesco Buongiorno Isabella Della Noce Zhengfei Lu Yanjun Zhang Steven G. Newmaster Development of Hydrolysis Probe-Based qPCR Assays for <i>Panax ginseng</i> and <i>Panax quinquefolius</i> for Detection of Adulteration in Ginseng Herbal Products
description	Authentication of <i>Panax ginseng</i> and <i>Panax quinquefolius</i> products is important to be able to mitigate instances of adulteration and substitution that exist within the international supply chain of ginseng. To address this issue, species-specific hydrolysis probe qPCR assays were developed and validated for both <i>P. ginseng</i> and <i>P. quinquefolius</i> herbal dietary supplements. Performance of the probe-based assays was evaluated using analytical validation criteria, which included evaluation of: (1) specificity, in selectively identifying the target species; (2) sensitivity, in detecting the lowest amount of the target material; and (3) repeatability and reproducibility of the method in detecting the target species in raw materials on a real-time PCR platform (reliability). The species-specific probes were developed and successfully passed the validation criteria with 100% specificity, 80–120% efficiency and 100% reliability. The methods developed in this study are fit for purpose, rapid, and easy to implement in quality assurance programs; authentication of ginseng herbal supplements is possible, even with extracts where DNA is fragmented and of low quality and quantity.
format	article
author	Prasad Kesanakurti Subramanyam Ragupathy Adam C. Faller Dhivya Shanmughanandhan Francesco Buongiorno Isabella Della Noce Zhengfei Lu Yanjun Zhang Steven G. Newmaster
author_facet	Prasad Kesanakurti Subramanyam Ragupathy Adam C. Faller Dhivya Shanmughanandhan Francesco Buongiorno Isabella Della Noce Zhengfei Lu Yanjun Zhang Steven G. Newmaster
author_sort	Prasad Kesanakurti
title	Development of Hydrolysis Probe-Based qPCR Assays for <i>Panax ginseng</i> and <i>Panax quinquefolius</i> for Detection of Adulteration in Ginseng Herbal Products
title_short	Development of Hydrolysis Probe-Based qPCR Assays for <i>Panax ginseng</i> and <i>Panax quinquefolius</i> for Detection of Adulteration in Ginseng Herbal Products
title_full	Development of Hydrolysis Probe-Based qPCR Assays for <i>Panax ginseng</i> and <i>Panax quinquefolius</i> for Detection of Adulteration in Ginseng Herbal Products
title_fullStr	Development of Hydrolysis Probe-Based qPCR Assays for <i>Panax ginseng</i> and <i>Panax quinquefolius</i> for Detection of Adulteration in Ginseng Herbal Products
title_full_unstemmed	Development of Hydrolysis Probe-Based qPCR Assays for <i>Panax ginseng</i> and <i>Panax quinquefolius</i> for Detection of Adulteration in Ginseng Herbal Products
title_sort	development of hydrolysis probe-based qpcr assays for <i>panax ginseng</i> and <i>panax quinquefolius</i> for detection of adulteration in ginseng herbal products
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ded4dc2c73f74c80a2b6047a7307ae47
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Development of Hydrolysis Probe-Based qPCR Assays for <i>Panax ginseng</i> and <i>Panax quinquefolius</i> for Detection of Adulteration in Ginseng Herbal Products

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