Optical density based quantification of total haemoglobin concentrations with spectroscopic optical coherence tomography

Abstract Spectroscopic optical coherence tomography (sOCT) has emerged as a new possibility for non-invasive quantification of total haemoglobin concentrations [tHb]. Recently, we demonstrated that [tHb] measured in ex-vivo human whole-blood with a conventional sOCT system achieves a precision of 9....

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Autores principales:	Carlos Cuartas-Vélez, Colin Veenstra, Saskia Kruitwagen, Wilma Petersen, Nienke Bosschaart
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dedb02e3206a48e68ab6927c2b5c18c5
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spelling	oai:doaj.org-article:dedb02e3206a48e68ab6927c2b5c18c52021-12-02T13:39:55ZOptical density based quantification of total haemoglobin concentrations with spectroscopic optical coherence tomography10.1038/s41598-021-88063-42045-2322https://doaj.org/article/dedb02e3206a48e68ab6927c2b5c18c52021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88063-4https://doaj.org/toc/2045-2322Abstract Spectroscopic optical coherence tomography (sOCT) has emerged as a new possibility for non-invasive quantification of total haemoglobin concentrations [tHb]. Recently, we demonstrated that [tHb] measured in ex-vivo human whole-blood with a conventional sOCT system achieves a precision of 9.10 g/dL with a bias of 1.50 g/dL. This precision improved by acquiring data with a combination of focus tracking and zero-delay acquisition (FZA) that compensated for experimental limitations, increasing to 3.80 g/dL with a bias of 1.50 g/dL. Nevertheless, sOCT precision should improve at least to $$\sim 2$$ ∼ 2 g/dL to be clinically relevant. Therefore, sOCT-based [tHb] determinations require the development of new analysis methods that reduce the variability of [tHb] estimations. In this work, we aim to increase sOCT precision by retrieving the [tHb] content from a numerical optimisation of the optical density (OD), while considering the blood absorption flattening effect. The OD-based approach simplifies previous two-step Lambert–Beer fitting approaches to a single step, thereby reducing errors during the fitting procedure. We validated our model with ex-vivo [tHb] measurements on flowing whole-blood samples in the clinical range (7–23 g/dL). Our results show that, with the new model, conventional sOCT can determine [tHb] with a precision of 3.09 g/dL and a bias of 0.86 g/dL compared to a commercial blood analyser. We present further precision improvement by combining the OD methodology with FZA, leading to a precision of 2.08 g/dL with a bias of 0.46 g/dL.Carlos Cuartas-VélezColin VeenstraSaskia KruitwagenWilma PetersenNienke BosschaartNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Carlos Cuartas-Vélez Colin Veenstra Saskia Kruitwagen Wilma Petersen Nienke Bosschaart Optical density based quantification of total haemoglobin concentrations with spectroscopic optical coherence tomography
description	Abstract Spectroscopic optical coherence tomography (sOCT) has emerged as a new possibility for non-invasive quantification of total haemoglobin concentrations [tHb]. Recently, we demonstrated that [tHb] measured in ex-vivo human whole-blood with a conventional sOCT system achieves a precision of 9.10 g/dL with a bias of 1.50 g/dL. This precision improved by acquiring data with a combination of focus tracking and zero-delay acquisition (FZA) that compensated for experimental limitations, increasing to 3.80 g/dL with a bias of 1.50 g/dL. Nevertheless, sOCT precision should improve at least to $$\sim 2$$ ∼ 2 g/dL to be clinically relevant. Therefore, sOCT-based [tHb] determinations require the development of new analysis methods that reduce the variability of [tHb] estimations. In this work, we aim to increase sOCT precision by retrieving the [tHb] content from a numerical optimisation of the optical density (OD), while considering the blood absorption flattening effect. The OD-based approach simplifies previous two-step Lambert–Beer fitting approaches to a single step, thereby reducing errors during the fitting procedure. We validated our model with ex-vivo [tHb] measurements on flowing whole-blood samples in the clinical range (7–23 g/dL). Our results show that, with the new model, conventional sOCT can determine [tHb] with a precision of 3.09 g/dL and a bias of 0.86 g/dL compared to a commercial blood analyser. We present further precision improvement by combining the OD methodology with FZA, leading to a precision of 2.08 g/dL with a bias of 0.46 g/dL.
format	article
author	Carlos Cuartas-Vélez Colin Veenstra Saskia Kruitwagen Wilma Petersen Nienke Bosschaart
author_facet	Carlos Cuartas-Vélez Colin Veenstra Saskia Kruitwagen Wilma Petersen Nienke Bosschaart
author_sort	Carlos Cuartas-Vélez
title	Optical density based quantification of total haemoglobin concentrations with spectroscopic optical coherence tomography
title_short	Optical density based quantification of total haemoglobin concentrations with spectroscopic optical coherence tomography
title_full	Optical density based quantification of total haemoglobin concentrations with spectroscopic optical coherence tomography
title_fullStr	Optical density based quantification of total haemoglobin concentrations with spectroscopic optical coherence tomography
title_full_unstemmed	Optical density based quantification of total haemoglobin concentrations with spectroscopic optical coherence tomography
title_sort	optical density based quantification of total haemoglobin concentrations with spectroscopic optical coherence tomography
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/dedb02e3206a48e68ab6927c2b5c18c5
work_keys_str_mv	AT carloscuartasvelez opticaldensitybasedquantificationoftotalhaemoglobinconcentrationswithspectroscopicopticalcoherencetomography AT colinveenstra opticaldensitybasedquantificationoftotalhaemoglobinconcentrationswithspectroscopicopticalcoherencetomography AT saskiakruitwagen opticaldensitybasedquantificationoftotalhaemoglobinconcentrationswithspectroscopicopticalcoherencetomography AT wilmapetersen opticaldensitybasedquantificationoftotalhaemoglobinconcentrationswithspectroscopicopticalcoherencetomography AT nienkebosschaart opticaldensitybasedquantificationoftotalhaemoglobinconcentrationswithspectroscopicopticalcoherencetomography
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Optical density based quantification of total haemoglobin concentrations with spectroscopic optical coherence tomography

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