Exploring the taxonomical and functional profile of As Burgas hot spring focusing on thermostable β-galactosidases

Abstract In the present study we investigate the microbial community inhabiting As Burgas geothermal spring, located in Ourense (Galicia, Spain). The approximately 23 Gbp of Illumina sequences generated for each replicate revealed a complex microbial community dominated by Bacteria in which Proteoba...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	María-Eugenia DeCastro, Michael P. Doane, Elizabeth Ann Dinsdale, Esther Rodríguez-Belmonte, María-Isabel González-Siso
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/dedbadce5f1b41e9967159b1694085e0
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:dedbadce5f1b41e9967159b1694085e0
record_format	dspace
spelling	oai:doaj.org-article:dedbadce5f1b41e9967159b1694085e02021-12-02T15:08:22ZExploring the taxonomical and functional profile of As Burgas hot spring focusing on thermostable β-galactosidases10.1038/s41598-020-80489-62045-2322https://doaj.org/article/dedbadce5f1b41e9967159b1694085e02021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80489-6https://doaj.org/toc/2045-2322Abstract In the present study we investigate the microbial community inhabiting As Burgas geothermal spring, located in Ourense (Galicia, Spain). The approximately 23 Gbp of Illumina sequences generated for each replicate revealed a complex microbial community dominated by Bacteria in which Proteobacteria and Aquificae were the two prevalent phyla. An association between the two most prevalent genera, Thermus and Hydrogenobacter, was suggested by the relationship of their metabolism. The high relative abundance of sequences involved in the Calvin–Benson cycle and the reductive TCA cycle unveils the dominance of an autotrophic population. Important pathways from the nitrogen and sulfur cycle are potentially taking place in As Burgas hot spring. In the assembled reads, two complete ORFs matching GH2 beta-galactosidases were found. To assess their functional characterization, the two ORFs were cloned and overexpressed in E. coli. The pTsbg enzyme had activity towards o-Nitrophenyl-β-d-galactopyranoside (ONPG) and p-Nitrophenyl-β-d-fucopyranoside, with high thermal stability and showing maximal activity at 85 °C and pH 6, nevertheless the enzyme failed to hydrolyze lactose. The other enzyme, Tsbg, was unable to hydrolyze even ONPG or lactose. This finding highlights the challenge of finding novel active enzymes based only on their sequence.María-Eugenia DeCastroMichael P. DoaneElizabeth Ann DinsdaleEsther Rodríguez-BelmonteMaría-Isabel González-SisoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q María-Eugenia DeCastro Michael P. Doane Elizabeth Ann Dinsdale Esther Rodríguez-Belmonte María-Isabel González-Siso Exploring the taxonomical and functional profile of As Burgas hot spring focusing on thermostable β-galactosidases
description	Abstract In the present study we investigate the microbial community inhabiting As Burgas geothermal spring, located in Ourense (Galicia, Spain). The approximately 23 Gbp of Illumina sequences generated for each replicate revealed a complex microbial community dominated by Bacteria in which Proteobacteria and Aquificae were the two prevalent phyla. An association between the two most prevalent genera, Thermus and Hydrogenobacter, was suggested by the relationship of their metabolism. The high relative abundance of sequences involved in the Calvin–Benson cycle and the reductive TCA cycle unveils the dominance of an autotrophic population. Important pathways from the nitrogen and sulfur cycle are potentially taking place in As Burgas hot spring. In the assembled reads, two complete ORFs matching GH2 beta-galactosidases were found. To assess their functional characterization, the two ORFs were cloned and overexpressed in E. coli. The pTsbg enzyme had activity towards o-Nitrophenyl-β-d-galactopyranoside (ONPG) and p-Nitrophenyl-β-d-fucopyranoside, with high thermal stability and showing maximal activity at 85 °C and pH 6, nevertheless the enzyme failed to hydrolyze lactose. The other enzyme, Tsbg, was unable to hydrolyze even ONPG or lactose. This finding highlights the challenge of finding novel active enzymes based only on their sequence.
format	article
author	María-Eugenia DeCastro Michael P. Doane Elizabeth Ann Dinsdale Esther Rodríguez-Belmonte María-Isabel González-Siso
author_facet	María-Eugenia DeCastro Michael P. Doane Elizabeth Ann Dinsdale Esther Rodríguez-Belmonte María-Isabel González-Siso
author_sort	María-Eugenia DeCastro
title	Exploring the taxonomical and functional profile of As Burgas hot spring focusing on thermostable β-galactosidases
title_short	Exploring the taxonomical and functional profile of As Burgas hot spring focusing on thermostable β-galactosidases
title_full	Exploring the taxonomical and functional profile of As Burgas hot spring focusing on thermostable β-galactosidases
title_fullStr	Exploring the taxonomical and functional profile of As Burgas hot spring focusing on thermostable β-galactosidases
title_full_unstemmed	Exploring the taxonomical and functional profile of As Burgas hot spring focusing on thermostable β-galactosidases
title_sort	exploring the taxonomical and functional profile of as burgas hot spring focusing on thermostable β-galactosidases
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/dedbadce5f1b41e9967159b1694085e0
work_keys_str_mv	AT mariaeugeniadecastro exploringthetaxonomicalandfunctionalprofileofasburgashotspringfocusingonthermostablebgalactosidases AT michaelpdoane exploringthetaxonomicalandfunctionalprofileofasburgashotspringfocusingonthermostablebgalactosidases AT elizabethanndinsdale exploringthetaxonomicalandfunctionalprofileofasburgashotspringfocusingonthermostablebgalactosidases AT estherrodriguezbelmonte exploringthetaxonomicalandfunctionalprofileofasburgashotspringfocusingonthermostablebgalactosidases AT mariaisabelgonzalezsiso exploringthetaxonomicalandfunctionalprofileofasburgashotspringfocusingonthermostablebgalactosidases
_version_	1718388220515844096

Exploring the taxonomical and functional profile of As Burgas hot spring focusing on thermostable β-galactosidases

Ejemplares similares